Histological and Clinical Findings in Rabbits Sensitized with GM1 Ganglioside

Ni Komang Sri Dewi Untari; Kurnia  Kusumastuti; Guritno Suryokusumo; I Ketut Sudiana; Tedy Juliandhy

doi:10.3889/oamjms.2020.4871

Authors

Ni Komang Sri Dewi Untari Department of Hyperbaric, Drs. Med. Rijadi S. Phys. Naval Health Institute, Surabaya, Indonesia; Doctoral Program, Faculty of Medicine, Airlangga University, Surabaya, Indonesia
Kurnia Kusumastuti Department of Neurology, Faculty of Medicine, Airlangga University, Surabaya, Indonesia
Guritno Suryokusumo Department of Hyperbaric, Faculty of Medicine, Pembangunan Nasional University, Jakarta, Indonesia
I Ketut Sudiana Department of Pathology Anatomy, Faculty of Medicine, Airlangga University, Surabaya, Indonesia
Tedy Juliandhy Department of Electrical Engineering, Hang Tuah University, Surabaya, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2020.4871

Keywords:

Acute motor axonal neuropathy, Animal model, GM1 ganglioside

Abstract

BACKGROUND: Acute motor axonal neuropathy (AMAN) is a peripheral nerve disorder that attacks motor axons and occurs acutely. AMAN is one type of Guillain–Barre syndrome (GBS) which often attacks men of productive age. Until now, although patients have undergone intravenous immunoglobulin (IVIG) therapy and/or plasmapheresis, long-standing disability remains a problem. In Indonesia, the availability and cost of these therapies are constraints.

AIM: Our study aimed to find a proper animal model suitable for AMAN and can be executed in our institution, Naval Health Institute with a hope to find new therapeutic modalities in healing with AMAN.

METHODS: GM1 ganglioside immunized in New Zealand male white rabbits with complete Freund’s adjuvant, every 3 weeks until 20 weeks. We evaluated the effects GM1 ganglioside on body weight, functional score, and axon degeneration’s scale. Functional score was examined based on Tarlov’s. Hematoxylin-eosin was used to stain this slide.

RESULTS: Rabbits that being immunized with GM1 ganglioside experience a number of neurological signs and symptoms that resemble AMAN, that is, sluggish righting reflex, muscular weakness, flaccid hyper paralysis, and body weight loss. Pathological examination shows extensive degeneration of peripheral nerves, infiltration of macrophages, and perineuritis.

CONCLUSION: This histological and clinical findings support that this neuropathy is induced by an autoimmune response delivered by cells that respond to gangliosides.

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