The Effect of Giving Dadih on Malondialdehyde Levels and Renal Interstitial Fibrosis at Aging Kidney
DOI:
https://doi.org/10.3889/oamjms.2020.4285Keywords:
Dadih, Malondialdehyde, Renal interstitial fibrosisAbstract
BACKGROUND: The increase in the elderly poses problems in the areas of health, since it can cause aging of the physiological function of organs. It also has an impact on kidney function, which will enhance the chronic kidney disease. One of the theory that causes aging process is the free radical theory, which that accumulation of free radicals is caused by depletion of antioxidants. Therefore, exogenous antioxidants are needed and in this study author use dadih. Dadih is fermented buffalo milk, indigenous from West Sumatera-Indonesia. Peptides found in fermented milk contained antioxidant that can stimulate the formation of endogenous antioxidants and diversification of dadiah increases its effect as antioxidant.
AIM: This study aims to prove the effect of dadih antioxidants on malondialdehyde (MDA) levels of kidney tissue and renal interstitial fibrosis.
MATERIALS AND METHODS: An experimental study with post-test only control group design conducted to observe effect of dadih to kidney aging of Rattus norvegicus in the Laboratory of Andalas University. It used 30 R. norvegicus which were divided into three groups. Group K was a positive control (did not get dadih), Group P1 was given dadih 4.5 g once a day, and Group P2 was given dadih 4.5 g twice a day for 42 days. After that, MDA levels of kidney tissue are examined using the thiobarbituric acid reactive substances examination technique and examination of renal interstitial fibrosis which is done by histopathology with Sirius-red staining. Data were analyzed using the normality test with Shapiro–Wilk.
RESULTS: The results showed that dadih can reduce levels of MDA in kidney tissue, where its decrease very significant in Group P1 (given dadih once a day) and Group P2 (given dadih twice a day) compared to the Group K (control group) 0.97 ± 0.06 pg/ml to 0.75 ± 0.03 pg/ml (p < 0.05). Group P1 and Group P2 can also reduce the renal interstitial fibrosis rank. Obtained a decrease in the average fibrosis rank from Group K to Group P1 and subsequently to Group P2 (p < 0.05).
CONCLUSION: This study concluded that dadih can reduce MDA levels in kidney tissue and reduce renal interstitial fibrosis rank on aging kidney.
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References
Campbell KH, O’Hare AM. disease in the elderly: Update on recent literature. Curr Opin Nephrol Hypertens. 2008;17(3):298-303. PMid:18408482
Goldsmith TC. Modern evolutionary mechanics theories and resolving the programmed/non-programmed aging controversy. Biochemistry (Mosc). 2014;79(10):1049-55. https://doi. org/10.1134/s000629791410006x PMid:25519063
Stuart JA, Maddalena LA, Merilovich M, Robb EL. A midlife crisis for the mitochondrial free radical theory of aging. Longev Healthspan. 2014;3:1-15. https://doi. org/10.1186/2046-2395-3-4 PMid:24690218
Small DM, Coombes JS, Johnson B, Gobe GC. Oxidative stress, anti-oxidant therapies and chronic kidney disease. Nephrology. 2012;17(4):311-21. https://doi. org/10.1111/j.1440-1797.2012.01572.x PMid:22288610
Li G, Chen Y, Hu H, Liu L, Hu X. Association between age-related decline of kidney function and plasma malondialdehyde. Rejuvenation Res. 2012;15(3):257-67. https://doi.org/10.1089/ rej.2011.1259 PMid:22530729
Frijhoff J, Winyard PG, Zarkovic N, Davies SS, Stocker R, Cheng D, et al. Clinical relevance of biomarkers of oxidative stress. Antioxid Redox Signal. 2015;23(14):1144-70. https://doi. org/10.1089/ars.2015.6317 PMid:26415143
Lan H Y. Diverse roles of TGF-β/smads in renal fibrosis and inflammation. Int J Biol Sci. 2011;7(7):1056-67. https://doi. org/10.7150/ijbs.7.1056 PMid:21927575
Clare, DA, Swaigood, HE. Bioactive milk peptides: A prospectus. J Dairy Sci. 2000;83(6):1187-95. PMid:10877382
Korhonen H, Pihlanto A. Bioactive peptides: Production and functionality. Int Dairy J. 2006;16:945-60. https://doi. org/10.1016/j.idairyj.2005.10.012
Rusfidra PE, Juliyarsi AI, Purwanto H. Plasma Nutfah Sumatera Barat Dadiah Sebagai Pangan Fungsional Probiotik Menunjang Kesehatan Masyarakat. Bogor: Cendikia; 2010. https://doi. org/10.25077/car.9.9
Shen Q, Shang N, Li P. In vitro and in vivo antioxidant activity of Bifidobacterium animalis 01 isolated from centenarians. Curr Microbiol. 2011;62(4):1097-103. https://doi.org/10.1007/ s00284-010-9827-7 PMid:21132298
Persichetti E, Chiasserini D, Parnetti L, Eusebi P, Paciotti S, Carlo CD, et al. Factors influencing the measurement of lysosomal enzymes activity in human cerebrospinal fluid. PLoS One. 2014;9(7):e101453. https://doi.org/10.1371/journal. pone.0101453 PMid:24983953
Wang AN, Yi XW, Yu HF, Dong B, Qiao SY. Free radical scavenging activity of Lactobacillus fermentum in vitro and its antioxidative effect on growing-finishing pigs. J Appl Microbiol. 2009;107(4):1140-8. https://doi. org/10.1111/j.1365-2672.2009.04294.x PMid:19486423
Nardone A, Ronchi B, Lacetera N, Ranieri MS, Bernabucci U. Effects of climate changes on animal production and sustainability of livestock system. Livest Sci. 2010;130(1):57-69. https://doi.org/10.1016/j.livsci.2010.02.011
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Copyright (c) 2020 Harnavi Harun, Yanwirasti Yanwirasti, Bambang Purwanto, Endang Purwati Rahayuningsih (Author)
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