Photoprotective and Inhibitory Activity of Tyrosinase in Extract and Fractions of Terminalia catappa L.

Authors

  • Maulita Indrisari Doctoral Program, Faculty of Medical, Hasanuddin University, Makassar, South Sulawesi, Indonesia; Faculty of Pharmacy, Hasanuddin University, Makassar, South Sulawesi, Indonesia https://orcid.org/0000-0003-2559-838X
  • Sartini Sartini Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia
  • Upik A. Miskad Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia
  • Khairuddin Djawad Sekolah Tinggi Ilmu Farmasi Makassar, Makassar, South Sulawesi, Indonesia
  • Karlina Amir Tahir Doctoral Program, Faculty of Medical, Hasanuddin University, Makassar, South Sulawesi, Indonesia
  • Nurkhairi Nurkhairi Faculty of Pharmacy, Hasanuddin University, Makassar, South Sulawesi, Indonesia
  • Lukman Muslimin Faculty of Pharmacy, Hasanuddin University, Makassar, South Sulawesi, Indonesia https://orcid.org/0000-0002-5703-3714

DOI:

https://doi.org/10.3889/oamjms.2021.5940

Keywords:

Erythema transmission, Pigmentation transmission, Sun protection factor, Terminalia catappa Linn., Tyrosinase

Abstract

BACKGROUND: Chronic exposure to ultraviolet (UV) radiation causes various skin damages. One of the most significant risks to skin occurrence is cancer and photoaging. Recent strategies for photoprotection have included incorporating natural sunscreens and antityrosinase.

AIM: This study aimed to determine the sun protection factor (SPF) and inhibitory activity of tyrosinase of the extract and fractions of leaves of Terminalia catappa Linn.

METHOD: The dried leaves were macerated with 96% ethanol and fractionated using n-hexane, ethyl acetate, and water. The extract and fractions were screened for their phytochemical profile, and tyrosinase inhibitory activity was evaluated and expressed as IC50. The photoprotective activity of extract and fractions were measured by a UV spectrophotometric.

RESULTS: Among the tested samples, the ethyl acetate fraction showed ultraprotection on erythema transmission rate (%TE), sunblock on pigmentation transmission (%TP), and minimum protection on SPF. Ethyl acetate fraction showed the highest activity to inhibit tyrosinase (IC50 was 50.54±2.37 μg/mL). The phytochemical analysis of ethyl acetate fraction revealed the presence of phenolic and flavonoid compounds.

CONCLUSION: This study’s findings revealed a higher tyrosinase inhibitor and sun protection capacity of ethyl acetate fraction of leaves of T. catappa and suitable to develop as a cosmetic agent.

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Published

2021-05-15

How to Cite

1.
Indrisari M, Sartini S, Miskad UA, Djawad K, Amir Tahir K, Nurkhairi N, Muslimin L. Photoprotective and Inhibitory Activity of Tyrosinase in Extract and Fractions of Terminalia catappa L. Open Access Maced J Med Sci [Internet]. 2021 May 15 [cited 2024 Mar. 29];9(A):263-70. Available from: https://oamjms.eu/index.php/mjms/article/view/5940

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