Topical Instillation of Resveratrol Preconditioned Wharton’s Jelly Mesenchymal Stem Cell Secretome Preserves Ocular Surface in Experimental Model of Severe Dry Eye Disease

Authors

  • Diandra Astaridewi Department of Ophthalmology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia
  • Evelyn Komaratih Department of Ophthalmology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia
  • Yuyun Rindiastuti Department of Ophthalmology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia
  • Yohanes Widodo Wirohadidjojo Department of Dermatovenerology, Faculty of Medicine, Universitas Gadjah Mada, Dr. Sardjito General Academic Hospital, Radioputro Building, Yogyakarta, Indonesia
  • Djoko Legowo Department of Pathology Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Mulyorejo, Surabaya, Indonesia
  • Ni Made Inten Lestari Department of Ophthalmology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia
  • Diandra Astari Dewi Department of Ophthalmology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia
  • I. Made Satya Department of Ophthalmology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia
  • Fedik A. Rantam Stem Cell Research and Development Center, Universitas Airlangga, Tropical Disease Center Building, Kampus C Mulyorejo, Surabaya, Indonesia
  • Cita R. S. Prakoeswa Department of Dermatovenerology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2020.5305

Keywords:

Mesenchymal stem cell, Resveratrol, Secretome, Dry eye disease, Ocular surface

Abstract

BACKGROUND: Secretome derived from Wharton’s jelly mesenchymal stem cells (WJ-MSC) has a beneficial effect for ocular surface regeneration; however, the high amount of vascular endothelial growth factor (VEGF) remains a challenge for its application.

AIM: The aim of the study was to investigate the effect of resveratrol (RV) (VEGF reducing agent) preconditioned WJ-MSC secretome in Concanavalin A-induced severe dry eye model.

METHODS: Pre- and post-experimental study composed of topical instillation of WJ-MSC secretome group, balanced salt solution control group, and normal control group. Tear production, tear break-up time (TBUT), corneal fluorescein dye staining, VEGF level in aqueous tear, goblet cell density, and inflammatory cells in the ocular surface were analyzed.

RESULTS: Topical instillation of RV preconditioned WJ-MSC secretome successfully improved tear film production (p = 0.008) and TBUT (p = 0.008), promoted goblet cell restoration (p = 0.023) and reduced corneal fluorescein staining (p = 0.003), while inhibited infiltration of inflammatory cells (p = 0.01) and secretion of VEGF in aqueous tear (p = 0.003).

CONCLUSION: Topical instillation of RV preconditioned WJ-MSC secretome has great potential as cell-free based therapy to preserve ocular surface in an experimental model of severe Dry eye disease.

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Published

2020-10-19

How to Cite

1.
Astaridewi D, Komaratih E, Rindiastuti Y, Wirohadidjojo YW, Legowo D, Lestari NMI, Dewi DA, Satya IM, Rantam FA, Prakoeswa CRS. Topical Instillation of Resveratrol Preconditioned Wharton’s Jelly Mesenchymal Stem Cell Secretome Preserves Ocular Surface in Experimental Model of Severe Dry Eye Disease. Open Access Maced J Med Sci [Internet]. 2020 Oct. 19 [cited 2024 Mar. 29];8(B):1116-23. Available from: https://oamjms.eu/index.php/mjms/article/view/5305