Toxicological and Biological Assessment of the Effect of Sapropel of the Prybych Lake during Cutaneous Application in Rats

Authors

  • Oksana Strus Department of Drug Technology and Biopharmaceutics, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
  • Konstantin Babov Department of Medical Rehabilitation, State Institution “Ukrainian Research Institute of Medical Rehabilitation Therapy of Ministry of Health of Ukraine,” Odessa, Ukraine
  • Sergey Gushcha Department of Medical Rehabilitation, State Institution “Ukrainian Research Institute of Medical Rehabilitation Therapy of Ministry of Health of Ukraine,” Odessa, Ukraine
  • Nataliia Polovko Department of Drug Technology, National University of Pharmacy, Kharkiv, Ukraine
  • Boris Nasibullin Department of Basic Research, State Institution “Ukrainian Research Institute of Medical Rehabilitation Therapy of Ministry of Health of Ukraine,” Odessa, Ukraine
  • Tetiana Polshchakova Department of Medical Rehabilitation, State Institution “Ukrainian Research Institute of Medical Rehabilitation Therapy of Ministry of Health of Ukraine,” Odessa, Ukraine

DOI:

https://doi.org/10.3889/oamjms.2020.4743

Keywords:

Sapropels silt deposits, Acute toxicity, Local irritating, Sensitizing effect

Abstract

BACKGROUND: The use of sapropel in balneology, cosmetology, and also products of its processing as an active pharmaceutical ingredient for the creation of medicines requires a mandatory assessment of the degree of toxicity. The toxicological and biological characteristics of sapropel (silt deposits) from the Prybych lake of the Shatsky Lakes group of the Volyn region of Ukraine were based on the results of studies of the functional state of the central nervous system (CNS), autonomic reactions, and structural and functional changes in the internal organs (heart, stomach, liver, and kidneys) of white rats with external use of sapropel. An analysis of acute toxicity, locally irritating, and sensitizing action of sapropaste (a product from sapropel) was also carried out.

AIM: This study aimed to conduct of toxicological and biological assessment of the effect of sapropel from the Prybych Lake during cutaneous application in rats to justify the prospects for its further use in balneology, medicine, and for the development of new drugs.

METHODS: This study was an in vivo experimental study. As many as, 50 rats were grouped into three treatment groups and two groups of controls.

RESULTS: In essence, indicators of locomotor activity and orientation-research behavior of animals do not change (p > 0.5). A stimulating effect on the emotional state of animals, characterized by an increase of grooming duration (p < 0.001), was found. Animals feel calm. A moderate stimulating effect on vegetative reactions manifests itself in a slight increase in urination acts (p < 0.05). Microscopic studies confirmed the absence of the damaging effect of sapropels on the structural and functional state of the organs studied, and signs of increased secretory activity of the gastric mucosa were also noted.

CONCLUSIONS: It was experimentally established that the sapropel of the Prybych Lake does not have a harmful or toxic effect on the CNSs functional state and the liver, kidneys, heart, and stomach of rats. Sapropaste does not exhibit sensitizing and locally irritating properties and belongs to the class of practically non-toxic substances (toxicity Class V).

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References

Kala KJ, Prashob РK, Chandramohanakumar N. Humic substances as a potent biomaterials for therapeutic and drug delivery system-a review. Int J Appl Pharm. 2019;11(3):1-4.

Peña-Méndez EM, Havel J, Patočka J. Humic substances-compounds of still unknown structure: Applications in agriculture, industry, environment, and biomedicine. J Appl Biomed. 2005;3:13-24. https://doi.org/10.32725/jab.2005.002

Ozkan А, Sen HM, Sehitoglu I, Alacam Н, Guven М, Aras AB, et al. Neuroprotective effect of humic acid on focal cerebral ischemia injury: An experimental study in rats. Inflammation. 2015;38(1):32-9. https://doi.org/10.1007/s10753-014-0005-0 PMid:25173888

Kodama H, Denso, Okazaki F, Ishida S. Protective effect of humus extract against Trypanosoma brucei infection in mice. J Vet Med Sci. 2008;70(11):1185-90. https://doi.org/10.1292/ jvms.70.1185 PMid:19057136

Vucskits AV, Hullár I, Bersényi A, Andrásofszky E, Kulcsár M, Szabó J. Effect of fulvic and humic acids on performance, immune response and thyroid function in rats. J Anim Physiol Anim Nutr (Berl). 2010;94(6):721-8. https://doi. org/10.1111/j.1439-0396.2010.01023.x PMid:21073632

Krempaská K, Vaško L, Vašková J. Humic acids as therapeutic compounds in lead intoxication. Curr Clin Pharmacol. 2016;11(3):159-67. https://doi.org/10.2174/1574884711666160 813233225 PMid:27526696

Bolbatovski GN, Mazur NV, Pirogova LA. Innovative Technologies in the Use of Natural Therapeutic Mud. Medical News; 2014.

Espejo-Antúnez L, Cardero-Durán MA, Garrido-Ardila EM, Torres-Piles SB. Clinical effectiveness of mud pack therapy in knee osteoarthritis. Rheumatology (Oxford). 2013;52(4):659-68. https://doi.org/10.1093/rheumatology/kes322 PMid:23236190

Efendieva FM, Aliev AA, Balakisheva FK, Nabiev NN, Salmanov MM. The Transdermal Application of Dry Knoll Therapeutic Mud in the Practice of Non-spa Treatment. Physiotherapy Balneology and Rehabilitation; 2013.

Gushcha SG, Nasibullin BA, Plakida AL, Volyanskaya VS. Physiological mechanisms of influence of table and treatment mineral waters based on the balgeneological measure magnesium oil on some systems of health organism in experiment. Int J Mol Biol Open Access. 2018;3(3):100-1. https://doi.org/10.15406/ijmboa.2018.03.00059

Badyal DK, Desai С. Animal use in pharmacology education and research: The changing scenario. Indian J Pharmacol. 2014;46(3):257-65. https://doi.org/10.4103/0253-7613.132153 PMid:24987170

Gudz NI, Kalynyuk TG, Bilous SB, Smetanina KI. Good Pharmacy Practices: A Workshop for Graduate Students. Ukraine: Nova Knyga; 2013. p. 368.

Nikipelova OM, Solodova LB. A Guide to the Methods of Controlling Peloids and their Preparations. Part 1. Physico-chemical Research. Odesa, Ukraine: Ministry of Health of Ukraine, Research Institute of Medical Rehabilitation Therapy; 2008. p. 100.

Strus OY, Geyderikh OG, Silaeva LF. Тhe Studies on Development of the Composition of Masks with the Sapropel Paste. News of Рharmacy; 2016. https://doi.org/10.24959/ nphj.16.2132

European Communities. Council Directive 86/609/EEC of 24 November 1986 on the Approximation of the Laws, Regulations and Administrative Provisions on the Member States Regarding the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes. Brussels, Belgium: European Communities; 1986. p. 1-29. https://doi. org/10.5040/9781472559500.0009

National Research Council. Committee for the update of the guide for the care and use of laboratory animals. In: Guide for the Care and Use of Laboratory Animals. 8th ed. Washington, DC: National Academies Press, US; 2011. https://doi. org/10.17226/25801

Walsh RN, Cummings RA. The open-field test: A critical review. Psychol Bull. 1976;83:482-504. PMid:17582919

Lojda Z, Grossrau R, Schiebler TN. Enzyme Histochemistry: A Laboratory Manual. New York: Springer-Verlag Berlin- Heidelberg; 1979.

Ministry of Health of Ukraine. On Approval of the Recommendations of the Research Methods of Biological Effects of Natural Medical Resources and Preformed Medicines. Kyiv: Ministry of Health of Ukraine; 2009. Available from: http://www.old.moz.gov.ua/ua/portal/dn_20090928_692. html. [Last accessed on 2009 Sep 28]. https://doi. org/10.31928/2305-3127-2018.4.3035

Stefanov OV. Preclinical Research of Medicinal Products. Kyiv: Medical Center Avicenna; 2001. p. 528.

Order of the Ministry of Healthcare. Procedure for Conducting Preclinical Study of Drugs and Examination of Materials for Preclinical Study of Drugs. No. 944. Available from: https://www. zakon.rada.gov.ua/laws/show/z005310. [Last accessed on 2009 Dec 14].

Tatem KS, Quinn JL, Phadke А, Yu Q, Gordish-Dressman Н, Nagaraju K. Behavioral and locomotor measurements using an open field activity monitoring system for skeletal muscle diseases. J Vis Exp. 2014;91:51785. https://doi.org/10.3791/51785 PMid:25286313

Gamma TV, Korenyuk II. Effects of bemitil and benzimidazole on behavior of rats in open-field test. Neurophysiology. 2006;38(1):75-80. https://doi.org/10.1007/ s11062-006-0028-8

Sidorov KK. On the Classification of Toxicity of Poisons with Parenteral Routes of Administration. Toxicology of New Industrial Chemicals. Moscow: USSR Academy of Medical Sciences; 1973. p. 47-57.

Berezovskaya IV. Classification of chemicals according to the parameters of acute toxicity with parenteral administration methods. Pharm Chem J. 2003;37(3):32-4.

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Published

2020-09-10

How to Cite

1.
Strus O, Babov K, Gushcha S, Polovko N, Nasibullin B, Polshchakova T. Toxicological and Biological Assessment of the Effect of Sapropel of the Prybych Lake during Cutaneous Application in Rats. Open Access Maced J Med Sci [Internet]. 2020 Sep. 10 [cited 2024 Mar. 28];8(A):633-8. Available from: https://oamjms.eu/index.php/mjms/article/view/4743