Toothpaste Activity Test of Laban Leaf Methanol Extract (Vitex pinnata) Against the Growth of Streptococcus mutans Bacteria

Cut Nuraskin; Reca Reca; Teuku Salfiyadi; Abdurrahman Abdurrahman; Teuku Iskandar Faisal; Cut Soraya

doi:10.3889/oamjms.2021.5702

Authors

Cut Nuraskin Department of Dental Nursing, Polytechnic of Health, Ministry of Health, Aceh, Indonesia
Reca Reca Department of Dental Nursing, Polytechnic of Health, Ministry of Health, Aceh, Indonesia
Teuku Salfiyadi Department of Dental Nursing, Polytechnic of Health, Ministry of Health, Aceh, Indonesia
Abdurrahman Abdurrahman Department of Nursing, Polytechnic of Health, Ministry of Health, Aceh, Indonesia
Teuku Iskandar Faisal Department of Nursing, Polytechnic of Health, Ministry of Health, Aceh, Indonesia
Cut Soraya Department of Dentistry, Universitas Syiah Kuala, Banda Aceh, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.5702

Keywords:

Laban leaf, methanol extract, Toothpaste, S. mutans growth

Abstract

BACKGROUND: One of the natural ingredients that can be used as a substitute for preventing dental caries is the methanol extract of Laban leaves. Laban contains various compounds that are known to have antibacterial properties. The traditional ability of Laban Leaf Methanol Extract has been proven to treat diseases caused by pathogenic microorganisms.

AIM: The aim of the study was to analyzing the activity test of Laban leaf methanol extract toothpaste (Vitex pinnata) against the growth of Streptococcus mutans bacteria.

METHODS: This type of laboratory experimental research (true experimental design) with post-test only control group design. This research was conducted at the Poltekkes Pharmacy Laboratory of the Ministry of Health in Aceh in July-August. The research sample consisted of Laban leaves collected randomly in the Ie Seu Um Geothermal area of Selawah Agam Aceh Besar. Data analysis was carried out descriptively. Antibacterial activity data were statistically processed with one-way ANOVA at a confidence level of α 0.05.

RESULTS: The organoleptic test results observed for 3 weeks showed that there were differences in color and aroma; the higher the concentration of the resulting color, the darker it was, and resulting in a soft, sweet taste in the toothpaste of laban leaf methanol extract. The pH acidity test results showed an effect of storage time (the week I, week II, and week III) on toothpaste’s pH value. The viscosity test results showed that the toothpaste formula combined with the methanol extract of Laban leaves met the SNI 12-524-1995 standards regarding toothpaste. Toothpaste formula F3. 4.5% has the greatest viscosity, ranging from 241.0 (PDAs), and followed by F2. 240.4%, and F1. 237.6%. The higher the active ingredient concentration, the greater the viscosity value. The foam formation test results show that the foam’s stability is greatly influenced by particle size. The more the particle size, the lower the foam stability. ANOVA test results based on the concentration of significance value (0.000) <0.05, there is a difference in the number of bacterial colonies based on the concentration variable. ANOVA test results based on the repetition of significant values (0.423)> 0.05, which means that all repetitions have the same effect on the number of colonies, so there is no difference in the number of colonies based on the repetition variable.

CONCLUSION: Minimum inhibitory concentration of toothpaste laban leaf methanol extract against S. mutans growth was aimed at a concentration of 4.5%, with an average number of bacterial colonies 108.5 × 10⁻⁷CFU/mL).

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