Experimental Model of Pancreonecrosis Induced by Auto-bile Injection

Grigoriy Abramov; Kairat Shakeyev; Maida Tusupbekova; Ermek Tagaev; Dmitriy Shestakov; Sanzhar Zhumanbaev

doi:10.3889/oamjms.2020.4979

Authors

Grigoriy Abramov Karaganda Medical University, Karaganda, Kazakhstan
Kairat Shakeyev Karaganda Medical University, Karaganda, Kazakhstan
Maida Tusupbekova Karaganda Medical University, Karaganda, Kazakhstan
Ermek Tagaev Karaganda Medical University, Karaganda, Kazakhstan
Dmitriy Shestakov Karaganda Medical University, Karaganda, Kazakhstan
Sanzhar Zhumanbaev Karaganda State Medical University, Karaganda, Kazakhstan

DOI:

https://doi.org/10.3889/oamjms.2020.4979

Keywords:

pancreas, pancreonecrosis, experimental model, auto-bile, acute pancreatitis

Abstract

AIM: The aim of the study is to create an easily reproducible experimental model of pancreonecrosis with the least aggressive technique of the operation.

METHODS: Twenty-two outbred rabbits of comparable weight and age were included in the study. The animals were removed from the experiment 12, 24, and 48 h after the injection of auto-bile into the parenchyma of the pancreas.

RESULTS: After completion of the experiment, rabbit pancreas was extracted, macroscopic and microscopic description was given.

CONCLUSION: According to the results of the study, the proposed model is considered as consistent for reproducing pancreonecrosis in an experiment with less aggressive surgical technique.

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References

Abramov GA, Shakeev KT, Tusupbekova MM, Tagaev EE, Shestakov DV, Zhumanbaev SM. Modeling pancreatic necrosis in an experiment. Certificate on state registration of rights to the subject of copyright No. 7190 2019 dated 12/23/2019, Kazakhstan.

Agapov MA, Gorsky VA, Petrov VA, Polivoda MD, Kravchenko AYu, Battaev AI. Methods of modeling acute pancreatitis (literature review). Bull Russ State Med Univ 2014;3:25-9.

Gushchin YI, Shedko VV, Muzhikyan AA, Makarov MN, Makarov VG. Comparative morphology of the pancreas of experimental animals and humans. Lab Anim Sci Res 2018;3:33-48.

Kirillovyh AS. Morphometric characteristics of the endocrine part of the rodent pancreas in acute destructive pancreatitis. BBC 2011;30:38.

Makarov MN, Rybakova AB, Gushchin YA, Shedko BB, Muzhikyan AA, Makarov VG. Anatomical and physiological characteristics of the digestive tract of humans and laboratory animals. Int Bull Vet Med 2016;1:82-104.

Petrenko VM. The shape and topography of the pancreas. Int J Appl Basic Res 2015;1:1114-7.