The Effect of Coffee Arabica Gayo Leaf Extract (Coffea arabica L.) in Increasing Phosphoinositide 3-kinase and Glucose Transporter-4 Expression in the Skeletal Muscle

Authors

  • Sake Juli Martina Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Universitas Sumatera Utara Hospital, Medan, Indonesia
  • Aznan Lelo Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Dharma Lindarto Department of Internal Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Ratna Akbari Ganie Department of Clinical Pathology, Faculty of Medicine, Universitas Sumatera Utara, Medan, IndonesiaDepartment of Clinical Pathology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Muhammad Ichwan Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Hanifah Yusuf Department of Pharmacology, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia
  • Syafruddin Ilyas Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia
  • Iqbal Pahlevi Nasution Department of Pediatric Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.5985

Keywords:

Diabetes mellitus, Gayo Arabica coffee leaf extract, Glucose transporter-4 expression, Phosphoinositide 3-kinase expression

Abstract

BACKGROUND: Diabetes mellitus (DM) is a chronic and progressive metabolic disease characterized by hyperglycemia due to impaired insulin secretion associated with a lack of phosphoinositide 3-kinase (PI3K) and glucose transporter-4 (GLUT-4) expression in the skeletal muscle membrane.

AIM: The aim of the study is to understand the effect of coffee Arabica Gayo leaf extract (Coffea arabica L.) in increasing PI3K expression and GLUT-4 expression in the skeletal muscle membrane.

METHODS: Thirty-five male Wistar rats with Type 2 DM (T2DM) induced using a combination of a high-fat diet for 5 weeks followed by multiple intraperitoneal injections of low-dose streptozotocin (30 mg/kg). Divided into seven groups as such two groups that did not receive treatment and five groups that received treatment. The dosage administered was 150, 200, and 250 mg/kg/day through the nasogastric tube for 30 days. PI3K and GLUT-4 expression in the skeletal muscle membrane was evaluated by Immunohistochemistry in their gastrocnemius muscles.

RESULTS: The study showed an increased expression of PI3K and GLUT-4 expression. There was a significant difference between coffee Arabica Gayo leaf extract and Metformin in increasing GLUT-4 expression (p = 0.036) and PI3K expression between coffee Arabica Gayo dose 250 mg/kg/day and group without treatment (p = 0.008).

CONCLUSION: Coffee Arabica Gayo leaf extract (C. arabica L) at a dose of 250 mg/kg/day can increase PI3K expression in skeletal muscle and a dose of 200 mg/kg/day and 250 mg/kg/day can increase the expression of GLUT-4 in the skeletal muscle membrane greater than metformin.

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Published

2021-11-03

How to Cite

1.
Martina SJ, Lelo A, Lindarto D, Ganie RA, Ichwan M, Yusuf H, Ilyas S, Nasution IP. The Effect of Coffee Arabica Gayo Leaf Extract (Coffea arabica L.) in Increasing Phosphoinositide 3-kinase and Glucose Transporter-4 Expression in the Skeletal Muscle. Open Access Maced J Med Sci [Internet]. 2021 Nov. 3 [cited 2024 Mar. 28];9(A):906-10. Available from: https://oamjms.eu/index.php/mjms/article/view/5985

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