Antimicrobial Activity and Polyphenol Profiles of Hydroalcoholic Extracts of Thymus rasitatus Klokov and Thymus eremita Klokov

Svetlana  Ivasenko; Ainur Zhumabekova; Agnieszka  Ludwiczuk; Krystyna  Skalicka–Wozniak; Alexandr  Marchenko; Margarita  Ishmuratova; Eva  Poleszak; Izabela  Korona-Glowniak; Saule  Akhmetova; Islambek  Karilkhan; Irina  Loseva

doi:10.3889/oamjms.2021.6117

Authors

Svetlana Ivasenko Department of Pharmaceutical Disciplines and Chemistry, School of Pharmacy, Karaganda Medical University, Karaganda, Kazakhstan
Ainur Zhumabekova Department of Pharmaceutical Disciplines and Chemistry, School of Pharmacy, Karaganda Medical University, Karaganda, Kazakhstan https://orcid.org/0000-0002-6694-8860
Agnieszka Ludwiczuk Independent Laboratory of Natural Products Chemistry, Department of Pharmacognosy, Medical University of Lublin, Lublin, Poland https://orcid.org/0000-0003-1108-613X
Krystyna Skalicka–Wozniak Independent Laboratory of Natural Products Chemistry, Department of Pharmacognosy, Medical University of Lublin, Lublin, Poland https://orcid.org/0000-0002-9313-5929
Alexandr Marchenko The Shared Laboratory of Scientific and Research Center, Karaganda Medical University, Karaganda, Kazakhstan https://orcid.org/0000-0002-0325-7659
Margarita Ishmuratova Department of Pharmaceutical Disciplines, Academy “Bolashak”, Karaganda, Kazakhstan https://orcid.org/0000-0002-1735-8290
Eva Poleszak Department of Applied Pharmacy, Medical University of Lublin, Lublin, Poland
Izabela Korona-Glowniak Department of Pharmaceutical Microbiology with Laboratory for Microbiological Diagnostics, Medical University of Lublin, Lublin, Poland https://orcid.org/0000-0003-1634-0387
Saule Akhmetova Department of Biomedicine, Karaganda Medical University, Karaganda, Kazakhstan
Islambek Karilkhan Department of Biomedicine, Karaganda Medical University, Karaganda, Kazakhstan
Irina Loseva Department of Pharmaceutical Disciplines and Chemistry, School of Pharmacy, Karaganda Medical University, Karaganda, Kazakhstan

DOI:

https://doi.org/10.3889/oamjms.2021.6117

Keywords:

Thymus rasitatus Klokov, Thymus eremita Klokov, Ultrasound assisted extraction, Antimicrobial activity

Abstract

BACKGROUND: A possible reduction in stocks of medicinal plant raw materials of Thymus serpyllum L. and Thymus vulgaris L. leads to the need to expand the raw material base of the official medicinal plants with using of endemic species of the flora of Kazakhstan, in particular, Thymus rasitatus Klokov, and Thymus eremita Klokov.

AIM: The aim of the study was to study the possibility of using 70% ethanol extracts of T. rasitatus and T. eremita as antimicrobial agents.

MATERIALS AND METHODS: The aerial parts of T. rasitatus and T. eremita were extracted with 70% ethanol using ultrasound assisted extraction. The qualitative and quantitative analyses of the 70% ethanol extracts were determined using the liquid chromatography-detection-ESI-mass spectrometry-(MS)/MS technique. The study of the antimicrobial activity of these extracts was performed for eight strains of Gram-positive bacteria, six strains of Gram-negative bacteria, and four cultures of fungi.

RESULTS: Chromatographic analysis of hydroalcoholic extracts of both investigated Thymus species showed very similar phenolic compounds composition. In both cases, the major components are luteolin-7-O-glucoside and rosmarinic acid. About 70% ethanol extracts of T. rasitatus and T. eremita have a broad spectrum of antimicrobial activity, exhibit the bactericidal or bacteriostatic activity against all tested bacteria and fungi at concentration range of 0.0195–20 mg/ml, but differ in their potency against tested strains of microorganisms.

CONCLUSION: About 70% ethanol extracts of T. rasitatus and T. eremita, endemic plants in the flora of Kazakhstan, can be considered as potential drugs with a wide spectrum of antimicrobial activity. The results of chromatographic analysis could be used for drug standardization.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Republic of Kazakhstan. State Pharmacopoeia of the Republic of Kazakhstan. Vol. 2. Astana: Republic of Kazakhstan; 2009. p. 802. https://doi.org/10.31082/1728-452x-2019-200-2-8-12 DOI: https://doi.org/10.31082/1728-452X-2019-200-2-8-12

Orazbaeva PZ, Akhmetalimova AM, Ivasenko SA, Loseva IV. Distribution of Some Plants of the Genus Thyme in the Territory of Central Kazakhstan. Moscow: Materials of the V Scientific- Practical Conference Modern Aspects of Using of Plant Raw Materials and Raw Materials of Natural Origin in Medicine; 2017. p. 170-2.

Atazhanova GA. Essential oils from plants of the genus Thymus L. of Kazakhstan flora: Chemical composition and prospects of application. News of the National Academy of Sciences of the Republic of Kazakhstan. Ser Biol Med 2018;5(329):45-57. https://doi.org/10.32014/2018.2518-1629.6 DOI: https://doi.org/10.32014/2018.2518-1629.6

Sadyrbekov DT, Ryazantsev OG, Kenesov BN. To Composition of Essential Oils of Some Endemic Thyme. Almaty: Materials of the V International Scientific Conference Innovative Development and Demand for Science in Modern Kazakhstan. Part 3: Natural and Technical Sciences, November 24-25; 2011. p. 111-4.

Atazhanova GA. Terpenoids from Plant Essential Oils of the Family Lamiaceae Growing in Kazakhstan. Białystok, Poland: The 24th Conference on Isoprenoids, September 9-12; 2018. p. 137.

Torina AK, Bisenova GN, Shegebaeva AA, Ibraeva A, Ryazantsev O, Atazhanova GA. Antimicrobial activity of the main components of essential oils and some of their derivatives. Bull Sci Kazakh Agrotechnical Univ. 2014;3(82):54-62.

Utegenova GA, Pallister KB, Voyich JM, Ozek G, Ozek T, Kirpotina LN, et al. Antibacterial activity of essential oils from some Artemisia and Thymus species against methicillin-resistant Staphylococcus aureus. Vestnik KazNU. Ser Biol. 2017;2(71):116-24. https://doi.org/10.3390/molecules23071679 DOI: https://doi.org/10.3390/molecules23071679

Atazhanova GA. Isoprenoid Compounds (Polyprenols, Mono and Sesquiterpenoids of Essential Oils) of Plants of Kazakhstan’s Flora. The 23rd Conference on Isoprenoids, 4-7 September 2016. p. 63-5.

Akhmetalimova AM, Marchenko AB, Ishmuratova МY, Poleszak E, Ludwiczuk A, Loseva IV. The study of the chemical composition of Thymus eremita Klok. and Thymus rasitatus Klok. from the Karaganda region. News Natl Acad Sci Republic Kazakhstan Ser Chem Technol. 2018;5(431):20-5. https://doi.org/10.32014/2018.2518-1491.3 DOI: https://doi.org/10.32014/2018.2518-1491.3

Ivasenko S, Orazbayeva P, Skalicka-Wozniak K, Ludwiczuk A, Marchenko A, Ishmuratova M, et al. Antimicrobial activity of ultrasonic extracts of two chemotypes of Thymus serpyllum L. of Central Kazakhstan and their polyphenolic profiles. Open Access Maced J Med Sci 2021;9:61-7. https://doi.org/10.3889/oamjms.2021.5520 DOI: https://doi.org/10.3889/oamjms.2021.5520

Tejchman W, Korona-Glowniak I, Malm A, Zylewski M, Suder P. Antibacterial properties of 5-substituted derivatives of rhodanine- 3-carboxyalkyl acids. Med Chem Res 2017;26:1316-24. https://doi.org/10.1007/s00044-017-1852-7 DOI: https://doi.org/10.1007/s00044-017-1852-7

Malm A, Glowniak-Lipa A, Korona-Glowniak I, Baj T. Anti- Helicobacter pylori activity in vitro of chamomile flowers, coneflower herbs, peppermint leaves and thyme herbs a preliminary report. Curr Issues Pharm Med Sci. 2015;28(1):30- 2. https://doi.org/10.1515/cipms-2015-0038 DOI: https://doi.org/10.1515/cipms-2015-0038

French GL. Bactericidal agents in the treatment of MRSA infections-the potential role of daptomycin. J Antimicrob Chemother 2006;58(6):1107-17. PMid:17040922 DOI: https://doi.org/10.1093/jac/dkl393