The Effect of Recombinant Human Bone Morphogenetic Protein-7 on the Osteoblast-like Cells Cultured on Implant

Mahmoud Ibrahim Nassar; A. Ahmed; A. Dina; A. Abdullah

doi:10.3889/oamjms.2020.4908

Authors

Mahmoud Ibrahim Nassar Department of Oral Medicine, Oral Diagnosis and Periodontology, Faculty of Dentistry, Aswan University, Tingar, Egypt
A. Ahmed Department of Oral Medicine, Oral Diagnosis and Periodontology, Faculty of Dentistry, Minia University, Minia, Egypt
A. Dina Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt
A. Abdullah Department of Oral Medicine, Oral Diagnosis and Periodontology, Faculty of Dentistry, Minia University, Minia, Egypt

DOI:

https://doi.org/10.3889/oamjms.2020.4908

Keywords:

Osteoclast like cells, implant, Recombinant BMP

Abstract

AIM: The aim of the study was to study the effect of recombinant human bone morphogenetic protein-7 (rhBMP-7) on the osteoblast-like cells cultured on implant.

MATERIALS AND METHODS: The osteoblast-like osteo-1 cell line was used in this experiment and derived from the parietal bone tissue of newborn albino rats. The cells were incubated in a humid atmosphere of 95% air and 5% carbon dioxide at 37°C. The medium was changed every 2 days. Four groups were conducted as follows osteoclast-like cell (machined), implanted osteoclast-like cell on titanium (Ti) (modified), osteoclast-like cell supplemented with BMP-7 (Machined + BMP-7), and implanted osteoclast-like cell on Ti and supplemented with BMP-7 (Modified + BMP-7).

RESULTS: Cell proliferation was influenced by rhBMP-7, as demonstrated by a significant increase in collagen content after 7 and 21 days of culture (p = 0.005) and a significant increase in alkaline phosphatase (ALP) activity after 7 days (p < 0.001). The addition of rhBMP-7 influenced ALP activity, and a significant increase was observed after 21 days (p < 0.001).

CONCLUSION: Within the limitations of the study, we concluded that the presence of rhBMP-7 did not influence cell adhesion to chemically modified Ti surfaces but provided an additional stimulus during the differentiation of rat osteo-1 cells cultured on this type of surface.

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