Histopathological Changes of the Flaxseed Extract on Skin Wound Healing in Diabetic Rabbits
DOI:
https://doi.org/10.3889/oamjms.2020.5145Keywords:
Flaxseed, Wound healing, Diabetes, Rabbit, HistopathologyAbstract
BACKGROUND: Wound healing includes phases such as cell migration, extracellular matrix deposition, remodeling, and angiogenesis. There are growing medicines that accelerate wound healing, significantly herbal medications that mainly safe and reliable.
AIM: The aim of the study is to examine the histological changes induced by flaxseed oil during wound healing in diabetic animal model.
METHODS: Forty-five male white New Zealand rabbits divided into two main groups diabetic and non-diabetic each group is divided into three groups (n = 9).Diabetic animal group include: (1) Study group (adding Flaxseed), (2) positive control group (adding fucidin 2% cream), and (3) negative control group (no treatment) same distribution of non-diabetic animals groups. Four linearshape,full-thickness wounds were made in both sides of the backbone skin in each animal. Tissue samples were obtained at days 4th, 7th, and 14th post wounding for microscopical analysis, histopathological parameters included inflammation, re-epithelialization, neovascularization, and surface closure rates.
RESULTS: The study groups (using flax seed) in both diabetic and non-diabetic groups show significant reduction in inflammatory cells infiltration at day 14 (0.67 ± 0.753) (1.67 ± 0.516), and noticeable increase in wound closure rate (4.00+-0.000). Both diabetic and non-diabetic animal groups show high re-epithelialization potential at day 4 and day 7, respectively (2.00 ± 1.549; 4.00 ± 0.00). Diabetic animal study group using flaxseed shows high neovascularization rate at the day 14 (2.17 ± 0.753) compared with positive control diabetic animal group (2.00 ± 0.000).
CONCLUSION: Topical application of flaxseed promoted healing process particularly in diabetic animal model by shortening inflammation phase, elevating surface closure rate, promoting re-epithelialization process, and enhancing neovascularization, flaxseed is suggested as a potential effective herbal base medication for facilitating wound healing.
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Copyright (c) 2020 B. E. Mustafa Al-Ahmad, M. A. Kashmoola, Omar Abdul Jabbar, K. I. Mokhtar, N. Mohamad, R. Abdul Rahim, Mustafa Nazih Shaban (Author)
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