Interleukin-4 −33 C/T Gene Polymorphisms are not Associated with Type 2 Diabetes Mellitus

Milahayati Daulay; Mutiara Indah Sari

doi:10.3889/oamjms.2020.5419

Authors

Milahayati Daulay Department of Physiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Sumatera Utara, Indonesia
Mutiara Indah Sari Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara, Medan, Sumatera Utara, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2020.5419

Keywords:

Diabetes, Gene, Interleukin, Polymorphisms, Sumatera Utara

Abstract

BACKGROUND: Various studies have shown variations in the interleukin-4 (IL) gene associated with type 2 diabetes mellitus (T2DM) in different populations and ethnicities.

AIM: The objective of this study was to determine the genetic variation of the IL-4 −33 C/T gene in some T2DM patients compared with healthy control in Medan, Sumatera Utara province, Indonesia.

METHODS: DNA from T2DM patients and healthy control was isolated, then the determination of the IL-4 −33 C/T gene polymorphism was carried out using the polymerase chain reaction-restriction fragment length polymorphism technique.

RESULTS: In all samples of this study, IL-4 −33 C/T gene polymorphisms were found in patients with T2DM and healthy control but were not statistically significant.

CONCLUSION: The IL-4 −33 C/T gene polymorphism has no relationship with the incidence of T2DM in a portion of our population in Medan, Sumatera Utara province, Indonesia.

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