Composite Bacterial Infection Index and Serum Amyloid A Protein in Pulmonary Tuberculosis Patients and their Household Contacts in Makassar

Irda Handayani; Muhammad Nasrum Massi; Yanti Leman; Rosdiana Natzir; Ilhamjaya Patellongi; Subair Subair; Najdah Hidayah; Ayu Andini Wulandari; Handayani Halik

doi:10.3889/oamjms.2021.6114

Authors

Irda Handayani Department of Clinical Pathology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia; Postgraduate (Doctoral) Program, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
Muhammad Nasrum Massi Department of Clinical Microbiology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia; Medical Research Center, Medical Faculty, Universitas Hasanuddin, Makassar, Indonesia
Yanti Leman Department of Pharmacology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
Rosdiana Natzir Department of Biochemistry, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
Ilhamjaya Patellongi Department of Physiology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia; 8Universitas Mega Rezky, Makassar, Indonesia
Subair Subair Postgraduate (Doctoral) Program, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
Najdah Hidayah Postgraduate (Doctoral) Program, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia https://orcid.org/0000-0002-9811-2080
Ayu Andini Wulandari Department of Clinical Microbiology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
Handayani Halik Postgraduate (Doctoral) Program, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia; Medical Research Center, Medical Faculty, Universitas Hasanuddin, Makassar, Indonesia; Universitas Mega Rezky, Makassar, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.6114

Keywords:

Composite bacterial infection index, Serum amyloid A, Pulmonary tuberculosis, Interferon gamma release assay, Household contacts

Abstract

BACKGROUND: Early diagnosis of tuberculosis (TB) cases in limited resource remains challenging. It is urgent to identify the new diagnostic tools which can control the spread of disease with accurate and rapid test.

AIM: This study aimed to investigate the levels of infection markers: Composite bacterial infection index (CBII) and serum amyloid A (SAA) protein in pulmonary TB (PTB), and their healthy household contacts, as the alternative diagnostic markers for TB.

METHODS: CBII and SAA were measured from 44 new PTB patients, and 31 household contact serum samples. The value of CBII was calculated from neutrophils, lymphocytes, monocytes, erythrocyte sedimentation rate, and high-sensitivity C-reactive protein (hs-CRP) level. hs-CRP and SAA levels were quantified from their serum samples using ELISA. QuantiFERON-TB Gold Plus (interferon gamma release assay [IGRA]) was used to screen latent TB infection among household contacts.

RESULTS: Among 31 household contacts, there were 24 positive IGRA results and the rest (n = 7) had negative results. PTB patients exhibited significantly higher level CBII in the serum specimens, than those in household contact (p < 0.0001). There was no significant difference in the SAA level between TB cases and household contacts (p = 0.679).

CONCLUSIONS: CBII can be used as one of the biomarkers for the identification of PTB from the serum specimens.

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