Evaluation of the Effect of Platelet Rich Plasma on Wound Healing in the Tongue of Normal and Streptozotocin-induced Diabetic Albino Rats: Histological, Immunohistochemical, and Ultrastructural Study

Authors

  • Mary Moheb Ramzy Department of Oral Biology, Faculty of Dentistry, Minia University, Minya, Egypt
  • Tarik Ahmed Essawy Department of Oral Biology, Faculty of Oral and Dental Medicine, Cairo University, Giza, Egypt
  • Ali Shamaa Department of Oral Biology, Faculty of Dentistry, Minia University, Minya, Egypt
  • Saher Sayed Ali Mohamed Department of Oral Biology, Faculty of Dentistry, Minia University, Minya, Egypt

DOI:

https://doi.org/10.3889/oamjms.2020.5366

Keywords:

Platelet rich plasma, Wound healing, Diabetes, Rat, Tongue, p63, Vimentin, TEM

Abstract

Background: Delayed healing of diabetic wounds has been well-documented. Currently, the use of platelet-rich plasma (PRP) has attracted great attention in many medical fields including wound healing.

Aim: Histological, immunohistochemical and ultrastructural evaluation of the effect of PRP on wound healing in the tongue of normal and Streptozotocin-induced diabetic albino rats.

Methodology: A total number of 108 adult male albino rats with average weight 200gm, were used in the study. The animals were classified into two main groups: non-diabetic and diabetic groups. Each group was further divided into three subgroups: non- treated wound, PRP-treatment before wound, and PRP-treatment after wound. Tongue specimens were dissected on postoperative days 1, 3, and 7. The specimens were examined histologically by H&E, immunohistochemically by p63 and vimentin, and ultra-structurally by TEM.

Results:  The most accelerated wound healing was revealed in the subgroups treated with PRP before the wound, whether non-diabetic or diabetic, which occurred very early at the 3rd day postoperative in both cases. While complete wound healing was revealed at the 7th day postoperative in both the non-diabetic and diabetic subgroups treated with PRP after the wound, which was like the non-diabetic control subgroup. Whilst, the diabetic non-treated subgroup only showed partial wound healing at the 7th day postoperative.

Conclusion: A single injection of PRP could be used as a prophylactic to prevent expected impaired wound healing in diabetic oral mucosal wounds and to enhance wound healing in non-diabetic wounds. PRP could be used as a therapeutic to enhance wound healing in diabetic and non-diabetic oral mucosal wounds.

Key Words: platelet rich plasma, wound healing, diabetes, rat, tongue, p63, vimentin, TEM

BACKGROUND: Delayed healing of diabetic wounds has been well-documented. At present, the use of platelet-rich plasma (PRP) has attracted great attention in many medical fields including wound healing.

AIM: Histological, immunohistochemical, and ultrastructural evaluation of the effect of PRP on wound healing in the tongue of normal and streptozotocin-induced diabetic albino rats.

METHODOLOGY: A total number of 108 adult male albino rats with average weight 200 g were used in the study. The animals were classified into two main groups: Non-diabetic and diabetic groups. Each group was further divided into three subgroups: Non-treated wound, PRP-treatment before wound, and PRP-treatment after wound. Tongue specimens were dissected on post-operative days 1, 3, and 7. The specimens were examined histologically by H&E, immunohistochemically by p63 and vimentin, and ultrastructurally by TEM.

RESULTS: The most accelerated wound healing was revealed in the subgroups treated with PRP before the wound, whether non-diabetic or diabetic, which occurred very early at the 3rd day post-operative in both cases. While complete wound healing was revealed at the 7th day post-operative in both the non-diabetic and diabetic subgroups treated with PRP after the wound, which was like the non-diabetic control subgroup. While, the diabetic non-treated subgroup only showed partial wound healing at the 7th day post-operative.

CONCLUSION: A single injection of PRP could be used as a prophylactic to prevent expected impaired wound healing in diabetic oral mucosal wounds and to enhance wound healing in non-diabetic wounds. PRP could be used as a therapeutic to enhance wound healing in diabetic and non-diabetic oral mucosal wounds.

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Published

2020-09-25

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1.
Ramzy MM, Essawy TA, Shamaa A, Mohamed SSA. Evaluation of the Effect of Platelet Rich Plasma on Wound Healing in the Tongue of Normal and Streptozotocin-induced Diabetic Albino Rats: Histological, Immunohistochemical, and Ultrastructural Study. Open Access Maced J Med Sci [Internet]. 2020 Sep. 25 [cited 2024 Mar. 28];8(A):666-89. Available from: https://oamjms.eu/index.php/mjms/article/view/5366