Co-Chemotherapeutic Effect of n-Hexane Fraction of Binahong (Anredera cordifolia [Tenore] Steen.) on WiDr Colon Cancer Cell Line
Keywords:Anredera cordifolia, antioxidant, cytotoxic, molecular docking, WiDr
BACKGROUND: The incidence of colon cancer ranks the third most common cancer case in Indonesia, with 12.8 cases per 100.000 community. Problems that arise from cancer treatment with chemotherapy are severe side effects. Thus, we develop cancer therapy using a natural material, namely, binahong leaves (Anredera cordifolia).
AIM: This study aims to investigate the co-chemotherapy activity of the n-hexane fraction of binahong (NFB) on the WiDr human colon cancer cells line.
METHODS: The sample was prepared by maceration using ethanol 70% and fractionation using n-hexane. Phytochemical screening was conducted with the thin-layer chromatography method. The antioxidant test was carried out using 1,1 diphenyl-2-dipicrilhydrazil assay, while the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay was designed for cytotoxic tests on WiDr colon cancer cells to identify the single effect and its combination with 5-fluorouracil as chemotherapy agents. Further, in silico test, molecular docking was used to determine the 4’, 6, 7-trihydroxyaurone as an active compound on NFB against inhibitors of κB kinases (IKK) and COX-2 proteins.
RESULTS: Phytochemical screening showed that NFB contained a flavonoid compound. NFB had a weak antioxidant activity with the inhibition concentration (IC50) value of 2851 μg/mL, while NFB had a relatively strong cytotoxic activity on WiDr colon cells with the IC50 value of 191 μg/mL, and had the synergist activity in combination with 5-fluorouracil as a chemotherapy agent. The molecular docking showed the best interaction of 4’, 6, 7-trihydroxyaurone against IKK, and COX-2 proteins with docking scores of −9.0 kcal/mol and −8.1 kcal/mol, respectively.
CONCLUSION: NFB had the potential to inhibit cell growth and had a synergistic effect in combination with 5-fluorouracil on WiDr colon cancer cells line.
Globocan. Coloretal Cancer Incidence and Mortality Worldwide in 2012. Available from: http://www.globocan.iarc.fr/old/ FactSheets/cancers/colorectal-new.asp. [Last accessed on 2020 May 05].
Luanpitpong S, Rojanasakul Y. Chemotherapy induced alopecia. In: Topics in Cancer Survivorship. London: IntechOpen Limited; 2012. https://doi.org/10.5772/24861
Hadian BT. Perencanaan Buku Media Informasi Tentang Manfaat Tanaman Binahong untuk Terapi Herbal pada Penyembuh Luka. Tesis. Bandung: Universitas Komputer Indonesia; 2010.
Leliqia NP, Sukandar EY, Fidrianny I. Overview of efficacy, safety and phytochemical study of Anredera cordifolia (Ten.) steenis. Pharmacologyonline. 2017;1:124-31.
Radix RM, Utami D. Uji Sitotoksik dan Anti-proliferasi Ekstrak Eter Daun Binahong (Anredera cordifolia (Tenore) Steenis) Terhadap sel HeLa; 2018. Available from: https://www.researchgate.net/publication/324476245_Uji_Sitotoksik_dan_ Antiproliferasi_Ekstrak_Eter_Daun_Binahong_Andredera_ cordifolia_Tenore_Steen_terhadap_sel_HeLa. [Last accessed on 2020 may 06]. https://doi.org/10.25273/pharmed.v1i1.2271
Andersen M, Markham KR. Flavonoid: Chemistry, Biochemistry and Applications. Boca Raton: CRC Press; 2006.
Fowler LZ, Koffas MA. 2009. Biosynthesis and biotechnological production of flavanones: Current state and perspectives. Appl Microbiol Biotechnol. 2009;83(5):799-808. https://doi.org/10.1007/s00253-009-2039-z PMid:19475406
Mutiah R. Pengembangan Fitofarmaka Antikanker: Panduan Teknik Pengembangan Obat Herbal Indonesia Menjadi Fitofarmaka. Malang: UIN Maliki Press; 2014.
Chen TR, Drabkowski D, Hay RJ, Macy M, Peterson W Jr. WiDr is a derivative of another colon adenocarcinoma cell line, HT-29. Cancer Genet Cytogenet. 1987;27(1):125-34. https://doi.org/10.1016/0165-4608(87)90267-6 PMid:3472642
Merga YJ, O’Hara A, Burkitt MD, Duckworth CA, Probert CS, Campbell BJ, et al. Importance of the alternative NF-κB activation pathway in inflammation-associated gastrointestinal carcinogenesis. Am J Physiol Gastrointest Liver Physiol. 2016;310(11):G1081-90. https://doi.org/10.1152/ajpgi.00026.2016 PMid:27102559
Slattery ML, Fitzpatrick FA. Convergence of hormones, inflammation, and energy-related factors: A novel pathway of cancer etiology. Cancer Prev Res 2009;2(11):922-30. https://doi.org/10.1158/1940-6207.capr-08-0191
Eberhart CE, Coffey RJ, Radhika A, Giardiello FM, Ferrenbach S, DuBois RN. Up-regulation of cyclooxygenase 2 gene expression in human colorectal adenomas and adenocarcinomas. Gastroenterology. 1994;107(4):1183-8. https://doi.org/10.1016/0016-5085(94)90246-1 PMid:7926468
How to Cite
Copyright (c) 2021 Rifki Febriansah, Harena Anggun Lakshita (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All rights reserved.