Overview of Helicobacter pylori Detection Using Rapid Urease Test and Giemsa Modification Staining in Chronic Tonsillitis Patients

Ade Asyari; Ferdy Azman; Novialdi Novialdi; Aziz Djamal; Hafni Bachtiar; Yayan Akhyar

doi:10.3889/oamjms.2020.5244

Authors

Ade Asyari Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, Andalas University, Padang, Indonesia
Ferdy Azman Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, Andalas University, Padang, Indonesia
Novialdi Novialdi Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, Andalas University, Padang, Indonesia
Aziz Djamal Department of Microbiology, Faculty of Medicine, Andalas University, Padang, Indonesia
Hafni Bachtiar Department of Public Health, Faculty of Medicine, Andalas University, Padang, Indonesia
Yayan Akhyar Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, Andalas University, Padang, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2020.5244

Keywords:

Chronic tonsillitis, Helicobacter pylori, Rapid urease test, Giemsa modification staining

Abstract

BACKGROUND: Helicobacter pylori (H. pylori) is a Gram-negative bacteria and has been known for its role in causing gastric infection aused diseases such as gastric ulcer. H. pylori also implied to play a role in chronic tonsillitis, but this theory remains controversial. Many researches have different and contradictory results due to difficulty to accurately detect H. pylori in tonsillar tissue. There is still no appropriate method that able to detect H. pylori in tonsil tissue.

AIM: The aim of the study was to detect H. pylori colonization in chronic tonsillitis and understand some of the methods of examination that can be done to detect H. pylori in tonsillar tissue.

METHODS: This study is a descriptive study conducted on 25 respondents. Each sample was taken from patients with chronic tonsillitis who underwent tonsillectomy. Then, the rapid urease test (RUT) and the Giemsa modification staining were carried out to determine the presence of H. pylori.

RESULTS: There were 19 people (76%) positive and 6 people (24%) negative for H. pylori using RUT. On examination with Giemsa modification staining obtained 19 people (76%) positive and 6 people (24%) negative for H. pylori.

CONCLUSION: H. pylori can be found in most of chronic tonsillitis. Combination RUT and Giemsa modification staining examination can be a good option in detecting H. pylori in chronic tonsillitis.

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References

Abo-Amer YE, Sabal A, Ahmed R, Hasan NF, Refaie R, Mostafa SM, et al. Relationship between Helicobacter pylori infection and nonalcoholic fatty liver disease (NAFLD) in a developing country: A cross-sectional study. Diabetes Metab Syndr Obes. 2020;13:619-25. https://doi.org/10.2147/dmso.s237866 PMid:32184641

Siupsinskiene N, Katutiene I, Jonikiene V, Janciauskas D, Vaitkus S. Helicobacter pylori in the tonsillar tissue: A possible association with chronic tonsillitis and laryngopharyngeal reflux. J Laryngol Otol. 2017;131(6):549-56. https://doi.org/10.1017/s0022215117000597 PMid:28318453

Lopes AI, Vale FF, Oleastro M. Helicobacter pylori infectionrecent developments in diagnosis. World J Gastroenterol. 2014;20(28):9299-313. PMid:25071324

Yemisen M, Mete B, Kanbay A, Balkan II, Ozaras R. The role of Helicobacter pylori in upper respiratory system infections: Is it more than colonization? Curr Infect Dis Rep. 2012;14(2):128-36. https://doi.org/10.1007/s11908-012-0237-9 PMid:22311663

Kurtaran H, Uyar ME, Kasapoglu B, Turkay C, Yilmaz T, Akcay A, et al. Role of Helicobacter pylori in pathogenesis of upper respiratory system diseases. J Natl Med Assoc. 2008;100(10):1224-30. https://doi.org/10.1016/s0027-9684(15)31471-1 PMid:18942285

Ozgun E, Tuncel D, Tan A, Albayrak A, Sayhan SE, Bozlak N. Is Helicobacter pylori a possible etiopathogenic factor in chronic tonsillitis? J Clin Anal Med. 2015;6(3):312-5. https://doi.org/10.4328/jcam.2020

Lin HC, Wu PY, Friedman M, Chang HW, Wilson M. Difference of Opinion. Valley View, Ohio: Recycling Today Global; 1999. p. 16.

Wibawa T, Surono A, Widodo I. Isolation of viable Helicobacter pylori in the tonsillar tissues of chronic tonsillitis patients. J Infect Dev Ctries. 2011;5(7):561-4. https://doi.org/10.3855/jidc.1914 PMid:21795828

Hwang MS, Forman SN, Kanter JA, Friedman M. Tonsillar Helicobacter pylori colonization in chronic tonsillitis: Systematic review and meta-analysis. JAMA Otolaryngol Head Neck Surg. 2015;141(3):245-9. https://doi.org/10.1001/jamaoto.2014.3296 PMid:25541915

Kraus J, Nártová E, Pavlík E, Katra R, Šterzl I, Astl J. Prevalence of Helicobacter pylori in adenotonsillar hypertrophy in children. Acta Otolaryngol. 2014;134(1):88-92. https://doi.org/10.3109/00016489.2013.840924 PMid:24256044

Maricić L, Vceva A, Vcev A, Zubcić Z. The role of tonsillectomy in the prevention of Helicobacter pylori infection. Coll Antropol. 2015;39(3):775-8. PMid:26898080

Di Bonaventura G, Neri M, Neri G, Catamo G, Piccolomini R. Do tonsils represent an extragastric reservoir for Helicobacter pylori infection. J Infect. 2001;42(3):221-2. https://doi.org/10.1053/jinf.2001.0815 PMid:11545561

Eyigor M, Eyigor H, Gultekin B, Aydin N. Detection of Helicobacter pylori in adenotonsiller tissue specimens by rapid urease test and polymerase chain reaction. Eur Arch Otorhinolaryngol. 2009;266(10):1611-3. https://doi.org/10.1007/s00405-008-0903-6 PMid:19130070

Payão SL. Helicobacter pylori and its reservoirs: A correlation with the gastric infection. World J Gastrointest Pharmacol Ther. 2016;7(1):126-32. PMid:26855818

Moghaddam YJ, Rafeey M, Radfar R. Comparative assessment of Helicobacter pylori colonization in children tonsillar tissues. Int J Pediatr Otorhinolaryngol. 2009;73(9):1199-201. https://doi.org/10.1016/j.ijporl.2009.05.005

Unver S, Kubilay U, Sezen OS, Coskuner T. Investigation of Helicobacter pylori colonization in adenotonsillectomy specimens by means of the CLO test. Laryngoscope. 2001;111(12):2183-6. https://doi.org/10.1097/00005537-200112000-00021 PMid:11802022

Rotimi O, Cairns A, Gray S, Moayyedi P, Dixon MF. Histological identification of Helicobacter pylori: Comparison of staining methods. J Clin Pathol. 2000;53(10):756-9. https://doi.org/10.1136/jcp.53.10.756 PMid:11064668

Fazaeli A. State of the globe: Diagnostic tests to detect Helicobacter pylori tonsillitis. J Glob Infect Dis. 2012;4(2):99-101. PMid:22754243

Uotani T, Graham DY. Diagnosis of Helicobacter pylori using the rapid urease test. Ann Transl Med. 2015;3(1):9. PMid:25705641

Nártová E, Kraus J, Pavlík E, Lukeš P, Katra R, Plzák J, et al. Presence of different genotypes of Helicobacter pylori in patients with chronic tonsillitis and sleep apnoea syndrome. Eur Arch Otorhinolaryngol. 2014;271(3):607-13. https://doi.org/10.1007/s00405-013-2607-9 PMid:23864246

Lukeš P, Pavlík E, Potuznikova B, Nartova E, Foltynova E, Plzak J, et al. Detection of Helicobacter pylori in oropharyngeal lymphatic tissue with real-time PCR and assessment of its carcinogenic potential. Eur Arch Otorhinolaryngol. 2014;271(2):399-405. https://doi.org/10.1007/s00405-013-2574-1 PMid:23744180

Güçlü O, Akçali A, Şahin EM, Tekin K, Barutçu O, Otkun MT, et al. Relationship between Helicobacter pylori adenotonsillar colonization and frequency of adenotonsillitis in children. Balkan Med J. 2013;30(3):301-4. https://doi.org/10.5152/balkanmedj.2013.8585 PMid:25207124