Indonesian Propolis Reduces Malondialdehyde Level and Increase Osteoblast Cell Number in Wistar Rats with Orthodontic Tooth Movement
DOI:
https://doi.org/10.3889/oamjms.2020.3984Keywords:
Indonesian propolis, Malondialdehyde, Osteoblast, Orthodontic tooth movementAbstract
AIM: The aim of this study was to determine the antioxidant activity of Indonesian propolis gel 5% in Wistar rats alveolar bone, toward malondialdehyde serum levels and osteoblast cells number caused by orthodontic tooth movement (OTM).
METHODS: This was an experimental study using the post-test only group design. The samples were 28 male Wistar rats, divided into four groups: G1 (control group) – group without OTM and without propolis, G2 – group without OTM and with propolis, G3 – group with OTM and without propolis, and G4 – group with OTM and with propolis. Propolis available in the form of 5% gel and 30 gf helical spring force of OTM applied. Spring was applied in rat maxilla incisors. OTM treatment was given 17 days, and on day 18, blood samples were taken for the measurement of malondialdehyde levels, then tested using the ELISA test. Variable of osteoblast was calculated histologically using hematoxylin-eosin staining. The data of malondialdehyde level and the osteoblast number obtained were tested using one-way ANOVA.
RESULTS: The result indicated that osteoblast number was higher with propolis application compared to those without propolis in the control group and orthodontic tooth treatment group (G2>G1, 23.97 ± 2.95 vs. 18.63 ± 3.04 and G4>G3, 34.17 ± 5.57 vs. 28.26 ± 2.62) with significant difference (p < 0.05). Propolis application also reduces malondialdehyde serum level when compared to both groups without propolis (control and OTM group) (G2<G1, 1.02 ± 0.18 nmol/ml vs. 1.55 ± 0.24 nmol/ml and G4<G3 1.29 ± 0.22 nmol/ml vs. 1.83 ± 0.21 nmol/ml) and significantly different (p < 0.05). OTM increased the malondialdehyde level compared to the control group, with a significant difference (p < 0.05).
CONCLUSION: Propolis gel 5% application can reduce malondialdehyde serum level and could increase the number of osteoblast.
Downloads
Metrics
Plum Analytics Artifact Widget Block
References
Andrade IJ, Silvana RA, Paulo EA. Inflammation and tooth movement: The role of cytokines, chemokines, and growth factors. Semin Orthod. 2012;18(4):257-69.
Khrisnan V, Davidovitch Z. Cellular, molecular, and tissue-level reactions to orthodontic force. Am J Orthod Dentofac Orthop. 2006;129(469e):1-32.
Khrisnan V, Davidovitch Z. Biological Mechanism of Tooth Movement. 3th ed. New York: John Wiley & Son Ltd.; 2015.
Aydin E, Hipokur C, Misir S, Yeler H. Effect of propolis on oxidative stress in rabbits undergoing implant surgery. Cumhuriyet Dent J. 2018;21(2):136-44. https://doi.org/10.7126/cumudj.356554
Toreti VC, Sato HH, Pastore GM, Park YK. Recent progress of propolis for it’s biological and chemical compositions and its botanical origin. Evid Based Complement Alternat Med. 2013;2013:697390. https://doi.org/10.1155/2013/697390 PMid:23737843
Guney A, Karaman I, Mithat OM, Yever MB. Effect of propolis on fracture healing: An experimental study. Phytother Res. 2011;25(11):1648-52. https://doi.org/10.1002/ptr.3470 PMid:21425375
Park YK, Alencar SM, Aguiar CL. Botanical origin and chemical composition of Brazilian propolis. J Agric Food Chem. 2005;50(9):2502-6. https://doi.org/10.1021/jf011432b PMid:11958612
Wiryowidagdo SS, Simanjuntak P, Heffen WL. Chemical composition of propolis from different regions in java and their cytotoxic activity. Am J Biochem Biotech. 2009;25(4):180-3.
Wiwekowati W, Astawa P, Jawi IM, Sabir A. Antioxidant activity of Apis mellifera sp. propolis extract from java (Indonesia). IRJEIS. 2017;3(5):18-23. https://doi.org/10.21744/irjeis.v3i5.530
Maulana M, Hikmah N, Shita AD, Permatasari N, Widyarti S. The effect of different orthodontic force on MMP 9 expression in a rat diabetic model. J Trop Life Sci. 2014;4(2):89-95. https://doi. org/10.11594/jtls.04.02.02
Altan BA, Kara IM, Nalcaci R, Ozan F, Erdogan SM, Ozkut MM, et al. Systemic propolis stimulates new bone formation at the expanded suture. Angle Orthod. 2013;83(2):286-91. https://doi. org/10.2319/032612-253.1 PMid:22906401
Bereket C, Ozan F, Sener I, Tek M, Altunkaynak BZ, Semirgin SU, et al. Propolis accelerates the consolidation phase in distraction osteogenesis. J Craniofac Surg. 2014;25(5):1912-6. https://doi. org/10.1097/scs.0000000000000946 PMid:25203585
Quadras DD, Nayak US, Kumari S, Pujari P. In vivo analysis of lipid peroxidation and total antioxidant status in subjects treated with stainless steel orthodontic appliances. J Health Sci. 2013;3(3):83-6.
Presetyo DH, Suparyanti EL, Guntur HA. Ekstrak etanol propolis isolat menurunkan derajat inflamasi dan kadar malondialdehid pada serum tikus model Sepsis. MKB. 2013;45(3):161-6. https:// doi.org/10.15395/mkb.v45n3.146
Hairrudin, Helianti D. Efek protektif propolis dalam mencegah stres oksidatif akibat aktifitas fisik berat (swimming stres). J Ilmu Dasar. 2019;10(2):207-11. https://doi.org/10.21776/ ub.jkb.2012.027.02.2
Hyunchu J, Kim H, Choi D, Kim D, Park SY, Kim YJ, et al. Quercetin activates an angiogenic pathway, HIF-1-VEGF, by inhibiting HIF-prolyl hydroxylase: A structural analysis of quercetin for inhibiting HIF-prolyl hydroxylase. Mol Pharm. 2007;6:1676-84. https://doi.org/10.1124/mol.107.034041104
Downloads
Published
How to Cite
License
Copyright (c) 2020 Wiwekowati Wiwekowati, M. Taha Ma’ruf, Surwandi Walianto, Ardo Sabir, I Putu Eka Widyadharma (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
http://creativecommons.org/licenses/by-nc/4.0
