The Effect of Glutamine Supplementation to Fecal Calprotectin and Tumor Necrosis Factor-α Levels in the Fecal of Rats with Acute-Induced Diarrhea
DOI:
https://doi.org/10.3889/oamjms.2020.4046Keywords:
Glutamine, Fecal calprotectin, TNF-αAbstract
BACKGROUND: Glutamine is a non-essential amino acid as the main fuel in the gastrointestinal mucosa. By its various gastrointestinal functions, glutamine is thought to increase the protection of the intestinal mucosa against local or systemic injury from diarrhea.
AIM: This study aimed to determine the relationship between glutamine supplementation and fecal calprotectin and tumor necrosis factor-α (TNF-α) in the fecal of rats with acute and chronic diarrhea induced by EPEC.
MATERIALS AND METHODS: The research was conducted in an experimental laboratory with a randomized post-test only control group design. A total of 30 Rattus norvegicus strain Wistar were divided into five groups. The treatment group was induced to have diarrhea using EPEC at a dose of 108 CFU/ml, after each group diagnosed by acute and chronic diarrhea, followed by glutamine supplementation at a dose of 810 mg/200 g for 14 days. The inflammatory cytoknies which is fecal calprotectin and TNF-α, of each group was assessed and the p value was measured by one-way ANOVA and post hoc Bonferroni test to find out the relationship between these variables.
RESULT: Supplementation of glutamine for 14 days can reduce fecal calprotectin levels in chronic diarrhea and reduce TNF-α level in rats with acute and chronic diarrhea.
CONCLUSION: Glutamine supplementation has an effect on the fecal calprotectin and TNF-α of rats with acute and chronic diarrhea.
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Copyright (c) 2020 Deddy Satriya Putra, Fadil Oenzil, Eryati Darwin, Hafni Bachtiar (Author)
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