Sustained Tau Phosphorylation and Microglial Activation Following Repetitive Traumatic Brain Injury
DOI:
https://doi.org/10.3889/oamjms.2020.5471Keywords:
Activated microglia, Repetitive traumatic brain injury, Phosphorylated tauAbstract
BACKGROUND: Repetitive traumatic brain injury (TBI), even without acute sequela, can induce a delayed neurodegenerative with overexpression of phosphorylated tau (p-tau) as hallmark, caused by chronic inflammation mediated in part by microglial activation.
AIM: The aim of this study was to examine the dynamics of p-tau accumulation and microglial activation following repetitive TBI.
MATERIALS AND METHODS: Thirty Sprague–Dawley rats were randomized into a sham control group and two treatment groups receiving three successive closed-skull impacts (TBI model) from a 40-g mass dropped from a 1-m height on alternating days (days 0, 1, 3, and 7). The first treatment group was sacrificed on the last day of trauma and the second treatment group after 7 days of no trauma. The expression level of p-tau was evaluated by AT-8 antibody immunostaining and microglial activation by anti-CD-68 immunostaining.
RESULTS: Immunoexpression of AT-8 was significantly elevated 7 days after TBI compared to the last day of trauma and compared to the sham control group, while CD-68 expression was significantly higher than sham controls on the last day of trauma and remained elevated for 7 days without trauma.
CONCLUSION: The study showed that brain trauma can induce p-tau overexpression and microglial activation that is sustained during the non-trauma period.
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Copyright (c) 2020 Andre Marolop Pangihutan Siahaan, Rr Suzy Indharty, Jessy Chrestella, Wismaji Sadewo, Steven Tandean, Siti Syarifah (Author)
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