The Effect of Coffee Arabica Gayo Leaf Extract (Coffea arabica L.) in Increasing Phosphoinositide 3-kinase and Glucose Transporter-4 Expression in the Skeletal Muscle
DOI:
https://doi.org/10.3889/oamjms.2021.5985Keywords:
Diabetes mellitus, Gayo Arabica coffee leaf extract, Glucose transporter-4 expression, Phosphoinositide 3-kinase expressionAbstract
BACKGROUND: Diabetes mellitus (DM) is a chronic and progressive metabolic disease characterized by hyperglycemia due to impaired insulin secretion associated with a lack of phosphoinositide 3-kinase (PI3K) and glucose transporter-4 (GLUT-4) expression in the skeletal muscle membrane.
AIM: The aim of the study is to understand the effect of coffee Arabica Gayo leaf extract (Coffea arabica L.) in increasing PI3K expression and GLUT-4 expression in the skeletal muscle membrane.
METHODS: Thirty-five male Wistar rats with Type 2 DM (T2DM) induced using a combination of a high-fat diet for 5 weeks followed by multiple intraperitoneal injections of low-dose streptozotocin (30 mg/kg). Divided into seven groups as such two groups that did not receive treatment and five groups that received treatment. The dosage administered was 150, 200, and 250 mg/kg/day through the nasogastric tube for 30 days. PI3K and GLUT-4 expression in the skeletal muscle membrane was evaluated by Immunohistochemistry in their gastrocnemius muscles.
RESULTS: The study showed an increased expression of PI3K and GLUT-4 expression. There was a significant difference between coffee Arabica Gayo leaf extract and Metformin in increasing GLUT-4 expression (p = 0.036) and PI3K expression between coffee Arabica Gayo dose 250 mg/kg/day and group without treatment (p = 0.008).
CONCLUSION: Coffee Arabica Gayo leaf extract (C. arabica L) at a dose of 250 mg/kg/day can increase PI3K expression in skeletal muscle and a dose of 200 mg/kg/day and 250 mg/kg/day can increase the expression of GLUT-4 in the skeletal muscle membrane greater than metformin.Downloads
Metrics
Plum Analytics Artifact Widget Block
References
International Diabetes Federation. IDF Diabetes Atlas. 7th ed. Brussels, Belgium: In International Diabetes Federation; 2019. Available form: https://www.idf.org/e-library/epidemiology-research/diabetes-atlas.html. [Last accessed on 2020 Dec 20].
PERKENI Perkumpulan Endokrinologi Indonesia. Konsensus Pengelolaan dan Pencegahan Diabetes Mellitus Tipe 2 di Indonesia. Diunduh dari; 2015. Available form: https://www.pbperkeni.or.id. [Last accessed on 2020 Dec 20].
Cheeseman C, Long W. Structure of and functional insight into the GLUT family of membrane transporters. Cell Health Cytoskeleton. 2015;7:167-83. https://doi.org/10.2147/CHC.S60484. DOI: https://doi.org/10.2147/CHC.S60484
Khorami SA, Movahendi A, Huzwah K, Sokhini AM. P13k/Akt pathway in modulating glucose homeostasis and its alteration in diabetes. Ann Med Biomed Sci. 2015;1(2):46-55.
Hussain SA, Marouf BH. Flavonoids as alternatives in treatment of Type 2 diabetes mellitus. Acad J Med Plants. 2013;1(2):31-6. http://doi.org/10.15413/ajmp.2012.0111
Martina SJ, Govindan PA, Wahyuni AS. The difference in effect of Arabica coffee Gayo beans and leaf (Coffea arabica L. Gayo) extract on decreasing blood sugar levels in healthy mice. Maced J Med Sci. 2019;7(20):3363-65. http://doi.org/10.3889/oamjms.2019.423 DOI: https://doi.org/10.3889/oamjms.2019.423
Federer WT. Experimental design: Theory and application. In: Hanafiah KA. Rancangan Percobaan-teori Dan Aplikasi. Vol. 3. Indonesia: Raja Grafindo Persada; 1995. p. 9-10.
McCarty MF. A chlorogenic acid induce increase in GLP-1 Production may mediate the impact of heavy coffee consumption on diabetes risk. Med Hypothesis 2005;64(4):848-53. PMid:15694706 DOI: https://doi.org/10.1016/j.mehy.2004.03.037
Hu X, Wang S, Xu J, Wang DB, Chen Y, Yang GZ. Triterpenoid saponins from Stauntonia chinensis ameliorate insulin resistance via the AMP-activated protein kinase and IR/IRS-1/PI3K/Akt pathways in insulin-resistant HepG2 cells. Int J Mol Sci. 2014;15(6):10446-58. https://doi.org/10.3390/ijms150610446. PMid:24918297 DOI: https://doi.org/10.3390/ijms150610446
Olusola O, Kamde JP. Saponins as a adipokines modulator: A possible therapeutic intervention for Type 2 diabetes. World J Diabetes. 2017;8(7):337-45. PMid:28751956 DOI: https://doi.org/10.4239/wjd.v8.i7.337
Sieniawska E. Activities of tannins-from in vitro studies to clinical trials. Nat Prod Commun. 2015;10(11):1877-84. PMid:26749816 DOI: https://doi.org/10.1177/1934578X1501001118
Yan YX, Xiao HB, Wang SS, Zhao J, He Y, Wang W, et al. Investigation of the relationship between chronic stress and insulin resistance in a chinese population. J Epidemiol. 2016;26(7):355-60. http://doi.org/10.2188/jea.JE20150183 PMid:26830350 DOI: https://doi.org/10.2188/jea.JE20150183
Ahren B. Avoiding hypoglycemia: A key to success for glucose-lowering therapy in Type 2 diabetes. Vasc Health and Risk Manag. 2013;9:155-63. http://doi.org/10.2147/VHRM.S33934 PMid:23637538 DOI: https://doi.org/10.2147/VHRM.S33934
Tajik N, Tajik M, Mack I, Enck P. The potential effects of chlorogenic acid, the main phenolic components in coffee, on health: A comprehensive review of the literature. Eur J Nutr. 2017;56(7):2215-44. http://doi.org/10.1007/s00394-017-1379-1 PMid:28391515 DOI: https://doi.org/10.1007/s00394-017-1379-1
Marques V, Farah A. Chlorogenic acids and related compounds in medicinal plants and infusions. Food Chem. 2009;113(4):1370-6. https://doi.org/10.1016/j.foodchem.2008.08.08. DOI: https://doi.org/10.1016/j.foodchem.2008.08.086
Downloads
Published
How to Cite
License
Copyright (c) 2021 Sake Juli Martina, Aznan Lelo, Dharma Lindarto, Ratna Akbari Ganie, Muhammad Ichwan, Hanifah Yusuf, Syafruddin Ilyas, Iqbal Pahlevi Nasution (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
http://creativecommons.org/licenses/by-nc/4.0
