The Effect of Brown Rice on Superoxide Dismutase Level and Non-alcoholic Fatty Liver in an Sprague–Dawley Rat Model of High-fat High-fructose Diet-induced Obesity

Inggita Kusumastuty; Dian Handayani; Shafira Hanifa; Melinda Lisan; Etik Sulistyowati

doi:10.3889/oamjms.2020.5124

Authors

Inggita Kusumastuty Department of Nutrition, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
Dian Handayani Department of Nutrition, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
Shafira Hanifa Department of Nutrition, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
Melinda Lisan Department of Nutrition, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
Etik Sulistyowati Doctoral Programme, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia; Department of Nutrition, Polytechnic of Health, Malang, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2020.5124

Keywords:

Brown Rice, Superoxide dismutase, Nonalcoholic fatty liver disease, High-fat high-fructose diet

Abstract

BACKGROUND: Obesity can cause oxidative stress due to the release of free radical components or reactive oxygen species. The accumulation of excess fat in obesity also causes the occurrence of non-alcoholic fatty liver. Brown rice is a functional food with higher fiber, vitamin, mineral, and antioxidant contents than those in white rice.

AIM: This study was aimed to evaluate the effect of brown rice on the superoxide dismutase (SOD) level and non-alcoholic fatty liver in obese Sprague–Dawley rats.

METHODS: An experimental study using a post-test-only control group design was carried out on a Sprague–Dawley rat model of obesity-induced by a high-fat high fructose (HFHF) diet. Sample selection was done through simple random sampling; rats were divided into five groups, namely the normal diet group (K−), the HFHF diet group (K+), and HFHF diet groups with the addition of brown rice dose I (P1), dose II (P2), and dose III (P3). The measured variable was the SOD level measured using the spectrophotometric method and the calculation of the percentage of fatty liver cells on the results of a hematoxylin-eosin liver scan of a rat’s right lobe.

RESULTS: The dose of brown rice was not affected body weight of the rats significantly compared to the control groups. Then, there were significant differences in the SOD level and fatty liver in at least two groups (p = 0.01 and p = 0.001).

CONCLUSION: This study concludes that the administration of brown rice contributes to the increasing SOD level and decreasing fatty liver in obese model rats.

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