Semen Analysis and Insight into Male Infertility

Batool Mutar Mahdi

doi:10.3889/oamjms.2021.5911

Authors

Batool Mutar Mahdi Department of Microbiology, Consultant Clinical Immunology, Al-Kindy College of Medicine, University of Baghdad, Baghdad, Iraq

DOI:

https://doi.org/10.3889/oamjms.2021.5911

Keywords:

Infertility, Male, Semen

Abstract

Objectives: Semen analysis is the cornerstone for the valuation of the male partner in the infertile couples. This test has been standardized throughout the world through the World Health Organization (WHO) since the1970s by producing, editing, updating, and disseminating a semen analysis manual and guidelines. A retrospective study to give an insight about male infertility.

Methods: This retrospective study assessed the semen findings of 1000 men evaluated at the Department of Urology, Al-Kindy Teaching Hospital in Baghdad-Iraq between January 2016 and May 2019. Semen analysis were done for them.

Results: According to WHO standard for semen normality, 1000 samples that were analyzed, normospermia was shown in 835 (83.5%)males (95% CI=0.811-0.857) and 12% had oligospermia and the rest 4.5% was azospermia. The normospermic samples had significantly higher levels regarding the following parameters: count per ml (51.30±1.24) (P= 0.001), volume(3.34±2.31)(P=0.0001), pus cell (8.04±1.02)(P=0.0001), motility (22.81±5.8)(P=0.0001), abnormal motility (22.81±5.8)(P=0.0001) and normal (V)(P=0.0001)or abnormal morphology (25.86 ±12.4)(P=0.0002) when compared with oligospermia.

Conclusions: Semen analysis is the keystone of infertile couple. Semen parameters like sperm concentration, motility and morphology, are indicators for male reproductive function. Sperm concentration is declining and there is a significant association between sperm concentration and sperm parameters.

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References

Patel AS, Leong JY, Ramasamy R. Prediction of male infertility by the World Health Organization laboratory manual for assessment of semen analysis: A systematic review. Arab J Urol. 2017;16(1):96-102. https://doi.org/10.1016/j.aju.2017.10.005 PMid:29713540 DOI: https://doi.org/10.1016/j.aju.2017.10.005

Morin SJ, Scott RT. Knowledge gaps in male infertility: A reproductive endocrinology and infertility perspective. Transl Androl Urol. 2018;7(Suppl 3):S283-91. https://doi.org/10.21037/tau.2018.05.02 PMid:30159234 DOI: https://doi.org/10.21037/tau.2018.05.02

Harmoosh SK, Almadfaiee ZA. Estimation of the seminal fluid of subfertile patients of different age groups. J Facult Med Baghdad. 2011;53:428-31.

Seshadri S, Bates M, Vince G, Jones DI. The role of cytokine expression in different subgroups of subfertile men. Am J Reprod Immunol. 2009;62(5):275-82. https://doi.org/10.1111/j.1600-0897.2009.00736.x DOI: https://doi.org/10.1111/j.1600-0897.2009.00736.x

Lazem A, Al-Kaseer E, Al-Diwan J, Al-Hadithi T. Effect of infection on semen parameters in a sample of Iraqi infertile males. J Fac Med Bagdad. 2010;52:274-6.

Arab A, Hadi A, Moosavian SP, Askari G, Nasirian M. The association between serum Vitamin D, fertility and semen quality: A systematic review and meta-analysis. Int J Surg. 2019;71:101-9. https://doi.org/10.1016/j.ijsu.2019.09.025 PMid:31561004 DOI: https://doi.org/10.1016/j.ijsu.2019.09.025

Tocci A, Lucchini C. WHO reference values for human semen. Hum Reprod Update. 2010;16(5):559. https://doi.org/10.1093/humupd/dmq019 PMid:20542895 DOI: https://doi.org/10.1093/humupd/dmq019

Ahadi M, Aliakbari F, Latifi S, Hosseini SJ, Gharib A, Movafagh A, et al. Evaluation of the standardization in semen analysis performance according to the WHO protocols among laboratories in Tehran, Iran. Iran J Pathol. 2019;14(2):142-7. https://doi.org/10.30699/ijp.14.2.142 PMid:31528171 DOI: https://doi.org/10.30699/ijp.14.2.142

Cooper TG, Noonan E, von Eckardstein S, Auger J, Baker HW, Behre HM, et al. World Health Organization reference values for human semen characteristics. Hum Reprod Update. 2010;16(3):231-45. https://doi.org/10.1093/humupd/dmp048 PMid:19934213 DOI: https://doi.org/10.1093/humupd/dmp048

Agha RA, Borrelli MR, Vella-Baldacchino M, Thavayogan R, Orgill DP; STROCSS Group. The STROCSS statement: Strengthening the reporting of cohort studies in surgery. Int J Surg. 2017;46:198-202. https://doi.org/10.1016/j.isjp.2017.08.001 PMid:28890409 DOI: https://doi.org/10.1016/j.isjp.2017.08.001

Ajeel NA, Abdul-Kader RA. Female infertility: A study of risk factors. J Facult Med Baghdad. 2014;56:200-4.

Brugh VM, Lipshultz LI. Male factor infertility. Med Clin North Am. 2004;88(2):367-85. PMid:15049583 DOI: https://doi.org/10.1016/S0025-7125(03)00150-0

Butt F, Akram N. Semen analysis parameters: Experiences and insight into male infertility at a tertiary care hospital in Punjab. J Pak Med Assoc. 2013;63(5):558-62. PMid:23757979

Mortimer D, Templeton AA, Lenton EA, Coleman RA. Semen analysis parameters and their interrelationships in suspected infertile men. Arch Androl. 1982;8(3):165-71. https://doi.org/10.3109/01485018208987035 PMid:7103595 DOI: https://doi.org/10.3109/01485018208987035

Shoaib KM, Irshad A, Musa KB, Faheem T. Evaluation of the gonadotropic ratios among men with varying sperm quality. Pak J Med Res 2005;44:19-22.

Yu S, Rubin M, Geevarughese S, Pino JS, Rodriguez HF, Asghar W. Emerging technologies for home-based semen analysis. Andrology. 2018;6(1):10-9. https://doi.org/10.1111/andr.12441 PMid:29194998 DOI: https://doi.org/10.1111/andr.12441

AL-Marayaty SS, Saeed GT, AL-Ahmed HI. Effect of swim up techniques on sperm motility and DNA integrity versus unprepared semen. J Fac Med Baghdad 2017;59(2):151-5. https://doi.org/10.32007/med.1936/jfacmedbagdad.v59i2.10 DOI: https://doi.org/10.32007/med.1936/jfacmedbagdad.v59i2.10

Virtanen HE, Jørgensen N, Toppari J. Semen quality in the 21st century. Nat Rev Urol. 2017;14(2):120-30. https://doi.org/10.1038/nrurol.2016.261 PMid:28050014 DOI: https://doi.org/10.1038/nrurol.2016.261

Elbardisi H, Majzoub A, Al Said S, Al Rumaihi K, El Ansari W, Alattar A, et al. Geographical differences in semen characteristics of 13 892 infertile men. Arab J Urol 2018;16(1):3-9. https://doi.org/10.1016/j.aju.2017.11.018 PMid:29713531 DOI: https://doi.org/10.1016/j.aju.2017.11.018

Harry F. Declining worldwide sperm counts: Disproving a myth. Urol Clin N Am. 2008;35:137-46. PMid:18423235 DOI: https://doi.org/10.1016/j.ucl.2008.01.001

Khan DA, Khan FA, Sattar A, Naveed AK, Malik IA. Azoospermia in clinical practice at Rawalpindi. Pak Armed Forces Med J. 1992;42:93-5.

Subhan F, Tahir F, Ahmed R, Khan ZU. Oligospermia and its relation with hormonal profile. J Pak Med Assoc. 1995;45(9):246-7. PMid:8683833

Imam ME, Siuf A, Mansour MM, Khalid KE, Yosif N, Elhasan EM, et al. Semen analysis of infertile Sudanese males in Gezira State, Central Sudan. Sudan J Public Health 2009;4:340-4.

Nwafia WC, Igweh JC, Udebuani IN. Semen analysis of infertile Igbo males in Enugu, Eastern Nigeria. Niger J Physiol Sci 2006;21(1-2):67-70. https://doi.org/10.4314/njps.v21i1-2.54254 PMid:17242721 DOI: https://doi.org/10.4314/njps.v21i1-2.54254

Akhtar MS, Akhtar FK. Causes of male infertility. Pak J Med Res. 1991;30:159-62.

Sharma R, Agarwal A, Esteves SC. Cigarette smoking and semen quality: A new meta-analysis examining the effect of the 2010 World Health Organization laboratory methods for the examination of human semen. Eur Urol. 2016;70(4):635-45. https://doi.org/10.1016/j.eururo.2016.05.051 PMid:27113031 DOI: https://doi.org/10.1016/j.eururo.2016.05.051

Subhan F, Tahir, F, Alam W, Sultan S, Shahab M. Seminal and hormonal profiles of fertile and subfertile Pakistani men a study of infertility cases. Pak J Med Res. 2000;39:42-5.

Curi SM, Ariagno JI, Chenlo PH, Mendeluk GR, Pugliese MN, Sardisegovia LM, et al. Asthenozoospermia: Analysis of a large population. Arch Androl 2003;49:343-9. https://doi.org/10.1080/713828220 PMid:12893510 DOI: https://doi.org/10.1080/713828220

Dua AA, Vaidya SR. Sperm motility and morphology as changing parameters linked to sperm count variations. J Postgrad Med. 1996;42(4):93-6. PMid:9715307

Yan X, Dong L, Liu Y, Yang F, Tan K, Li J, et al. Effects of physical exercises on semen quality and reproductive outcomes in male infertility: A protocol for systematic review and meta-analysis of randomized controlled trials. Medicine (Baltimore). 2019;98(41):e17494. https://doi.org/10.1097/md.0000000000017494 PMid:31593115 DOI: https://doi.org/10.1097/MD.0000000000017494