Evaluation of Antioxidant Activity of Botto-Botto Leaf Fraction (Chromolaena Odorata L.) Using DPPH and ABTS Methods

Karlina Amir  Tahir; Upik A.  Miskad; Khairuddin  Djawad; Sartini Djide; Khaerani Khaerani; Maulita  Indrisari

doi:10.3889/oamjms.2021.5982

Authors

Karlina Amir Tahir Department of Pharmacy, Faculty of Medicine and Health Sciences, UIN Alauddin Makassar, Indonesia; Postgraduate Doctoral Program, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
Upik A. Miskad Department of Anatomical Pathology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
Khairuddin Djawad Department of Dermatology and Venereology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
Sartini Djide Faculty of Pharmacy, Universitas Hasanuddin, Makassar, Indonesia https://orcid.org/0000-0001-8155-4467
Khaerani Khaerani Department of Pharmacy, Faculty of Medicine and Health Sciences, UIN Alauddin Makassar, Indonesia https://orcid.org/0000-0001-8648-9676
Maulita Indrisari Postgraduate Doctoral Program, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.5982

Keywords:

Antioxidant, Botto-Botto (Chromolaena odorata L.), DPPH, ABTS

Abstract

BACKGROUND: Antioxidants are substances that can inhibit the oxidation process, so that they have a protective effect on cells from the dangers of free radicals. One of the plants that has activity as natural antioxidants is Botto-Botto leaves (Chromolaena odorata L.).

AIM: This study aims to determine the antioxidant activity of the Botto-Botto leaf fraction using the DPPH and ABTS methods.

METHODS: The leaves of Botto-Botto (Chromolaena odorata L.) obtained from Takalar District, South Sulawesi are sorted, dried and pollinated. The powder was immersed in 70% ethanol solvent and sonicated. Furthermore, the extraction was carried out by maceration method with 70% ethanol solvent and fractionated using water, n-hexane, and n-butanol as solvents and dried. The fractionation results were continued for antioxidant testing using the DPPH and ABTS methods.

RESULTS: The n-butanol fraction of Botto-Botto leaves had the highest antioxidant activity, namely the strong active category. In the DPPH and ABTS methods, the n-butanol fraction has IC50 values of 33.535 μg / mL and 60.885 μg / mL, respectively.

CONCLUSION: The DPPH and ABTS methods, the n-butanol fraction of botto-botto leaves have strong antioxidant activity compared to other fractions, namely the water fraction and the n-hexane fraction.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Bernhoft A. A brief review on bioactive compounds in plants. In: Bioactive Compounds in Plants-Benefits and Risks for Man and Animals. Vol. 50. The Norwegian Academy of Science and Letters; 2010. p. 11-7.

Puri B, Hall A. Phytochemical Dictionary: A Handbook of Bioactive Compounds from Plants. Boca Raton: CRC Press; 1998.

Sasidharan S, Chen Y, Saravanan D, Sundram KM, Latha LY. Extraction, isolation and characterization of bioactive compounds from plants’ extracts. Afr J Tradit Complement Altern Med. 2011;8(1):1-10. https://doi.org/10.4314/ajtcam.v8i1.60483 PMid:22238476 DOI: https://doi.org/10.4314/ajtcam.v8i1.60483

Das S, Bora N, Rohman MA, Sharma R, Jha AN, Roy AS. Molecular recognition of bio-active flavonoids quercetin and rutin by bovine hemoglobin: An overview of the binding mechanism, thermodynamics and structural aspects through multi-spectroscopic and molecular dynamics simulation studies. Phys Chem Chem Phys. 2018;20(33):21668-84. https://doi.org/10.1039/c8cp02760a DOI: https://doi.org/10.1039/C8CP02760A

Jan AT, Azam M, Siddiqui K, Ali A, Choi I, Haq QM. Heavy metals and human health: Mechanistic insight into toxicity and counter defense system of antioxidants. Int J Mol Sci. 2015;16(12):29592-630. https://doi.org/10.3390/ijms161226183 PMid:26690422 DOI: https://doi.org/10.3390/ijms161226183

Maesaroh K, Kurnia D, Anshori JA. Comparison of the antioxidant activity test methods of DPPH, FRAP and FIC against ascorbic acid, gallic acid and quercetin. Chim Nat Acta. 2018;6(2):93-100. https://doi.org/10.24198/cna.v6.n2.19049 DOI: https://doi.org/10.24198/cna.v6.n2.19049

Zachariades C, Day M, Muniappan R, Reddy GV. Chromolaena odorata (L.) king and robinson (Asteraceae). In: Biological Control of Tropical Weeds using Arthropods. Cambridge: Cambridge University Press; 2009. p. 130-62. https://doi.org/10.1017/cbo9780511576348.008 DOI: https://doi.org/10.1017/CBO9780511576348.008

Adedapo AA, Oyagbemi AA, Fagbohun OA, Omobowale TO, Yakubu MA. Evaluation of the anticancer properties of the methanol leaf extract of Chromolaena odorata on HT-29 cell line. J Pharmacogn Phytochem. 2016;5(2):52.

Parameswari G, Suriyavathana M. In vitro antioxidant activity of Chromolaena odorata (L.) King and Robinson. Int Res J Pharm. 2012;3(11):187-92.

Pitakpawasutthi Y, Thitikornpong W, Palanuvej C, Ruangrungsi N. Chlorogenic acid content, essential oil compositions, and in vitro antioxidant activities of Chromolaena odorata leaves. J Adv Pharm Technol Res. 2016;7(2):37-42. https://doi.org/10.4103/2231-4040.177200 PMid:27144150 DOI: https://doi.org/10.4103/2231-4040.177200

Usunomena U, Efosa EG. Phytochemical analysis, mineral composition and in vitro antioxidant activities of Chromolaena odorata leaves. ARC J Pharm Sci. 2016;2(2):16-20. https://doi.org/10.20431/2455-1538.0202003 DOI: https://doi.org/10.20431/2455-1538.0202003

Mhamdi A, Breusegem FV. Reactive oxygen species in plant development. Development. 2018;145(15):dev164376. https://doi.org/10.1242/dev.164376 PMid:30093413 DOI: https://doi.org/10.1242/dev.164376

Kinnula VL, Crapo JD. Superoxide dismutases in malignant cells and human tumors. Free Radic Biol Med. 2004;36(6):718 44. https://doi.org/10.1016/j.freeradbiomed.2003.12.010 PMid:14990352 DOI: https://doi.org/10.1016/j.freeradbiomed.2003.12.010

Sies H, Jones DP. Reactive oxygen species (ROS) as pleiotropic physiological signalling agents. Nat Rev Mol Cell Biol. 2020;21(7):363-83. https://doi.org/10.1038/s41580-020-0230-3 PMid:32231263 DOI: https://doi.org/10.1038/s41580-020-0230-3

Slater TF. Free radical mechanisms in tissue injury. In: Cell Function and Disease. Berlin: Springer; 1988. p. 209-18. DOI: https://doi.org/10.1007/978-1-4613-0813-3_18

Sridhar K, Charles AL. In vitro antioxidant activity of Kyoho grape extracts in DPPH and ABTS assays: Estimation methods for EC50 using advanced statistical programs. Food Chem. 2019;275:41-9. https://doi.org/10.1016/j.foodchem.2018.09.040 PMid:30724215 DOI: https://doi.org/10.1016/j.foodchem.2018.09.040

Yeo J, Shahidi F. Revisiting DPPH (2, 2-diphenyl-1- picrylhydrazyl) assay as a useful tool in antioxidant evaluation: A new IC100 concept to address its limitations. J Food Bioact. 2019;7: 36-42. https://doi.org/10.31665/jfb.2019.7196 DOI: https://doi.org/10.31665/JFB.2019.7196

Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol. 2004;26(2):211-9.

Handayany GN, Umar I, Ismail I. Formulation and test of the antioxidant effectiveness of the botto’-botto ‘(Chromolaena odorata L.) leaf ethanol extract cream using the DPPH method. J Kesehatan. 2018;11(2):86– 90. https://doi.org/10.24252/kesehatan.v11i2.5944 DOI: https://doi.org/10.24252/kesehatan.v11i2.5944

Rusdi M, Haeria H, Hamzah N, Rauf A, Amriani F. Antiproliferation potential of botto-botto (Chromolaena odorata L.) leaves methanol extract fraction against HeLa cell line. Ad-Dawaa’ J Pharm Sci. 2020;3(1):83-9.