Antimicrobial Activity of Ultrasonic Extracts of Two Chemotypes of Thymus serpyllum L. of Central Kazakhstan and their Polyphenolic Profiles

Svetlana Ivasenko; Perizat Orazbayeva; Krystyna Skalicka–Wozniak; Agnieszka Ludwiczuk; Alexandr Marchenko; Margarita Ishmuratova; Ewa Poleszak; Izabela Korona-Glowniak; Saule Akhmetova; Islambek Karilkhan; Irina Loseva

doi:10.3889/oamjms.2021.5520

Authors

Svetlana Ivasenko Department of Pharmaceutical Disciplines and Chemistry, Karaganda Medical University, Karaganda, Kazakhstan
Perizat Orazbayeva Department of Pharmaceutical Disciplines and Chemistry, Karaganda Medical University, Karaganda, Kazakhstan
Krystyna Skalicka–Wozniak Department of Pharmacognosy, Medicinal Plant Unit, Medical University of Lublin, Lublin, Poland
Agnieszka Ludwiczuk Department of Pharmacognosy, Medicinal Plant Unit, Medical University of Lublin, Lublin, Poland
Alexandr Marchenko Laboratory of Scientific and Research Center, Karaganda Medical University, Karaganda, Kazakhstan
Margarita Ishmuratova Department of Pharmaceutical Disciplines, Academy “Bolashak,” Karaganda, Kazakhstan
Ewa Poleszak Department of Applied Pharmacy, Medical University of Lublin, Lublin, Poland
Izabela Korona-Glowniak Department of Pharmaceutical Microbiology, Laboratory for Microbiological Diagnostics, Medical University of Lublin, Lublin, Poland
Saule Akhmetova Department of Clinical Immunology, Allergology and Microbiology, Karaganda Medical University, Karaganda, Kazakhstan
Islambek Karilkhan Department of Clinical Immunology, Allergology and Microbiology, Karaganda Medical University, Karaganda, Kazakhstan
Irina Loseva Department of Pharmaceutical Disciplines and Chemistry, Karaganda Medical University, Karaganda, Kazakhstan

DOI:

https://doi.org/10.3889/oamjms.2021.5520

Keywords:

Thymus serpyllum L., chemotypes, ultrasonic extracts, antimicrobial activity

Abstract

BACKGROUND: The medicinal plant of Thymus serpyllum L. in nature, depending on the geographical region, climatic conditions, and growing environment, is represented with some chemotypes. Composition and quantitative content of the basic groups of the biologically active substances can be differed, and thus their biological properties are also various.

AIM: The aim of the study was to determine possibility of the using the ultrasonic extracts of two chemotypes of T. serpyllum L. of Central Kazakhstan as an antimicrobial agent against test strains of microorganisms.

MATERIALS AND METHODS: Two samples of T. serpyllum were extracted with 70% ethanol using ultrasound. The polyphenol content of the ultrasound extracts was determined using the LC-ultraviolet-ESI- tandem mass spectrometry technique. A study of an antimicrobial activity of the ultrasonic extracts was performed with eight strains of Gram-positive bacteria, six strains of Gram-negative bacteria, and four cultures of fungi.

RESULTS: The ultrasonic extracts of two chemotypes of T. serpyllum L. are similar in composition of phenolic compounds but differ in a quantitative content of phenolic acids and flavonoids, except for a rosmarinic acid. The ultrasonic extracts have a wide spectrum of antimicrobial activity, exhibit the bactericidal or bacteriostatic activity against all tested bacteria and fungi at a concentration of 0.0625–20 mg/ml, but differ in their strength of action against test strains of microorganisms.

CONCLUSION: The ultrasonic extracts of two chemotypes of T. serpyllum L. of Central Kazakhstan can be considered as a potential drug with a wide spectrum of antimicrobial activity. The results of chromatographic analysis will be used for standardization of a drug.

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References

Republic of Kazakhstan. State Pharmacopoeia of the Republic of Kazakhstan. Vol. 2. Astana: Republic of Kazakhstan; 2009. p. 802.

State Pharmacopoeia of the USSR. Vol. 6. Moscow: 1990. p. 60.

State Pharmacopoeia of Ukraine. State Prize of the Ukrainian Scientific Pharmacopoeial Center of Legal Education. Vol. 3. Kharkiv: State Pharmacopoeia of Ukraine; 2014. p. 487-490.

The British Pharmacopoeia. Commission Secretariat of the Medicines and Healthcare Products Regulatory Agency. London, United Kingdom: The British Pharmacopoeia; 2015.

Jaric S, Mitrovic M, Pavlovic P. Review of ethnobotanical, phytochemical, and pharmacological study of Thymus serpyllum L. J Evid Based Complementary Altern Med. 2015;2015:1-10. PMid:26265920

Jaric S, Mitrovic M, Karadzic B, Kostić O, Djurjević L, Pavlović M, et al. Plant resources used in Serbian medieval medicine. Ethnobot Ethnomed. 2014;61:1359-79.

Quave CL, Pardo-de-Santayana M, Pieroni A. Medical ethnobotany in Europe: from field ethnography to a more culturally sensitive evidence-based cam? Evid Based Complement Alternat Med. 2012;2012:156846. PMid:22899952

Mati E, de Boer H. Ethnobotany and trade of medicinal plants in the Qaysari Market, Kurdish Autonomous Region, Iraq. J Ethnopharmacol. 2011;133:490-510. PMid:20965241

Pieroni A, Rexhepi B, Nedelcheva A, Hajdari A. One century later: The folk botanical knowledge of the last remaining Albanians of the upper Reka Valley, Mount Korab, Western Macedonia. J Ethnobiol Ethnomed. 2013;9:22.

Rexhepi B, Mustafa B, Hajdari A, Rushidi-Rexhepi J, Quave CL, Pieroni A. Traditional medicinal plant knowledge among Albanians, Macedonians and Gorani in the Sharr Mountains (Republic of Macedonia). Genet Resour Crop Evol. 2013;60:2055-80.

Kayani S, Ahmad M, Zafar M, Sultana S, Khan MP, Ashraf MA, et al. Ethnobotanical uses of medicinal plants for respiratory disorders among the inhabitants of Gallies-Abbottabad, Northern Pakistan. J Ethnopharmacol. 2014;156:47-60. PMid:25153021

Mustafa B, Hajdari A, Krasniqi F, Hoxha E, Ademi H, Quave CL, et al. Medical ethnobotany of the Albanian Alps in Kosovo. J Ethnobiol Ethnomed. 2012;8:6. PMid:22284581

Mustafa B, Hajdari A, Pajazita Q, Syla B, Quave CL, Pieroni A. An ethnobotanical survey of the Gollak region, Kosovo. Genet Resour Crop Evol. 2012;59:739-54.

Mustafa B, Hajdari A, Pieroni A, Pulaj B, Koro X, Quave CL. A cross-cultural comparison of folk plant uses among Albanians, Bosniaks, Gorani and Turks living in south Kosovo. J Ethnobiol Ethnomed. 2015;11:39. PMid:25964167

Gairola S, Sharma J, Bedi YS. A cross-cultural analysis of Jammu, Kashmir and Ladakh (India) medicinal plant use. J Ethnopharmacol. 2014;155(2):925-86. PMid:24952280

Kozuharova E, Lebanova H, Getov I, Benbassat N, Napier J. Descriptive study of contemporary status of the traditional knowledge onmedicinal plants in Bulgaria. Afr J Pharm Pharmacol. 2013;7(5):185-98.

Dei Cas L, Pugni F, Fico G. Tradition of use on medicinal species in Valfurva (Sondrio, Italy). J Ethnopharmacol. 2015;163:113-34.

Carrio E, Rigat M, Garnatje T, Mayans M, Parada M, Valles J. Plant ethnoveterinary practices in two pyrenean territories of Catalonia (Iberian Peninsula) and in two areas of the balearic islands and comparison with ethnobotanical uses in humanmedicine. Evid Based Complement Alternat Med. 2012;2012:896295.

Orazbaeva PZ, Ishmuratova MY, Ivasenko SA, Marchenko AB, Losseva IV. Comparative pharmacognostic study of two chemotypes of Thymus serpyllum L. of Central Kazakhstan. Pharm Kazakhstan. 2018;10(207):42-7.

Tejchman W, Korona-Glowniak I, Malm A, Zylewski M, Suder P. Antibacterial properties of 5-substituted derivatives of rhodanine- 3-carboxyalkyl acids. Med Chem Res. 2017;26(6):1316-24. PMid:28515623

Malm A, Glowniak-Lipa A, Korona-Glowniak I, Baj T. Anti- Helicobacter pylori activity in vitro of chamomile flowers, coneflower herbs, peppermint leaves and thyme herbs a preliminary report. Curr Issues Pharm Med Sci. 2015;28(1):30-2.

French GL. Bactericidal agents in the treatment of MRSA infections--the potential role of daptomycin. J Antimicrob Chemother. 2006;58(6):1107-17. PMid:17040922

Aziz S. Studies on the chemical constituents of Thymus serpyllum. Turk J Chem. 2008;32:605-14.

Boros B, Jakabova S, Dornyei A, Horvath G, Pluhar Z, Kilar F, et al. Determination of polyphenolic compounds by liquid chromatography-mass spectrometry in Thymus species. J Chromatog A. 2010;1217(51):7972-80. PMid:20692666

Bayoub K, Baibai T, Mountassif D, Retmane A, Soukri A. Antibacterial activities of the crude ethanol extracts of medicinal plants against Listeria monocytogenes and some other pathogenic strains. Afr J Biotechnol. 2010;9(27):4251-8.

Alzoreky NS, Nakahara K. Antibacterial activity of extracts from some edible plants commonly consumed in Asia. Int J Food Microbiol. 2003;80(3):223-30. PMid:12423924

Honcharenko V, Tkachenko H, Nachychko V, Prokopiv A, Osadowski Z. Antibacterial properties of ethanolic extracts obtained from leaves of some Thymus L. (Lamiaceae) representatives against Acinetobacter baumannii. Agrobiodiversity Improv Nutr Health Life Qual. 2019;3:14-24.

Nostro A, Cellini L, Di Bartolomeo S, Di Campli E, Grande R, Cannatelli MA, et al. Antibacterial effect of plant extracts against Helicobacter pylori. Phytother Res. 2005;19(3):198-202. PMid:15934015

Orazbaeva PZ, Shakarimova KK, Ivasenko SA, Akhmetova SB, Losseva IV. Patent RK No. 34245 for an Invention dated 26.03.2020. A Method of Obtaining an Ultrasonic Extract from Creeping Thyme (Thymus serpyllum L.), which has an Antibacterial Effect against Helicobacter pylori; 2020.

Orazbaeva PZ, Shakarimova KK, Ivasenko SA, Akhmetova SB, Loseva IV. A Positive Decision on the Grant of a Eurasian Patent Dated 06.04.2020 under Application No. 201800259/28. A Method of Obtaining an Ultrasonic Extract from Creeping Thyme (Thymus serpyllum L.), which has an Antibacterial Effect against Helicobacter pylori; 2020.

Sonmezdag AS, Kelebek H, Selli S. Characterization of aroma-active and phenolic profiles of wild thyme (Thymus serpyllum) by GC-MS-Olfactometry and LC-ESI-MS/MS. Food Sci Technol. 2016;53(4):1957-65. PMid:27413222

Pereira OR, Peres AM, Silva AM, Domingues MR, Cardoso SM. Simultaneous characterization and quantification of phenolic compounds in Thymus x citriodorus using a validated HPLC-UV and ESI-MS combined method. Food Res Int. 2013;54:1773-80.

Varga E, Bardocz A, Belak A, Maraz A, Boros B, Felinger A, et al. Antimicrobial activity and chemical composition of thyme essential oils and the polyphenolic content of different Thymus extracts. Farmacia. 2015;63:357-61.

Ҁakmakҁi E, Deveoglu O, Muhammed A, Fouad A, Torgan E, Karadag R. HPLC-DAD analysis of Thymus serpyllum based natural pigments and investigation of their antimicrobial properties. Pigment Resin Technol. 2014;43(1):19-25.

Berdowska I, Zielinski B, Fecka I, Kulbacka J, Saczko J, Gamian A. Cytotoxic impact of phenolics from Lamiaceae species on human breast cancer cells. Food Chem. 2013;141(2):1313-21.