The Normal Ratio of Th17 and Th1 Post-mesenchymal Stem Cells Coculture with PBMCs of Systemic Lupus Erythematosus Patients

Azizah Retno Kustiyah; Agung Putra; Taufiqurrachman  Nasihun; Rajesh Ramasamy

doi:10.3889/oamjms.2021.5604

Authors

Azizah Retno Kustiyah Student of Postgraduate Biomedical Science, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia; Department of Pediatric, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia
Agung Putra Stem Cell and Cancer Research
Taufiqurrachman Nasihun Department of Postgraduate Biomedical Science, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia
Rajesh Ramasamy Department of Pathology, Faculty of Medicine and Health Sciences, Stem Cell and Immunity Research Group, Immunology Laboratory, Universiti Putra Malaysia, Selangor, Malaysia

DOI:

https://doi.org/10.3889/oamjms.2021.5604

Keywords:

MSCs, Th1, Th17, SLE

Abstract

BACKGROUND: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by altered T-cell population homeostasis, including Th1 and Th17 populations. Mesenchymal stem cell (MSC)-induced i-Treg in SLE milieu can suppress Th17 cell populations; however, Th1 cell population status as a response to the Th17 decrease remains unclear. Therefore, improving SLE flare by suppressing Th17 and constantly controlling Th1 under normal level through MSCs administration is crucial.

AIM: This study aimed to investigate the role of MSCs in suppressing Th17 cell populations and controlling Th1 to a normal level by in vitro coculturing MSCs with PBMC from SLE patients..

METHODS: This study used a post-test control group design. MSCs were obtained from human umbilical cord blood and characterized according to their surface antigen expression and multilineage differentiation capacities. PBMCs isolated from SLE patients were divided into five groups: Sham, control, and three treatment groups. The treatment groups were treated by coculturing MSCs to PBMCs with a ratio of 1:10, 1:25, and 1:40 for 72 h incubation. Th1 and Th17 cells were analyzed by flow cytometry.

RESULTS: This study showed that there was no significance difference of the percentages of Th1 cells on all treatment groups. On the other hand, the percentages of Th17 were significantly decreased on T1 group. Interestingly, there was also significant decrease of Th1-like Th17 cells population on T1 group.

CONCLUSIONS: MSCs may suppress Th17 and control Th1 to a normal level by in vitro coculturing MSCs with PBMC from SLE.

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