The Effect of Lycopene on Cancer Cell Apoptosis by Caspase-9 Concentration Measurement in Indonesian Human Prostate Cancer Cell Culture

Tjahjodjati Tjahjodjati; Suwandi Sugandi; Rainy Umbas; Mieke Satari

doi:10.3889/oamjms.2020.5019

Authors

Tjahjodjati Tjahjodjati Department of Urology, Faculty of Medicine, Padjadjaran University, Dr. Hasan Sadikin General Hospital, Sumedang, Indonesia
Suwandi Sugandi Department of Urology, Faculty of Medicine, Padjadjaran University, Dr. Hasan Sadikin General Hospital, Sumedang, Indonesia
Rainy Umbas Department of Urology, Faculty of Medicine, University of Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia
Mieke Satari Doctoral Program, Faculty of Medicine, Padjadjaran University, Sumedang, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2020.5019

Keywords:

Lycopene, Caspase 9, Prostate cancer

Abstract

BACKGROUND: Lycopene is an antioxidant that mostly found in daily ingredients such as tomatoes. Several studies have shown the lycopene potential in preventing prostate cancer. Nevertheless, the clinical use of lycopene as adjunctive therapy for prostate cancer is still under debate.

AIM: The objective of the study was to determine the effect of lycopene on human prostate cancer cell culture growth by measuring caspase-9 concentration as a marker of the intrinsic pathway of apoptosis in cells.

METHODS: This study was conducted on Indonesian prostate cancer cell culture from a patient with Gleason score 6, divided into 5 subgroups: 2 control groups and 3 treatment groups that were given 1 μM, 2 μM, and 4 μM of lycopene. Measurement of caspase-9 level was performed using enhanced chemiluminescence at 24, 28, and 72 h after lycopene addition in treatment groups. A comparative analysis was performed by two-way ANOVA.

RESULTS: The result showed that there was a significant difference of mean caspase-9 levels in the provision of various concentrations of lycopene and time of observation (p < 0.05). Increased of mean caspase-9 levels started at 2 μM dose of lycopene at 48 h and 4 μM at 24 h (p < 0.05) and continue to rise at 72 h, but caspase-9 was not detected at 1 μM dose in every observation.

CONCLUSION: There was a significant difference of mean caspase-9 levels in the provision of various concentrations of lycopene and time of observation.

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References

Crawford E. Epidemiology of prostate cancer. Urology. 2003;62(6):3-12. https://doi.org/10.1016/j.urology.2003.10.013 PMid:14706503

Sim HG, Cheng CW. Changing demography of prostate cancer in Asia. Eur J Cancer. 2005;41(6):834-45. https://doi.org/10.1016/j.ejca.2004.12.033 PMid:15808953

Antonarakis ES, Carducci MA, Eisenberger MA. Treatment of castration resistant prostate cancer. In: Campbell Walsh Urology 10th ed., Vol. 110. Philadephia, PA: Saunders Elsevier; 2012. p. 2954-70.

Umbas R, Tambunan N. Peran faktor nutrisi pada pencegahan kanker prostat. Indones J Cancer. 2014;8(3):135-40.

Vecchia CL. Mediterranean epidemiological evidence on tomatoes and the prevention of digestive-tract cancers. Proc Soc Exp Biol Med. 1998;218(2):125-8. https://doi.org/10.3181/00379727-218-44276 PMid:9605210

Rao AV. Agarwal S. Review, role of antioxidant lycopene in cancer and heart disease. J Am Coll Nutr. 2000;19(5):563-9. https://doi.org/10.1080/07315724.2000.10718953 PMid:11022869

Basu A, Imrhan V. Tomatoes versus lycopene in oxidative stress and carcinogenesis: Conclusions from clinical trials. Eur J Clin Nutr. 2007;61(3):295-303. https://doi.org/10.1038/sj.ejcn.1602510 PMid:16929242

Giovannucci E. A review of epidemiologic studies of tomatoes, lycopene, and prostate cancer. Exp Biol Med (Maywood). 2002;227(10):852-9. https://doi.org/10.1177/153537020222701003 PMid:12424325

Mills PK, Beeson WL, Phillips RL, Fraser GE. Cohort study of diet, lifestyle, and prostate cancer in adventist men. Cancer. 1989;64(3):598-604. https://doi.org/10.1002/1097-0142(19890801)64:3<598::aid-cncr2820640306>3.0.co;2-6 PMid:2743254

Hadley CW, Miller EC, Schwartz SJ, Clinton SK. Tomatoes, lycopene, and prostate cancer: Progress and promise. Exp Biol Med (Maywood). 2002;227(10):869-80. https://doi.org/10.1177/153537020222701006 PMid:12424328

Etminan M, Takkouche B, Caamano-Isorna F. The role of tomato products and lycopene in the prevention of prostate cancer: A meta-analysis of observasional studies. Cancer Epidemiol Biomarkers Prev. 2004;13(3):340-5. PMid:15006906

Siler U, Herzog A, Spitzer V, Seifert N, Denelavas A, Hunziker PB, et al. Lycopene effects on rat normal prostate and prostate tumor tissue. J Nutr. 2005;135(8):2050S-2. https://doi.org/10.1093/jn/135.8.2050s PMid:16046739

Tang L, Jin T, Zeng X, Wang JS. Lycopene inhibits the growth of human androgen-independent prostate cancer cells in vitro and in balb/c nude mice. J Nutr. 2005;135(2):287-90. https://doi.org/10.1093/jn/135.2.287 PMid:15671228

Obermuller-Jevic UC, Olano-Martin E, Corbacho AM, Eiserich JP, van der Vliet A, Valacchi G, et al. Lycopene inhibits the growth of normal human prostate epithelial cells in vitro. J Nutr. 2003;133(11):3356-60. https://doi.org/10.1093/jn/133.11.3356 PMid:14608044

Van Breemen RB, Pajkovic N. Multitargeted therapy of cancer by lycopene. Cancer Lett. 2008;269(2):339-51. https://doi.org/10.1016/j.canlet.2008.05.016 PMid:18585855

Khan N, Adhami VM, Mukhtar H. Apoptosis by dietary agents for prevention and treatment of cancer. Biochem Pharmacol. 2008;76(11):1333-9. https://doi.org/10.1016/j.bcp.2008.07.015 PMid:18692026

Freshney RI. Culture of Animal Cell: A Manual of Basic Technique in Primary Culture. 5th ed. New Jersey: John Wiley and Sons; 2005. p. 175-97.

Liu X, Allen JD, Arnold JT, Blackman MR. Lycopene inhibits IGF-I signal transduction and growth in normal prostate epithelial cells by decreasing DHT-modulated IGF-I production in co-cultured reactive stromal cells. Carcinogenesis. 2008;29(4):816-23. https://doi.org/10.1093/carcin/bgn011 PMid:18283040

Hantz HL, Young LF, Martin KR. Physiologically attainable concentrations of lycopene induce mitochondrial apoptosis in lncap human prostate cancer cells. Exp Biol Med (Maywood). 2005;230(3):171-9. https://doi.org/10.1177/153537020523000303 PMid:15734720

Gorrini C, Harris IS, Mak TW. Modulation of oxidative stress as an anticancer strategy. Nat Rev Drug Discov. 2013;12(12):931-47. https://doi.org/10.1038/nrd4002 PMid:24287781

Glasauer A, Chandel NS. Targeting antioxidants for cancer therapy. Biochem Pharmacol. 2014;92(1):90-101. https://doi.org/10.1016/j.bcp.2014.07.017 PMid:25078786

Kanagaraj P, Vijayababu MR, Ravisankar B, Anbalagan J, Aruldhas MM, Arunakaran J. Effect of lycopene on insulin-like growth factor-I, IGF binding protein-3 and IGF Type-I receptor in prostate cancer cells. J Cancer Res Clin Oncol. 2007;133(6):351-9. https://doi.org/10.1007/s00432-006-0177-6 PMid:17219202

Wang XD. Lycopene metabolism and its biological significance. Am J Clin Nutr. 2012;96(5):1214S-22. https://doi.org/10.3945/ajcn.111.032359 PMid:23053559

Sapuntzakis MS, Bowen PE. Role of lycopene and tomato products in prostate health. Biochim Biophy Acta. 2005;1740(2):202-5. https://doi.org/10.1016/j.bbadis.2005.02.004 PMid:15949687

Teodoro AJ, Oliveira FL, Martins NB, Maia GA, Martucci RB, Borojevic R. Effect on lycopene on cell viability and cell cycle progression in human cancer cell line. Cancer Cell Int. 2012;12(1):36. https://doi.org/10.1186/1475-2867-12-36 PMid:22866768

Ford NA, Elsen AC, Zuniga K, Lindhield BL, Erdman JW. Lycopene and apo-12’-lycopenal reduce cell proliferation and alter cell cycle progression in human prostate cancer cells. Nutr Cancer. 2011;63(2):256-63. https://doi.org/10.1080/01635581.2011.523494 PMid:21207319

Palozza P, Colangelo M, Simone R, Catalano A, Boninsegna A, Lanza P, et al. Lycopene induces cell growth inhibition by altering mevalonate pathway and ras signalling in cancer cell lines. Carcinogenesis. 2010;31(10):1813-21. https://doi.org/10.1093/ carcin/bgq157 PMid:20699249