A Histologic and Histomorphometric Analysis of Bone Tissue Regeneration with Perforated Bone Allograft in Rabbit Femur Defect


  • B. E. Tuleubaev Department of Surgical Diseases, Karaganda Medical University, Karaganda, Kazakhstan
  • E. K. Kamyshansky Laboratory and Pathological Unit, Clinic of Karaganda Medical University, Karaganda, Kazakhstan
  • Saginova Dina Saginova Department of Surgical Diseases, Karaganda Medical University, Karaganda, Kazakhstan
  • E. R. Tashmetov Department of Surgical Diseases, Karaganda Medical University, Karaganda, Kazakhstan
  • A. A. Koshanova Department of Surgical Diseases, Karaganda Medical University, Karaganda, Kazakhstan




Bone regeneration, Bone defect, Bone allograft, Marburg bone bank


AIM: The aim of this study is to provide a comparative histopathological evaluation of the regeneration of bone defect filling with perforated antibiotic-impregnated bone allograft.

MATERIALS AND METHODS: Seventy-two healthy rabbits (24 rabbits in each group) were used for this study. Bone defects (3-mm diameter, 10-mm depth) were created in the femur. Human femoral head prepared according to the Marburg bone bank system was used as a bone allograft. The control group did not receive any filling. The experimental groups were as follows: Group 1 – the defects were filled with bone allografts and Group 2 – Perforated gentamycin-impregnated bone allografts. The animals were euthanized after 14, 30, and 60 days. Evaluations consisted of histology at 14-, 30-, and 60-days post-surgery.

RESULTS: A mature bone formation in the group without a bone allograft occurred after 30 days and the group with an allograft after 14 days. In the groups with an allograft, a bone marrow defect was noted as complete closure after 30 days. Histomorphometric analysis showed that in the group with an antibiotic-impregnated bone, allograft leads to increased resorption of the allograft in the intramedullary space compared to group without antibiotic.

CONCLUSION: We believe that a perforated allograft as a result of clinical trials may be obvious and economically affordable in the treatment of bone defects. The use of gentamycin-impregnated bone allografts may be of value in the prevention and treatment of bone infections.


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How to Cite

Tuleubaev BE, Kamyshansky EK, Saginova SD, Tashmetov ER, Koshanova AA. A Histologic and Histomorphometric Analysis of Bone Tissue Regeneration with Perforated Bone Allograft in Rabbit Femur Defect. Open Access Maced J Med Sci [Internet]. 2021 Jan. 5 [cited 2021 Jul. 31];9(A):12-8. Available from: https://oamjms.eu/index.php/mjms/article/view/5588