The Effect of Follicular-fluid Exposure of Endometriosis Cyst Patients toward the Levels of Bcl-2 and Cytochrome-C in Mice Oocyte

Ida Bagus Putra Adnyana; I Made  Jawi; Ketut Suwiyoga; I Made Bakta; I Nyoman Mantik Astawa; Mochammad Anwar; Ida Bagus Putra  Manuaba; I Wayan Putu  Sutirtayasa; Bagus Komang  Satriyasa

doi:10.3889/oamjms.2020.4791

Authors

Ida Bagus Putra Adnyana Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Udayana, Sanglah General Hospital, Bali, Indonesia
I Made Jawi Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Udayana, Bali, Indonesia
Ketut Suwiyoga Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Udayana, Sanglah General Hospital, Bali, Indonesia
I Made Bakta Department of Internal Medicine, Faculty of Medicine, Universitas Udayana, Sanglah General Hospital, Bali, Indonesia
I Nyoman Mantik Astawa Department of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Udayana, Bali, Indonesia
Mochammad Anwar Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Gajah Mada, Yogyakarta, Indonesia
Ida Bagus Putra Manuaba Department of Chemistry, Faculty of Mathematics and Science, Universitas Udayana, Bali, Indonesia
I Wayan Putu Sutirtayasa Department of Clinical Pathology, Faculty of Medicine, Universitas Udayana, Sanglah General Hospital, Bali, Indonesia
Bagus Komang Satriyasa Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Udayana, Bali, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2020.4791

Keywords:

Bcl-2, Cytochrome C, Endometriosis Cysts, Follicular Fluid

Abstract

BACKGROUND: Endometriosis cysts adversely affect a woman’s quality of life, which causes pain and reduces fertility. The World Health Organization found that the incidence of endometriosis with infertility clinical manifestations is around 10%.

AIM: The purpose of this study is to determine the presence of mice oocytes mitochondrial apoptosis exposed with endometriosis cysts follicular fluid through Bcl-2 and cytochrome C analysis.

METHODS: This study was a randomized post-test only control group design conducted at Sanglah Hospital, Bali Royal Hospital in Denpasar, and the Medical Faculty Udayana University from June 2018 to April 2019. A total of 120 mice oocytes were distributed randomly into three groups, i.e., treatment Groups 1 and 2 that added by 15% endometriosis cysts follicular fluid and control group. Bcl-2 and Cytochrome C analyzed with ELISA Technique. Comparability was tested with one-way ANOVA with a significance level of p < 0.05.

RESULTS: Eighteen mice oocyte replicates were normally distributed and homogeneous. The Bcl-2 levels in the treatment Groups 1, and 2 and control were 627.83 ± 146.42, 634.50 ± 140.62, and 678.83 ± 152.71, respectively; p = 0.838 (not significantly different). Cytochrome C levels in the treatment Groups 1, and 2, and control, respectively, were 3147.75 ± 228.50, 3104.45 ± 211.29, and 2738.28 ± 227.45; p = 0.021 (significantly different).

CONCLUSION: The effect of endometriosis cysts follicular fluid exposure on mitochondrial apoptosis is proven through Cytochrome C, whereas Bcl-2 is not proven.

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