The Effect of Giving Dadih on Manganese Superoxide Dismutase Gene Expression and Malondialdehyde Levels at Aging Kidney

Harnavi  Harun; Yanwirasti  Yanwirasti; Bambang  Purwanto; Endang Purwati  Rahayuningsih

doi:10.3889/oamjms.2020.4454

Authors

Harnavi Harun Department of Internal Medicine, Faculty of Medicine, Andalas University, Dr M Djamil Central General Hospital Padang, Indonesia; Department of Anatomy, Faculty of Medicine, Andalas University, Padang, Indonesia
Yanwirasti Yanwirasti Department of Internal Medicine, Faculty of Medicine, Sebelas Maret University, Solo, Indonesia
Bambang Purwanto Department of Biotechnology, Faculty of Animal Husbandary, Andalas University, Padang Indonesia
Endang Purwati Rahayuningsih Faculty of Animal Husbandry, Andalas University, Padang, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2020.4454

Keywords:

Dadih, Manganese superoxide dismutase, Malondialdehyde

Abstract

BACKGROUND: The aging process is a complex physiological mechanism that cannot be disclosed in exact detail. Various theories about the aging process have been put forward by many experts.

AIM: This study aims to prove the effect of dadih on manganese superoxide dismutase (MnSOD) gene expression and malondialdehyde levels at aging kidney.

MATERIALS AND METHODS: This study used 30 Rattus norvegicus which were divided into three groups: Group 1 was a positive control (did not get dadih), Group 2 received dadih 1 × 4.5 g/day, and Group 3 received dadih 2 × 4.5 g/day for 42 days. After that, MDA levels of kidney tissue are examined using the thiobarbituric acid reactive substances examination technique and examination of kidney and examination of MnSOD was done by immunohistochemical staining of kidney tissue. Data were analyzed using the normality test with Shapiro-Wilk.

RESULTS: The results showed that dadih were be able to decreased MDA levels in Group 2 and Group 3 compared to Group 1 (control) 0.97 ± 0.06 pg/mL to 0.75 ± 0.03 (p < 0.05). Dadih also increased MnSOD expression sequentially from Group 1 33.66 ± 9.29 to Group 2 53.58 ± 8.51 and Group 3 73.70 ± 4.29 (p < 0.05)

CONCLUSION: This study concluded that dadih can reduce MDA levels in old kidney tissue and dadih can increase the expression of MnSOD.

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