Histology of hematoxylin–eosin and immunohistochemical diabetes rat pancreas after giving combination of moringa leaves (Moringa oleifera) and clove flower (Syzygium aromaticum) extracts

Purnama Ningsih; Sitti Rahmawati; Baharuddin Hamzah; Tri Santoso; Nurbaya Nurbaya; Muhammad Fakhrul Hardani; Ririen Hardani

doi:10.3889/oamjms.2021.5928

Authors

Purnama Ningsih Chemistry Study Program, Mathematics and Sciences Division, Faculty of Teacher Training and Education, Tadulako University, Palu, Indonesia
Sitti Rahmawati Chemistry Study Program, Mathematics and Sciences Division, Faculty of Teacher Training and Education, Tadulako University, Palu, Indonesia https://orcid.org/0000-0002-7954-4822
Baharuddin Hamzah Chemistry Study Program, Mathematics and Sciences Division, Faculty of Teacher Training and Education, Tadulako University, Palu, Indonesia
Tri Santoso Chemistry Study Program, Mathematics and Sciences Division, Faculty of Teacher Training and Education, Tadulako University, Palu, Indonesia
Nurbaya Nurbaya Chemistry Study Program, Mathematics and Sciences Division, Faculty of Teacher Training and Education, Tadulako University, Palu, Indonesia
Muhammad Fakhrul Hardani Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Palu, Indonesia
Ririen Hardani Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Palu, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.5928

Keywords:

Moringa, Cloves, Diabetes, Pancreas, Histology

Abstract

The purpose of this study was to determine the regeneration activity of pancreatic beta cells by a combination of the ethanol extracts of Moringa leaves and Clove flower. These extracts prepared by maceration with ethanol as solvent . This is an experimental laboratory research by using post test only group design . The subject of this research is 40 white male rats of Wistar strain ( Rattus norvegicus ) and were conditioned DM by streptozotozine-nicotinamide induction . The rats randomly divided into 8 groups , and each group consisted of 5 rats . Combination dose of Moringa leaves and Clove flowers extracts used are 150 : 40 mg / kg body weight of mice , 100 : 80 mg / kg body weight of mice and 50 : 120 mg / kg body weight of mice . The rats were sacrificed and the pancreas taken to be made preparations histopathology , staining and observation tissues with HE and IHC methods. The results showed that the staining and observation tissues of heart with HE method acquired the positive control group , the single test group and the combination group were showing the islet on the Langerhans island of the pancreas is bigger than in the negative control group. The morphology of the island of Langerhans increasingly large indicates increasing the regeneration of the cells of the islet beta pancreas; whereas the method of IHC acquired the negative control group seen lack of display intensity of positive reaction Ag & Ab beta cells with are marked with brown cells . In the positive control group , the single test group and the combination group , seen a positive reaction of Ag & Ab insulin-producing beta cells characterized by brown cells and insulin expression dominating pancreatic island of Langerhans.

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PMid:20662697