Association between Luteinizing Hormone/Choriogonadotropin Receptor Ins18LQ Gene Polymorphism and Polycystic Ovary Syndrome
DOI:
https://doi.org/10.3889/oamjms.2020.4182Keywords:
LHCGR, ins18LQ gene polymorphism, PCOS, Association, GeneticAbstract
BACKGROUND: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders of women in the childbearing period. However, its pathophysiology is still unclear. Certain polymorphisms of the luteinizing hormone/choriogonadotropin receptor (LHCGR) genes may lead to changes in the bioactivity of this hormone. The important functional role of LHCGR in the metabolism of androgen and ovulation, the LHCGR gene variant, may be related to the risk of PCOS.
AIM: The aim of this study was to evaluate the association between LHCGR Ins18LQ gene polymorphism and PCOS.
METHODS: A case–control study was performed in women with PCOS and non-PCOS from May 2019 to October 2019 in HFC IVF Center. We included 50 women with PCOS and 50 healthy controls. Polymorphism of the LHCGR (ins18LQ) gene was genotyped using polymerase chain reaction-restriction fragment length polymorphism.
RESULTS: From this study, we found that there was no significant difference in the proportion of ages between the groups (p > 0.05). There were significant differences in the characteristics of body mass index, FSH level, LH level, and LH/FSH ratio between the PCOS and control groups (p < 0.05). We also found that the proportion of heterozygote variant non-ins/ins was higher in the PCOS group compared to the control group, but there was no significant difference between the polymorphisms of the non-ins and non-nonins variants between the PCOS and control groups (p = 0.269). The frequency of ins alleles was higher in the PCOS group compared to the control group.
CONCLUSION: There was no significant association between LHCGR ins18LQ gene polymorphism and PCOS.
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References
Speroff L, Fritz AM. Clinical Gynecologic Endocrinology and Infertility: Chronic Anovulation and the Polycystic Ovary Syndrome. 8th ed. North Caroline: Lippincott Williams and Wilkins; 2011. p. 495-531.
Bassiouny YA, Rabie WA, Hassan AA, Darwish RK. Association of the luteinizing hormone/choriogonadotropin receptor gene polymorphism with polycystic ovary syndrome. Gynecol Endocrinol. 2014;30(6):428-30. https://doi.org/10.3109/095135 90.2014.895982 PMid:30182769
Elshal AS, Zidan HE, Rashad NM, Abdelaziz AM, Harira MM. Association between genes encodingcomponents of the leutinizing hormone/luteinizing hormone– choriogonadotrophinreceptor pathway and polycystic ovary syndrome in Egyptian women. Int Union Biochem Mol Biol. 2015;68(1):23-36. https://doi.org/10.1002/iub.1457 PMid:26662070
Panda PK, Rane R, Ravichandran R, Singh S, Panchal H. Genetics of PCOS: A systematic bioinformatics approach to unveil the proteins responsible for PCOS. Genom Data. 2016;8:52-60. https://doi.org/10.1016/j.gdata.2016.03.008
Robeva R, Andonova S, Tomova A, Kumanov P. LHCG receptor polymorphisms in PCOS patients. Biotechnol Biotechnol Equipment. 2018;1:1-6. https://doi.org/10.1080/13102818.2017 .1423246
Liaqat I, Jahan N, Krikun G, Taylor HS. Genetic polymorphisms in Pakistani women with polycystic ovary syndrome. Reprod Sci. 2014;22(3):347-57. PMid:25100445
Zou J, Wu D, Liu Y, Tan S. Association of luteinizing hormone/ choriogonadotropin receptor gene polymorphisms with polycystic ovary syndrome risk: A meta-analysis. Gynecol Endocrinol. 2019;35(1):81-5. https://doi.org/10.1080/09513590 .2018.1498834 PMid:30182769
Thathapudi S, Kodati V, Erukkambattu J, Addepally U, Qurratulain H. Association of luteinizing hormone chorionicgonadotropin receptor gene polymorphism (rs2293275) with polycystic ovarian syndrome. Genet Test Mol Biomarkers. 2015;19(3):128-32. https://doi.org/10.1089/gtmb.2014.0249 PMid:25565299
Chen ZJ, Zhao H, He L, Shi Y, Qin Y, Shi, Y, et al. Genome-wide association study identifies susceptibility loci for polycystic ovary syndrome on chromosome 2p16.3, 2p21 and 9q33.3. Nat Genet. 2011;43(1):55-9. https://doi.org/10.1038/ng.732 PMid:21151128
Ha L, Shi Y, Zhao J, Li T, Chen ZJ. Association study between polycystic ovarian syndrome and the susceptibility genes polymorphisms in Hui Chinese women. PLoS One. 2015;10(5):1- 10. https://doi.org/10.1371/journal.pone.0126505 PMid:25978310
Liu N, Ma Y, Wang S, Zhang X, Zhang Q, Zhang X, Fu L, et al. Association of the genetic variants of luteinizing hormone, luteinizing hormone receptor and polycystic ovary syndrome. Reprod Biol Endocrinol. 2012;10:36. https://doi. org/10.1186/1477-7827-10-36 PMid:22546001
Almawi WY, Hubail B, Arekat DZ, Al-Farsi SM, Al-Kindi SK, Arekat MR, et al. Leutinizing hormone/choriogonadotropin receptor and follicle stimulating hormone receptor gene variants in polycystic ovary syndrome. J Assist Reprod Genet. 2015;32(4):607-14. https://doi.org/10.1007/s10815-015-0427-0 PMid:25649397
Crespo RP, Bachega TA, Mendonca BB, Gomes LG. An update of genetic basis of PCOS pathogenesis. Arch Endocrinol Metab. 2018;62(3):352-61. https://doi. org/10.20945/2359-3997000000049 PMid:29972435
Gambineri A, Pelusi C, Vicennati V, Pagotto U, Pasquali R. Obesity and the polycystic ovary syndrome. Int J Obes Relat Metab Disord. 2002;26(7):883-96. https://doi.org/10.1038/ sj.ijo.0801994 PMid:12080440
Valkenburg O, Steegers-Theunissen RP, Smedts HP, Dallinga- Thie GM, Fauser BC, Westerveld FH, et al. A more atherogenic serum lipoprotein profile is present in women with polycystic ovary syndrome: A case control study. J Clin Endocrinol Metab. 2008;93(2):470-6. https://doi.org/10.1210/jc.2007-1756 PMid:18056772
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Copyright (c) 2020 Hilma Putri Lubis, Muhammad Fidel Ganis Siregar, Ichwanul Adenin, Binarwan Halim, Henry Salim Siregar, M. Oky Prabudi (Author)
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