Analysis of Uncoupling Protein 1 and β3-adrenergic Receptor Single Nucleotide Polymorphisms in Saudi Population with Type 2 Diabetes Mellitus

Fathy М. Elfasakhany; Abdulah M. Karawagh; Shahid S. Siddiqui; Magdi A. Eldamarawi

doi:10.3889/oamjms.2020.4886

Authors

Fathy М. Elfasakhany Department of Basic and Clinical Oral Sciences, Faculty of Dentistry, Umm Al Qura University, Makkah, Saudi Arabia; Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Tanta, Egypt
Abdulah M. Karawagh Department of Internal Medicine, King Abdullah Medical Complex, Jeddah, Saudi Arabia
Shahid S. Siddiqui Department of Basic and Clinical Oral Sciences, Faculty of Dentistry, Umm Al Qura University, Makkah, Saudi Arabia; Department of Medicine, University of Chicago, Chicago, Illinois, USA
Magdi A. Eldamarawi Department of Medical Physiology, Faculty of Medicine, Tanta University, Tanta, Egypt; Department of Medical Physiology, Faculty of Medicine, Tabuk University, Tabuk, Saudi Arabia

DOI:

https://doi.org/10.3889/oamjms.2020.4886

Keywords:

Uncoupling protein type 1, Type 2 diabetes mellitus, β3-adrenergic receptor, Gene polymorphism, Thermogenesis

Abstract

BACKGROUND: Uncoupling proteins (UCP) and β3-adrenergic receptor (ADRB3) gene polymorphism have been implicated in the susceptibility to type 2 diabetes mellitus (T2DM) but the results are inconsistent and inconclusive.

AIM: The aim was to investigate the relationship between -3826A/G (rs1800592) of UCP 1 and ADRB3 Trp64Arg (C/T) (rs4994) gene polymorphism and the incidence of T2DM among Saudis.

METHODS: Genotyping of both UCP1 and ADRB3 genes was carried out in 110 healthy controls and 108 unrelated Saudis with T2DM using polymerase chain reaction-based restriction fragment length polymorphism.

RESULTS: The genotype and allele frequencies of the UCP1 gene did not differ significantly between diabetic subjects and controls (p > 0.05). However, the CT genotype and the T allele of ADRB3 were higher in diabetic subjects compared with the controls while the CC genotype and C allele were higher in the controls relative to the diabetic subjects (Odds ratios [OR]: 7.85, 95% confidence interval [CI]: 4.23–14.59, p < 0.001 and OR: 7.434 95% CI: 4.026–13.39, p < 0.001, respectively).

CONCLUSION: The UCP1 -3826A/G polymorphism may not be associated with the susceptibility to T2DM among Saudis while T allele of ADRB3 may be related to the risk of T2DM, whereas the CC genotype and C allele may confer protection to T2DM.

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