The Association of Antioxidants Gene Polymorphisms (SOD2 Ala16Val, GPx1 Pro198Leu, GSTP1 Ile105Val, and Cat −21 A/T) and Risk of Type 2 Diabetes Mellitus

Mutiara Indah Sari; Siti Syarifah; Milahayati Daulay; Zaimah Z. Tala

doi:10.3889/oamjms.2020.5397

Authors

Mutiara Indah Sari Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Siti Syarifah Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Milahayati Daulay Department of Physiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Zaimah Z. Tala Departement of Clinical Nutrition, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2020.5397

Keywords:

Superoxide dismutase 2 Ala16Val, Glutathione peroxidase Pro198Leu, Glutathione S-transferase Pi1 Ile105Va, Cat −21 A/T; Polymorphisms, Type 2 diabetes mellitus

Abstract

BACKGROUND: Antioxidant gene polymorphism is one of the genetic risk factors associated with type 2 diabetes mellitus (T2DM) incidence.

AIM: This study was to analyze the association of superoxide dismutase 2 (SOD2) Ala16VAl, glutathione peroxidase (GPx1) Pro198Leu, glutathione S-transferase Pi1 (GSTP1) Ile105Val, and Cat −21 A/T gene polymorphisms and risk of T2DM.

METHODS: We genotyped deoxyribonucleic acid of 120 T2DM patients and 80 healthy control by polymerase chain reaction and restriction fragment length polymorphism method, using a specific restriction enzyme.

RESULTS: This study showed that the Val/Val of SOD2 was significantly associated with an increased risk of T2DM compared to the Ala/Ala+Ala/Val (p = 0.011; odds ratio [OR] = 2.220; confidence interval [CI] = 1.234–3.992). The TT genotype of Cat gene was also significantly associated with an increased risk of T2DM compared to the AA genotype (p = 0.027; OR = 5.000; CI = 1.079–23.176) and TT genotype to the AA+AT genotype (p = 0,030; OR = 4.738; CI = 1.039–21.600). However, there was no difference in all genetic models of GPx1 Pro198Leu and GSTP1 Ile105Val gene polymorphisms (p > 0.05).

CONCLUSION: This study indicates that the Val/Val under the recessive model of SOD2 gene also TT genotype under the co-dominant model of Cat gene and TT genotype under the recessive model of Cat gene were associated with risk factors for T2DM occurrence.

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