Preconditioning of Hypoxic Culture Increases The Therapeutic Potential of Adipose Derived Mesenchymal Stem Cells

Authors

  • Tito Sumarwoto Doctoral Program, Faculty of Medicine, Airlangga University, Surabaya, Indonesia; Department of Orthopaedics and Traumatology, Faculty of Medicine, Prof Soeharso Orthopaedic Hospital, Sebelas Maret University, Surakarta, Indonesia
  • Heri Suroto Department of Orthopaedic and Traumatology, Faculty of Medicine, Dr. Soetomo General Hospital, Airlangga University, Surabaya, Indonesia https://orcid.org/0000-0002-9384-897X
  • Ferdiansyah Mahyudin Department of Orthopaedic and Traumatology, Faculty of Medicine, Dr. Soetomo General Hospital, Airlangga University, Surabaya, Indonesia
  • Dwikora Novembri Utomo Department of Orthopaedic and Traumatology, Faculty of Medicine, Dr. Soetomo General Hospital, Airlangga University, Surabaya, Indonesia
  • Romaniyanto Romaniyanto Department of Orthopaedics and Traumatology, Faculty of Medicine, Prof Soeharso Orthopaedic Hospital, Sebelas Maret University, Surakarta, Indonesia
  • Andhi Prijosedjati Department of Orthopaedics and Traumatology, Faculty of Medicine, Prof Soeharso Orthopaedic Hospital, Sebelas Maret University, Surakarta, Indonesia
  • Pamudji Utomo Department of Orthopaedics and Traumatology, Faculty of Medicine, Prof Soeharso Orthopaedic Hospital, Sebelas Maret University, Surakarta, Indonesia
  • Cita Rosita Sigit Prakoeswa Department of Dermatology and Venereology, Faculty of Medicine, Dr. Soetomo General Hospital, Airlangga University, Surabaya, Indonesia https://orcid.org/0000-0003-3232-095X
  • Fedik Abdul Rantam Department of Microbiology, Virology and Immunology Laboratory, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia; Stem Cell Research and Development Center, Airlangga University, Surabaya, Indonesia
  • Damayanti Tinduh Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Dr. Soetomo General Hospital, Airlangga University, Surabaya, Indonesia
  • Hari Basuki Notobroto Faculty of Public Health, Airlangga University, Surabaya, Indonesia
  • Sholahuddin Rhatomy Department of Orthopaedics and Traumatology, Dr. Soeradji Tirtonegoro General Hospital, Klaten, Indonesia; Faculty of Medicine, Public Health, and Nursing, Gadjah Mada University, Yogyakarta, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.5870

Keywords:

Hypoxic, Culture, Adipose Derived Mesenchymal Stem Cells

Abstract

Various in vitro preconditioning strategies have been implemented to increase the regenerative capacity of MSCs. Among them are modulation of culture atmosphere (hypoxia or anoxia), three-dimensional culture (3D), addition of trophic factors (in the form of growth factors, cytokines or hormones), lipopolysaccharides, and pharmacological agents. Preconditioning mesenchymal stem cells by culturing them in a hypoxic environment, which resembles the natural oxygen environment of the tissues (1% –7%) and not with standard culture conditions (21%), increases the survival of these cells via Hypoxia Inducible Factor-1α (HIF-1a) and via Akt-dependent mechanisms. In addition, the hypoxic precondition stimulates the secretion of pro-angiogenic growth factors, increases the expression of chemokines SDF-1 (stromal cell-derived factor-1) and its receptor CXCR4 (chemokine receptor type 4) - CXCR7 (chemokine receptor type 7) and increases engraftment of stem cell. This review aims to provide an overview of the preconditioned hypoxic treatment to increase the therapeutic potential of adipose-derived mesenchymal stem cells.

 

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References

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2021-10-31

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Sumarwoto T, Suroto H, Mahyudin F, Utomo DN, Romaniyanto R, Prijosedjati A, Utomo P, Prakoeswa CRS, Rantam FA, Tinduh D, Notobroto HB, Rhatomy S. Preconditioning of Hypoxic Culture Increases The Therapeutic Potential of Adipose Derived Mesenchymal Stem Cells. Open Access Maced J Med Sci [Internet]. 2021 Oct. 31 [cited 2026 Oct. 19];9(F):505-1. Available from: https://oamjms.eu/index.php/mjms/article/view/5870

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Vol. 9 No. F (2021): F - Review Articles

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Copyright (c) 2021 Tito Sumarwoto, Heri Suroto, Ferdiansyah Mahyudin, Dwikora Novembri Utomo, Romaniyanto Romaniyanto, Andhi Prijosedjati, Pamudji Utomo, Cita Rosita Sigit Prakoeswa, Fedik Abdul Rantam, Damayanti Tinduh, Hari Basuki Notobroto, Sholahuddin Rhatomy (Author)

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