The Potential of Nano Curcumin in Preventing the Formation of Artificial Antisperm Antibody in Wistar Rats through Inflammatory Pathway Regulation

Authors

  • Didit Pramudhito Department of Surgery, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia; Department of Surgery, Dr. Mohammad Hoesin General Hospital, Palembang, Indonesia
  • Suwandi Sugandi Department of Surgery, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia; Department of Surgery, Hasan Sadikin General Hospital, Bandung, Indonesia
  • Ida Parwati Department of Clinical Pathology, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia; Department of Clinical Pathology, Hasan Sadikin General Hospital, Bandung, Indonesia
  • Muchtan Sujatno Department of Pharmacology, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia; Department of Pharmacology, Hasan Sadikin General Hospital, Bandung, Indonesia
  • Soetojo Soetojo Department of Urology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; Department of Urology, Soetomo General Hospital, Surabaya, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.5749

Keywords:

NF-kB, testicular diseases, curcumin, human IL-10, TNF-α

Abstract

BACKGROUND: Immunological mechanisms of infertility are still poorly understood and controversial, both the cause and treatment. Inflammation, immunology, cell proliferation, cell differentiation, and cell survival are influenced by several proteins, including nuclear factor kappa-B (NFĸB), tumor necrosis factor-α (TNF-α), and interleukin-10 (IL-10).

AIM: This study aimed to explore the potential of nano curcumin to prevent anti-sperm antibodies (ASA) formation due to the testes’ inflammatory process in Wistar rats.

METHODS: This research is an experimental study with a pre-post-test approach with control group. The research subjects were rats (Rattus norvegicus) of the Wistar strain. The induced animals were grouped into three groups: Group 1 received nano curcumin 1 × 80 mg/kg BW orally, Group 2 received dexamethasone 1 × 0.3 mg/kg BW, and Group 3 received placebo aquadest 1 × 1 mL orally. TNF-α, NF-kB, and IL10 levels in serum were examined with enzyme-linked immunosorbent assay.

RESULTS: The nano curcumin treatment showed the ability to reduce the pro-inflammatory cytokine protein TNF-α expression (47.3 ± 2.32) more optimally than dexamethasone treatment (54.4 ± 3.22). Nano curcumin has also shown the ability to reduce the pro-inflammatory cytokine transcription factor, NF-kB (32.5 ± 2.76) more optimally than treatment with dexamethasone (44.6 ± 2.43).

CONCLUSION: Nano curcumin can prevent the formation of ASA in testicular trauma through inhibition of the inflammatory response.

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Published

2021-02-25 — Updated on 2021-03-08

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How to Cite

1.
Pramudhito D, Sugandi S, Parwati I, Sujatno M, Soetojo S. The Potential of Nano Curcumin in Preventing the Formation of Artificial Antisperm Antibody in Wistar Rats through Inflammatory Pathway Regulation. Open Access Maced J Med Sci [Internet]. 2021 Mar. 8 [cited 2021 May 9];9(A):114-8. Available from: https://oamjms.eu/index.php/mjms/article/view/5749