Investigation of Antidiabetogenic Effect of the Iodine-Selenium Concentrate in Animals with Chronic Alloxan Diabetes of Varying Severity

Fatima S.  Abikenova; Gabit Meyramov; Saule Zhautikova; Khamida Abdikadirova; Cymbat Zhienbayeva; Yulia Talaspekova; Irina Baryshnikova; Assima Karipova; Bakhyt Suleimenova

doi:10.3889/oamjms.2021.5873

Authors

Fatima S. Abikenova Department of Pathology, Karaganda Medical University, Karagandy, Kazakhstan https://orcid.org/0000-0001-6871-303X
Gabit Meyramov Department of Zoology https://orcid.org/0000-0001-6871-303X
Saule Zhautikova Department of Pathology https://orcid.org/0000-0003-1173-490X
Khamida Abdikadirova Department of Pathology
Cymbat Zhienbayeva Department of Pathology https://orcid.org/0000-0003-0563-7214
Yulia Talaspekova Department of Pathology https://orcid.org/0000-0001-6871-303X
Irina Baryshnikova Department of Pathology
Assima Karipova Department of Pathology
Bakhyt Suleimenova Department of Pathology

DOI:

https://doi.org/10.3889/oamjms.2021.5873

Keywords:

Alloxan diabetes, Iodine-selenium concentrate, B-cells, Lipids peroxidation, Antioxidant protection

Abstract

BACKGROUND: The diabetogenic effect of alloxan is known is determined by ability to stimulate lipid peroxydation processes in B-cells of the pancreas.

AIM: to investigate of the possible antidiabetic action of long time prolonged of Iodine-Selenium concentrate action in rats with alloxan diabetes.

METHODS: Reproduction of experimental alloxan diabetes was carried out in rats by a single intravenous injection of alloxan 35-43 mg / kg body weight. The “iodine-selenium” concentrate was administered per os through a tube at the rate of 1.25 ml / 100 g of the concentrate. In experimental animals with mild and heavy diabetes mellitus, the level of glucose in the blood was assessed, products of LPO-AOD; the state of the histostructure of the pancreas and of insulin content in B cells were studied using of aldehyde fuchsin and diethylpseudoisocyanine methods.

RESULTS: Long time prolonged administration of the “iodine-selenium” concentrate (60 days) to animals with mild experimental diabetes mellitus is accompanied by a significant decrease in blood glucose levels by 1.89 times compared to the control (p ˂ 0.05) and of LPO within normal values as by increase of the level of glutathione peroxidase (GPO) by 2.23 times compared with the initial (p˂ 0.01), by prevention of the development of histological changes in pancreatic islets and a slight decrease of insulin content in B-cells. Under similar experimental conditions in animals with severe alloxan diabetes, the level of glycemia significantly decreased from 20.23 ± 2.15 mmol / l to 12.39 ± 1.52 mmol / l as of the level of diene conjugates of erythrocytes and plasma, as decrease of ketodienes, MDA of plasma and in erythrocytes and primary lipid peroxidation products, while remaining elevated, despite an increase in GPO by 50.0% compared with control (p˂0.05) in the presence of histological changes in the pancreatic islets as in experimental diabetes.

CONCLUSION: The antidiabetic effect of the “iodine-selenium” concentrate in rats with mild alloxan diabetes on the level of glycemia, LPO - AOD and state of the histostructure of the pancreas and the content of deposited insulin in pancreatic B-cells, is probably due to antioxidant effect of selenium to stimulate activity of glutathione blocking lipid peroxide and hydrogen peroxide in alloxan diabetes mellitus.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Lensen S. The mechanisms of alloxan-and streptozotocin-induced diabetes. Diabetologia. 2008;51(2):216-26. PMid:18087688 DOI: https://doi.org/10.1007/s00125-007-0886-7

Tyrbera B, Andersson A. Comparative study of alloxan toxicity in islet crafts prepared different species. Diabetologia. 1997;1:119.

El-Soud NA, Khalil M. Antioxidative effects of allium cepa essential oilin streptozotocin induced diabetic rats. Maced J Med Sci. 2010;3(4):344-51. https://doi.org/10.3889/mjms.1857-5773.2010.0118 DOI: https://doi.org/10.3889/MJMS.1857-5773.2010.0118

Dawud FA, Eze ED, Ardja AA, Isa AS, Jimoh A, Bashiru M, et al. Ameliorative effects of Vitamin C and zinc in alloxan-induced diabetes and oxidative stress in Wistar rats. Res J Biol Sci. 2012;4(2):123-9.

Zhautikova SB, Seysembekov TZ, Sadybekova ST. Influence of high-fiber diets on the level of carbohydrate and lipid metabolism indicators in blood serum and liver of alloxanediabetic rats. Astana Med J. 2003;1:83-7.

Meyramov GG, Korchin VI, Kocheryzhkina N. Does diabetogenic activity of xanthurenic acid determined by its chelating properties? Transpl Proc. 1998;30(6):2682-4. https://doi.org/10.1016/s0041-1345(98)00788-x PMid:9745547 DOI: https://doi.org/10.1016/S0041-1345(98)00788-X

Meyramov GG, Abikenova FS, Kikimbaeva AA. Studies of the Mechanisms of Diabetogenic Action of Xanturenic Acid. Diabetes Res Clin Prac. 2000;9(50):154-5. https://doi.org/10.1016/s0168-8227(00)81983-x DOI: https://doi.org/10.1016/S0168-8227(00)81983-X

Meyramova AG. Diabetogenic zinc-binding β-cytotoxic compounds. Prob Endocrinol. 2003;49(2):8-16. DOI: https://doi.org/10.14341/probl11516

Meyramov GG, Meyramova AG. Zinc as cause of diabetes induced by chelators and of its prevention. J Am Diabet Assoc. 2002;51(1):591-2.

Obolsky OA, Kravchenko LV, Avrenyeva LI, Tutelyan VA. Effect of the selenium level in the diet on the activity of IDP-glucuronyltransferase and the metabolism of methoxynadeoxynidol in rats. Nutr Issues. 1998;4:18-23.

Rusetskaya NY, Borodulin VB. Biological activity of organoselenium compounds during intoxication with heavy metal salts. Biomed Chem. 2015;61(4):449-61. https://doi.org/10.18097/pbmc20156104449 DOI: https://doi.org/10.18097/PBMC20156104449

Melnichenko OA, Yaroshuk AP, Maksin VN. On the issue of solving the problem of iodine deficiency in the framework of the yodis program. Med Ecol. 2003;4:185-9.

Suchkova LV. Yodis-concentrate in solving problems of iodine deficiency and prevention of health. Med Ecol. 2012;49(65):263-5.

Dubrovski YA, Yankevich ND. Metallomic and metabolomic approaches in the study of diabetes mellitus, the potential role of selenium and iodine. Biomed J Med. 2019;20:151-4.

Baranov VG, Sokoloverova IM, Gasparyan EG. Experimental diabetes mellitus. In: Role in Clinical Diabetology, L: Science. Netherlands: Elsevier; 1983. p. 240.

Gavrilova VB, Mishkorudny MI. Spectrophotometric determination of the content of hydroperoxide lipids in blood plasma. Lab Work. 1983;3:33-5.

Ushkalova VN, Kadochnikova GD. Use of parameters characterizing LPO activity during human adaptation to new climatic conditions. Bull Exp Biol Med. 1987;5:571-3.

Korobeinikova ON. Modification of the determination of lipid peroxidation products in the reaction with thiobarbituric acid. Lab Delo. 1989;7:8-10.

Goncharenko MS, Latinova AM. Method for the assessment of lipid peroxidation. Lab Work. 1985;1:60-1.

Lvovskaya EI. Spectrophotometric determination of LPO end products. Med Chem Issues. 1991;4:92-3.

Korolyuk MA. Method for determining the activity of catalase. Lab Work. 1988;1:16-9.

Klebanov GI, Krainina MV, Chukaeva II. Changes in SOD activity during stimulation of peripheral blood polymorphonuclear leukocytes. Bull Exp Biol Med. 1990;4:334-6. DOI: https://doi.org/10.1007/BF00840032

Kruglikova AA, Shtutman TM. Glutathione peroxidase and glutathione reductase. The activity of the liver was screwed up for the introduction of sodium selenite. Ukrainian Biochem J. 1976;2:223-7.

Muravleva LE. The role of free radical oxidation in the development of apranosis and the effect of biologically active substances on it. Can Biol Sci. 1988;24:11-16.

Kvistberg D, Lester G, Lasarov A. Staining of insulin with aldehyde fuchsin. J Histochem Cytochem. 1966;14(8):609-11. PMid:4165547 DOI: https://doi.org/10.1177/14.8.609

Meyramov GG, Kikimbaeva AA, Meyramova AG. Fluorescent histochemical method staining of insulin in B-cells of isolated pancreatic islets by diethylpseudoisocyanine chloride. Acta Diabetol. 2005;1(42):66.

Meyramova AG, Kohnert KD, Aitkulov AM, Alina AR, Zhuzbaeva GO, Kystaubaeva ZT, et al. High specific fluorescent method for staining of zinc/insulin complex in pancreatic B-cells. Bull Karaganda State Univ. 2013;4:78-84.

Dunn J, McLetchie I, Shechan H. Necrosis of islets of Langerhans produced experimentally. Lancet. 1943;6242:484-7. https://doi.org/10.1016/s0140-6736(00)42072-6 DOI: https://doi.org/10.1016/S0140-6736(00)42072-6

Okamoto K. Biologische untersuchungen der metalle: Histochemischer nachweis einiger metalle in den geweben, besonders in den Nieren und deren Veran derungen. Trans Soc Pathol Jap. 1942;32:99-105.

Agaev BA, Velikhova DM, Gadzhiev AN. Dynamics of the content of glycosylated hemoglobin and malondialdehyde in experimental diabetes mellitus. Bull Exp Biol Med. 1992;9(114):243-4. DOI: https://doi.org/10.1007/BF00809545

Pavlovic D, Bielakovic G, Kocic C, Sokolovic D, Stojanovic I, Peneva V, et al. In vitro and in vivo loabiton of polyamines inhibitory effect on oxidative stress in experimental diabetes mellitus. Diabetologia. 1997;1(40):370.

Terekhina NA, Khorobrikh OY, Khorobrykh TN. The activity of antioxidant enzymes in erythrocytes of rats with alloxan diabetes. Pathol Physiol Exp Ther. 1998;4:25-6.

Kuglirovskaya TM, Parkhomets PK, Donchenko GV. Correction of diabetic neuropathies with aldoreductase inhibitors and picamilon. Quest Med Chem. 1998;6(44):559-64.

Inchina VI, Korobkov DM, Repina EA. Evaluation of the effect of antioxidant drugs on the processes of lipid peroxidation and the activity of plasma antioxidant defense enzymes in the experiment with alloxin-induced diabetes in combination with hypercholesterolemia. Int Res J. 2017;5(59):134-5.

Okamoto H. Molecular basis of experimental diabetes degeneration oncogenesis and regeneration of pancreatic B-cells of islets of Langerhans. Biolssays. 1985;2:115-20. https://doi.org/10.1002/bies.950020106 DOI: https://doi.org/10.1002/bies.950020106

Korchin VI. Morphofunctional Characteristics of Pancreatic B-cells when Exposed to Damaging and Corrective Factors. Kolkata: Doctor of Medical Sciences; 1994. p. 34. Available from: http://medical-diss.com/medicina/morfofunktsionalnaya-harakteristika-pankreaticheskih-beta-kletok-pri-vozdeystvii-na-nih-povrezhdayuschih-i-korrigiruyusch

Erzhanova SA. Clinical and biochemical evaluation of antioxidant therapy in patients with Type 1 diabetes mellitus. Dis Cand Med Sci 1994;20:492467.

Laurenti O, Bravi MC, Cassone Faldetta M. Glutathione effects on insulin resistance in non-insulin-dependent diabetes mellitus. Diabetologia. 1997;1(40):305.

Ivanov VV, Shakhristova EV, Stepovaya EA, Zhavoronok TV, Novitsky VV. Lipid peroxidation and the glutathione system in adipose tissue of rats with alloxan diabetes. Siberian Sci Med J. 2010;6:101-4.