The Effect of Ethanol and Ethyl Acetate Fraction of Chayote fruit (Sechium edule Jacq. Swartz) on the Oxidative Stress and Insulin Resistance of Male White Rat Model Type 2 Diabetes Mellitus

Authors

  • Jekson Martiar Siahaan Doctoral Program, Universitas Sumatera Utara, Medan, Indonesia; Department of Physiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Syaffruddin Illyas Department of Biology, Faculty of Mathematics and Natural Science, Universitas Sumatera Utara, Medan, Indonesia
  • Dharma Lindarto Department of Internal Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Marline Nainggolan Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2020.4517

Keywords:

Type 2 DM, Insulin Resistance, Streptozotocin, Nicotinamide, High-fat diet (HFD)

Abstract

BACKGROUND: Oxidative stress in type 2 diabetes mellitus (T2D) causes insulin resistance and disordered insulin secretion. Pathomechanisms of T2D consist of dysfunctional pancreatic β-cell and insulin resistance caused by free radical (reactive oxygen species and reactive nitrogen species) that produced from the glucose metabolism pathway. Insulin resistance can be measured using the homeostatic model assessment of insulin resistance (HOMA-IR). Oxidative stress can measure through the activities of malondialdehyde (MDA) and superoxide dismutase (SOD).

AIM: This research aims to study the potential of chayote (Sechium edule Jacq. Swartz) to be used as antihyperglycemic in T2D.

MATERIALS AND METHODS: This research was conducted with a post-test randomized controlled group design. Eleven groups with four male rats each were used. Normal untreated rats were treated under ad libitum feeding and drinking condition. Meanwhile, the rat models were induced with the combination of 45 mg/kg b.w. streptozotocin, 110 mg/kg b.w. nicotinamide, 40.5 mg/kg b.w. metformin, high-fat diet, and/or chayote extract. The chayote extract was orally administered to the rat in the form of ethanol extract and/or ethyl acetate fraction, with three dosages of 45 mg/kg b.w., 100 mg/kg b.w., and 150 mg/kg b.w. for each extract type. The body weight, glucose level, insulin level, MDA, and SOD activities were measured. The HOMA-IR was used.

RESULTS: The lowest body weight of the rat model in week 0 was 145 ± 25.31, founded in Group H that was treated with ethyl acetate fraction of chayote extract (45 mg/kg b.w.). The lowest blood sugar level in the group with 2 h glucose load was 112.5 ± 27.00 on average, found in Group G that was treated with chayote ethanolic extract (150 mg/kg b.w.). The highest SOD in the group treated with chayote extract was 1.27 ± 0.20, founded in Group H treated with ethyl acetate 45 mg/kg b.w. The lowest level of MDA was 0.86 ± 0.70 in Group H treated with ethyl acetate 45 mg/kg b.w. The lowest fasting blood sugar spectrophotometer level was 150.54 ± 17.24 mg/dl in Group K with metformin treatment, followed by 155.16 ± 31.92 mg/dl in Group K treated 45 mg/kg b.w. ethanol treatment. The highest insulin level was 6.14 ± 0.71, founded in Group F that was treated with chayote ethanolic extract 100 mg/kg b.w. The lowest measurement of HOMA-IR was 0.16 ± 0.80 in Group E treated with ethanol extract of chayote 45 mg/kg b.w.

CONCLUSION: Ethanol extract and fractionation of chayote work as an antioxidant and anti-insulin resistance.

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References

Sheikhpour R. Diabetes and oxidative stress: The mechanism and action. Iran J Diabetes Obes. 2013;5(1):40-5.

Ganjifrockwala FA, Joseph JT, George G. Decreased total antioxidant levels and increased oxidative stress in South African Type 2 diabetes mellitus patients. J Endocrinol Metab Diabetes South Afr. 2017;22(2):21-5. https://doi.org/10.1080/16089677.2017.1324590

Tangvarasittichai S. Oxidative stress, insulin resistance, dyslipidemia and Type 2 diabetes mellitus. World J Diabetes. 2015;6(3):456-80. https://doi.org/10.4239/wjd.v6.i3.456 PMid:25897356

Holt RI. Diagnosis, epidemiology and pathogenesis of diabetes mellitus: An update for psychiatrists. Br J Psychiatry. 2004;47:S55-63. https://doi.org/10.1192/bjp.184.47.s55 PMid:15056594

Wallace TM, Levy JC, Matthews DR. Use and abuse of HOMA modeling. Diabetes Care. 2004;27(6):1487-95. https://doi.org/10.2337/diacare.27.6.1487 PMid:15161807

Popa S, Mota M. Beta-cell Function and Failure in Type 2 Diabetes. London, United Kingdom: IntechOpen; 2013.

Kaku K. Pathophysiology of Type 2 DM and its treatment policy. JMAJ. 2010;53(1):41-6.

Ozougwu JC, Obimba KC, Belonwu CD, Unakalamba CB. The pathogenesis and pathophysiology of Type 1 and Type 2 diabetes mellitus. Acad J. 2013;4(4):46-57.

Stauffacher W, Burr I, Gutzeit A, Beaven D, Veleminsky J, Renold AE. Streptozotocin diabetes: Time course of irreversible B-cell damage; further observations on prevention by nicotinamide. Exp Biol Med. 1970;133(1):194-200. https://doi.org/10.3181/00379727-133-34439

Szkudelski T. Streptozotocin-nicotinamide-induced diabetes in the rat. Characteristics of the experimental model. Exp Biol Med. 2012;237(5):481-90. https://doi.org/10.1258/ebm.2012.011372 PMid:22619373

Siahaan JM, Harahap U, Loesnihari R. Effect of ethanol extract of chayote (Sechium edule Jacq. Swartz) on the activity of glutathione peroxide (GPx) in house mice (Mus musculus L) strain DD webster hyperglycemia induced by streptozotocin (STZ). Indones J Med. 2016;1(1):44-9. https://doi.org/10.26911/theijmed.2016.01.01.06

Siahaan, JM. Effect of antihipoglycemic Sechium edule Jacq. Swartz. Etanol extract on histopathologic changes in hyperglycemic Mus musculus L. Indones J Med. 2017;2(2):86- 93. https://doi.org/10.26911/theijmed.2017.02.02.02

Pari L, Karthikesan K, Menon VP. Comparative and combined effect of chlorogenic acid and tetrahydrocurcumin on antioxidant disparities in chemical induced experimental diabetes. Mol Cell Biochem. 2010;341(1-2):109-17. https://doi.org/10.1007/s11010-010-0442-5 PMid:20339905

Firdous SM, Singh A. Effect of Ipomoea staphylina leaves on streptozotocin-nicotinamide induced Type-II diabetes in Wistar. Asian Pac J Health Sci. 2016;3(3):30-44. https://doi.org/10.21276/apjhs.2016.3.3.6

Listianasari Y, Dirgahayu P, Wasita B, Nuhriawangsa A, Magna P. Efektivitas pemberian jus labu siam (Sechium edule) terhadap profil lipid tikus (Rattus novergicus) model hiperlipidemia. Penelitian Gizi Makanan. 2017;40(1):35-43. https://doi.org/10.22435/pgm.v40i1.6046.35-43

Leite AC, Araujo TG, Carvalho BM, Maia MB, Lima VL. Characterization of the antidiabetic role of Parkinsonia aculeata (Caesalpineaceae). Evid Based Complement Alternat Med. 2011;9:692378. https://doi.org/10.1155/2011/692378

Sornalakshmi V, Tresina SP, Paulpriya K, Packia LM, Mohan VR. Oral glucose tolerance test (OGTT) in normal control and glucose induced hyperglycemic rats with Hedyotis leschenaultiana DC. Int J Toxicol Pharmacol Res. 2016;8(1):59- 62. https://doi.org/10.1201/9780429487767-22

Sulaiman CT, Shahida V, Balachandran I. Effect of extraction solvent on the phytoconstituents of Aegle marmelos (L.) correa. J Natl Remedies. 2015;15(1):58-64. https://doi.org/10.18311/jnr/2015/498

Olaokun OO, McGaw LJ, Rensburg IJ, Jacobus N, Eloff JN, Naidoo V. Antidiabetic activity of the ethyl acetate fraction of Ficus lutea (Moraceae) leaf extract: Comparison of an in vitro assay with an in vivo obese mouse model. BMC Complement Altern Med. 2016;16:110. https://doi.org/10.1186/s12906-016-1087-z PMid:27029351

Balasubramanian T, Chatterjee TK, Senthilkumar GP, Mani T. Effect of potent ethyl acetate fraction of Stereospermum suaveolens extract in streptozotocin-induced diabetic rats. ScientificWorldJournal. 2012;2012:413196. https://doi.org/10.1100/2012/413196 PMid:22593683

Kumarappan CT, Thilagam E, Vijayakumar M, Mandal SC. Modulatory effect of polyphenolic extracts of Ichnocarpus frutescens on oxidative stress in rats with experimentally induced diabetes. Indian J Med Res. 2012;136(5):815-21. https://doi.org/10.1016/j.sjbs.2012.04.004 PMid:23287129

Verma N, Amresh G, Sahu PK, Rao CV, Singh AP. Antihyperglycemic and antihyperlipidemic activity of ethyl acetate fraction of Rhododendron arboreum smith flowers in streptozotocin induced diabetic rats and its role in regulating carbohydrate metabolism. Asian Pac J Trop Biomed. 2012;2(9):696-701. https://doi.org/10.1016/s2221-1691(12)60212-3 PMid:23569997

Okoduwa SI, Umar IA, James DB, Inuwa HM. Anti-diabetic potential of Ocimum gratissimum Leaf fractions in fortified diet-fed streptozotocin treated rat model of Type-2 diabetes. Medicines (Basel). 2017;4(4):73. https://doi.org/10.3390/medicines4040073 PMid:29019956

Mousavi L, Mohd SR, Murugaiyah V, Asmawi MZ. Hypoglycemic and anti-hyperglycemic study of Ocimum tenuiflorum L. Leaves extract in normal and streptozotocin-induced diabetic rats. Asian Pac J Trop Biomed. 2016;6(12):1029-36. https://doi.org/10.1016/j.apjtb.2016.10.002

Mhya DH, Anigo KM, Umar IA, Alegbejo JO. Evaluation of anti-hyperglycemic effect of sub-fractions derived from ethanol-aqueous fraction of Balanites aegyptiaca leaves in streptozotocin-induced diabetic rats. Asian J Biol Sci. 2019;12(2):164-72. https://doi.org/10.3923/ajbs.2019.164.172

Hossain MS, Khan MR, Anisuzzaman AS, Ahmed M, Amran MS, Islam A. Antidiabetic and glycogenesis effects of different fractions of ethanolic extract of leaves of Mangifera indica (Linn.) in normal and alloxan induced diabetic rats. J Med Sci. 2010;10:80-6. https://doi.org/10.3923/jms.2010.80.86

Aboonabi A, Rahmat A, Othman F. Antioxidant effect of pomegranate against streptozotocin-nicotinamide generated oxidative stress induced diabetic rats. Toxicol Rep. 2014;1:915- 22. https://doi.org/10.1016/j.toxrep.2014.10.022 PMid:28962304

Ononamadu CJ, Alhassan AJ, Ibrahim A, Imam AA, Ihegboro GO, Owolarafe TA, et al. Methanol-extract/fractions of Dacryodes edulis Leaves ameliorate hyperglycemia and associated oxidative stress in streptozotocin-induced diabetic Winstar rats. J Evid Based Integr Med. 2019;24:1-12. https://doi.org/10.1177/2515690x19843832 PMid:31055945

Ubhenin B, Adamude FA, Dingwoke EJ, Nwobodo NN. Hypolipidamic study of ethyl acetate leaves extract of Momordica charantia on the biochemical parameters of streptozotocin-nicotinamide induced Type 2 diabetic rats. Biomed Res. 2019;30(4):201. https://doi.org/10.35841/biomedicalresearch.30-19-201

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Published

2020-12-15

How to Cite

1.
Siahaan JM, Illyas S, Lindarto D, Nainggolan M. The Effect of Ethanol and Ethyl Acetate Fraction of Chayote fruit (Sechium edule Jacq. Swartz) on the Oxidative Stress and Insulin Resistance of Male White Rat Model Type 2 Diabetes Mellitus. Open Access Maced J Med Sci [Internet]. 2020 Dec. 15 [cited 2022 Dec. 7];8(A):962-9. Available from: https://oamjms.eu/index.php/mjms/article/view/4517

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