The Potential of Nano Curcumin in Preventing the Formation of Artificial Antisperm Antibody in Wistar Rats through Inflammatory Pathway Regulation

Didit Pramudhito; Suwandi Sugandi; Ida Parwati; Muchtan Sujatno; Soetojo Soetojo

doi:10.3889/oamjms.2021.5749

Authors

Didit Pramudhito Department of Surgery, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia; Department of Surgery, Dr. Mohammad Hoesin General Hospital, Palembang, Indonesia
Suwandi Sugandi Department of Surgery, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia; Department of Surgery, Hasan Sadikin General Hospital, Bandung, Indonesia
Ida Parwati Department of Clinical Pathology, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia; Department of Clinical Pathology, Hasan Sadikin General Hospital, Bandung, Indonesia
Muchtan Sujatno Department of Pharmacology, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia; Department of Pharmacology, Hasan Sadikin General Hospital, Bandung, Indonesia
Soetojo Soetojo Department of Urology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; Department of Urology, Soetomo General Hospital, Surabaya, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.5749

Keywords:

NF-kB, testicular diseases, curcumin, human IL-10, TNF-α

Abstract

BACKGROUND: Immunological mechanisms of infertility are still poorly understood and controversial, both the cause and treatment. Inflammation, immunology, cell proliferation, cell differentiation, and cell survival are influenced by several proteins, including nuclear factor kappa-B (NFĸB), tumor necrosis factor-α (TNF-α), and interleukin-10 (IL-10).

AIM: This study aimed to explore the potential of nano curcumin to prevent anti-sperm antibodies (ASA) formation due to the testes’ inflammatory process in Wistar rats.

METHODS: This research is an experimental study with a pre-post-test approach with control group. The research subjects were rats (Rattus norvegicus) of the Wistar strain. The induced animals were grouped into three groups: Group 1 received nano curcumin 1 × 80 mg/kg BW orally, Group 2 received dexamethasone 1 × 0.3 mg/kg BW, and Group 3 received placebo aquadest 1 × 1 mL orally. TNF-α, NF-kB, and IL10 levels in serum were examined with enzyme-linked immunosorbent assay.

RESULTS: The nano curcumin treatment showed the ability to reduce the pro-inflammatory cytokine protein TNF-α expression (47.3 ± 2.32) more optimally than dexamethasone treatment (54.4 ± 3.22). Nano curcumin has also shown the ability to reduce the pro-inflammatory cytokine transcription factor, NF-kB (32.5 ± 2.76) more optimally than treatment with dexamethasone (44.6 ± 2.43).

CONCLUSION: Nano curcumin can prevent the formation of ASA in testicular trauma through inhibition of the inflammatory response.

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References

Sabanegh E, Agarwal A. Male infertility. In: Wein AJ, Kavoussi LR, Novick AC, Partin AW, Peters CA, editors. Campbell-Walsh Urology. 10th ed. Philadelphia, PA: Saunders-Elsevier; 2012. p. 616-47. https://doi.org/10.1016/b978-1-4160-6911-9.00021-9

Dohle GR, Jungwirth A, Kopa Z, Giwercman A, Diemer T, Hargreave. Male Infertility. The Netherlands: European Association of Urology Guidelines; 2009. p. 6-61. https://doi.org/10.1016/j.eururo.2012.04.048

Cooper TG, Aitken J, Auger J, Baker HW, Barratt CL, Behre HM, et al. WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction. 5th ed. Switzerland: WHO Press; 2010. p. 108-30.

Lombardo F, Gandini L, Dondero F, Lenzi A. Antisperm immunity in natural and assisted reproduction. Hum Rep Update. 2001;7(5):450-6. PMid:11556491

Oeckinghaus A, Ghosh S. The NF-kappaB family of transcription factors and its regulation. Cold Spring Harb Perspect Biol. 2009;1(4):a000034. https://doi.org/10.1101/cshperspect.a000034 PMid:20066092

Ahn KS, Aggarwal BB. Transcription factor NF-kappaB: A sensor for smoke and stress signals. Ann N Y Acad Sci. 2005;1056:218-33. PMid:16387690

Asadullah K, Sterry W, Volk HD. Interleukin-10 therapy--review of a new approach. Pharmacol Rev. 2003;55(2):241-69. https://doi.org/10.1124/pr.55.2.4 PMid:12773629

Lin CL, Lin JK. Curcumin: A potential cancer chemopreventive agent through supressing NF-ĸB signaling. J Cancer Mol. 2008;4(1):11-6.

Tsai YM, Chien CF, Lin LC, Tsai TH. Curcumin and its nano-formulation: The kinetics of tissue distribution and blood-brain barrier penetration. Int J Pharm. 2011;416(1):331-8. https://doi.org/10.1016/j.ijpharm.2011.06.030 PMid:21729743

Bisht S, Feldmann G, Soni S, Ravi R, Karikar C, Maitra A, et al. Polymeric nanoparticle-encapsulated curcumin (“nanocurcumin”): A novel strategy for human cancer therapy. J Nanobiotechnol. 2007;5:3. https://doi.org/10.1186/1477-3155-5-3 PMid:17439648

Huxford T, Ghosh G. A structural guide to proteins of the NF-kappaB signaling module. Cold Spring Harb Perspect Biol. 2009;1(3):a000075. https://doi.org/10.1101/cshperspect.a000075 PMid:20066103

Lawrence T. The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harb Perspect Biol. 2009;1(6):a001651. https://doi.org/10.1101/cshperspect.a001651 PMid:20457564

Wajant H, Pfizenmaier K, Scheurich. Tumor necrosis factor signaling. Cell Death Differ. 2003;10(1):45-65. https://doi.org/10.1038/sj.cdd.4401189 PMid:12655295

Mosser DM, Zhang X. Interleukin-10: New perspectives on an old cytokine. Immunol Rev. 2008;226:205-18. PMid:19161426

Mocellin S, Marincola FM, Young HA. Interleukin-10 and the immune response against cancer: A counterpoint. J Leukoc Biol. 2005;78(5):1043-51. https://doi.org/10.1189/jlb.0705358 PMid:16204623

Turek PJ. Male reproductive physiology. In: Wein AJ, Kavoussi LR, Novick AC, Partin AW, Peters CA, editors. Campbell-Walsh Urology. 10th ed. Philadelphia, PA: Saunders-Elsevier; 2012. p. 591-615. https://doi.org/10.1016/b978-1-4160-6911-9.00020-7

Bisht S, Khan MA, Bekhit M, Bai H, Cornish T, Mizuma M, et al. A polymeric nanoparticle formulation of curcumin (NanoCurc™) ameliorates CCl4-induced hepatic injury and fibrosis through reduction of pro-inflammatory cytokines and stellate cell activation. Lab Invest. 2011;91(9):1383-95. https://doi.org/10.1038/labinvest.2011.86 PMid:21691262

Cheng CY, Mruk DD. The blood-testis barrier and its implications for male contraception. Pharmacol Rev. 2012;64(1):16-64. https://doi.org/10.1124/pr.110.002790 PMid:22039149

Li MW, Mruk DD, Lee WM, Cheng CY. Cytokines and junction restructuring events during spermatogenesis in the

testis: An emerging concept of regulation. Cytokine Growth Factor Rev. 2009;20(4):329-38. https://doi.org/10.1016/j.cytogfr.2009.07.007 PMid:19651533

Wong EW, Mruk DD, Lee WM, Cheng CY. Regulation of blood-testis barrier dynamics by TGF-beta3 is a Cdc42- dependent protein trafficking event. Proc Natl Acad Sci U S A. 2010;107(25):11399-404. https://doi.org/10.1073/pnas.1001077107 PMid:20534521