Cyclea barbata Miers Ethanol Extract and Coclaurine Induce Estrogen Receptor α in the Development of Follicle Pre-ovulation

Noviyanti Noviyanti; Yuyun Yueniwati; Mulyohadi Ali; Bambang Rahardjo; Galuh W Permatasari

doi:10.3889/oamjms.2020.4418

Authors

Noviyanti Noviyanti Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia; Midwifery Program, School of Health Sciences Jenderal Achmad Yani Cimahi, West Java, Indonesia
Yuyun Yueniwati Department of Radiology, Public Hospital of Saiful Anwar, Malang, Indonesia
Mulyohadi Ali Department of Pharmacology, Universitas Brawijaya, Malang, Indonesia; Department of Medical, Universitas Brawijaya, Malang, Indonesia
Bambang Rahardjo Department of Obstetric Gynecology, Public Hospital of Saiful Anwar, Malang, Indonesia
Galuh W Permatasari Indonesian Research Institute for Biotechnology and Bioindustry (IRIBB), Bogor, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2020.4418

Keywords:

Coclaurine, Cyclea barbata, ESRα, Molecular docking, Follicle pre-ovulation

Abstract

BACKGROUND: Menstrual disorders to women are generally caused by the disturbance of estrogen production level. This can cause problems in the female reproductive organs. Herbal medications at this moment are demanding to prevent the risk of chemical substances, one of them is grass jelly leaves (Cyclea barbata) which has the potency to induce estrogen levels to women and some people use it to overcome fertility problems. One of the active compounds content is coclaurine.

AIM: The study aimed to know the main compound leaves of C. barbata which has an important role in estrogen induction, protein target, and pathway through to activate estrogen production.

METHODS: There are 18 active compounds from C. barbata gained from database and had been confirmed the presence of coclaurine compound used liquid chromatography-mass spectrometry. Then, it predicted that the potency of molecular interaction between active compounds of ESRα had been done by the molecular docking and in vitro approach.

RESULTS: Coclaurine has the highest binding affinity to activate ESRα. Even though the network analysis prediction did not show the direct interaction, we predict the coclaurine might first interact with other steroid proteins showing by Cytoscape analysis. In vitro results showing the maximum dosage of C. barbata extract is in 100 ppm, while coclaurine dosage is optimum at 100 and 200 μM/L to induce the maturation of oocytes.

CONCLUSION: Coclaurine compounds give stimulation of hormonal induction through several proteins such as SRC, ADRB2, and ADRB3 which involve CYP1A1 and CYP19A1, then activate estrone and estradiol to help the increase of estrogen. C. barbata ethanol extracts and coclaurine had been proved having ability to enhance development follicles pre-ovulation.

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Author Biography

Mulyohadi Ali, Department of Pharmacology, Universitas Brawijaya, Malang, Indonesia; Department of Medical, Universitas Brawijaya, Malang, Indonesia

Pharmacology Department, Medical Department, Universitas Brawijaya, Malang, Indonesia

References

Wei S, Schmidt MD, Dwyer T, Norman RJ, Venn AJ. Obesity and menstrual irregularity: Associations with SHBG, testosterone, and insulin. Obes (Silver Spring). 2009;17(5):1070-6. https:// doi.org/10.1038/oby.2008.641 PMid:19180069

Hamilton PM. Dasar-dasar Keperawatan Maternitas. 6th ed. Jakarta: EGC; 2014.

Samardzija D, Pogrmic-Majkic K, Fa S, Glisic B, Stanic B, Andric N. Atrazine blocks ovulation via suppression of Lhr and Cyp19a1 mRNA and estradiol secretion in immature gonadotropin-treated rats. Reprod Toxicol. 2016;61:10-8. https://doi.org/10.1016/j.reprotox.2016.02.009 PMid:26923738

Fritz MA, Speroff L. Clinical Gynecologic Endocrinology and Infertility. 8th ed. Philadelphia, PA: LWW; 2011.

Tang H, Liu Y, Li J, Li G, Chen Y, Yin Y, et al. 2017. LH signaling induced ptgs2a expression is required for ovulation in zebrafish. Mol Cell Endocrinol. 2017;447:125-33. https://doi.org/10.1016/j. mce.2017.02.042 PMid:28254490

Santo AP, Susilo U, Wijayanti GE. Perkembangan oosit induk (Osteochilus hasseltii) CV yang diberi hormon estradiol 17β dan pakan dengan kadar protein berbeda. Scr Biol. 2014;1:35-44. https://doi.org/10.20884/1.sb.2014.1.1.23

Shodiq AM. Uji Aktivitas Antioksidan Ekstrak dan Fraksi daun Cincau Hijau Rambat (Cyclea barbata Miers) dan Identifikasi Golongan Senyawa dari Fraksi Yang Palig Aktif. Indonesia: Universitas Indonesia; 2012. https://doi.org/10.18860/ al.v0i0.2895

Widiyati E. Penentuan adanya senyawa triterpenoid dan uji aktivitas biologis pada beberapa spesies tanaman obat tradisional masyarakat pedesaan Bengkulu. GRADIEN 2006;2:116-22.

Harborne. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Bandung: Institut Teknologi Bandung; 2006.

Cahyati Y, Santoso DR, Juswono UP. Effects of radiation on decreased estrogen and consumption of isoflavones to prevent early menopause in radiation therapy. Nat B Univ Brawijaya Malang 2013;2(2):109-15. https://doi.org/10.21776/ ub.natural-b.2013.002.02.2

Afendi FM, Okada T, Yamazaki M, Hirai-Morita A, Nakamura Y, Nakamura K, et al. KNApSAcK family databases: Integrated metabolite-plant species databases for multifaceted plant research. Plant Cell Physiol. 2012;53(2):e1. https://doi. org/10.1093/pcp/pcr165 PMid:22123792

Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, Weissig H, et al. The protein data bank. Nucleic Acids Res. 2000;28(1):235-42. PMid:10592235

Trott O, Olson AJ. AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading. J Comput Chem. 2010;31(2):455-61. https://doi.org/10.1002/jcc.21334 PMid:19499576

Kuhn M, von Mering C, Campillos M, Jensen LJ, Bork P. STITCH: Interaction networks of chemicals and proteins. Nucleic Acids Res. 2008;36(Database issue):D684-8. https://doi.org/10.1093/ nar/gkm795 PMid:18084021

Malo N, Hanley JA, Cerquozzi S, Pelletier J, Nadon R. Statistical practice in high-throughput screening data analysis. Nat Biotechnol. 2006;24(2):167-75. https://doi.org/10.1038/nbt1186 PMid:16465162

Anita A, Kurniani KN, Agus SM. Developmental competence of maturation and fertilization prepubertal sheep oocytes in vitro. J Vet. 2017;18(1):51-8. https://doi.org/10.19087/ jveteriner.2017.18.1.51

Ciptadi G, Susilawati T, Siswanto B, Karima HN. The efficiency of gonadotrophinb supplementation of mSOF medium on the maturation rate of immature oocyte in vitro. J Ternak Trop. 2012;12;108-14.

Kiptiyah K, Widodo W, Ciptadi G, Aulanni’am A, Widodo MA, Sumitro SB. 10-Gingerol as an inducer of apoptosis through HTR1A in cumulus cells: In-vitro and in-silico studies. J Taibah Univ Med Sci 2017;12(5):397-406. https://doi.org/10.1016/j. jtumed.2017.05.012

Gibb W, Lye SJ, Challis JR. Parturition. In: Neill JD, editor. Physiology of Reproduction. Cambridge, Massachusetts: Academic Press; 2006. p. 2925-74. https://doi.org/10.1016/ b978-012515400-0/50060-9

Pepe GJ, Albrecht ED. Actions of placental and fetal adrenal steroid hormones in primate pregnancy. Endocr Rev. 1995;16(5):608-48. https://doi.org/10.1210/er.16.5.608 PMid:8529574

Vrtačnik P, Ostanek B, Mencej-Bedrač S, Marc J. The many faces of estrogen signaling. Biochem Med (Zagreb). 2014;24(3):329- 42. https://doi.org/10.11613/bm.2014.035 PMid:25351351

Syarif RA, Kadarsih SS, Meiyanto E, Wahyuningsih MS. Effects of curcumin on estrogen secretion and estrogen receptor β in pig granulosa cells of medium follicles. J Kedokt Brawijaya. 2016;29:32-8.

Sánchez F, Smitz J. Molecular control of oogenesis. Biochim Biophys Acta. 2012;1822(12):1896-912. https://doi. org/10.1016/j.bbadis.2012.05.013 PMid:22634430

Richards JS, Russell DL, Ochsner S, Hsieh M, Doyle KH, Falender AE, et al. Novel signaling pathways that control ovarian follicular development, ovulation, and luteinization. Recent Prog Horm Res. 2002;57:195-220. https://doi.org/10.1210/ rp.57.1.195 PMid:12017544