Differences in the Expression of β-Catenin Nucleus/Cytoplasm Ratio e-Cadherin and n-Cadherin and Correlation of β-Catenin Cytoplasm and Cadherin in Model of Duke D Stage Colorectal Cancer Cell Line

Winarko Winarko; Pudji  Rahayu; Djoko Soeatmadji; Karyono  Mintaroem

doi:10.3889/oamjms.2021.6140

Authors

Winarko Winarko Doctoral Program, Faculty of Medicine, Brawijaya University, Malang, Indonesia
Pudji Rahayu Department of Otorhinolaryngology, Faculty of Medicine, Brawijaya University, Malang, Indonesia
Djoko Soeatmadji Department of Internal Medicine, Division of Endocrinology and Metabolic, Faculty of Medicine, Brawijaya University, Malang, Indonesia
Karyono Mintaroem Department of Pathology Anatomy, Faculty of Medicine, Brawijaya University, Malang, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.6140

Keywords:

β-catenin, Colorectal cancer, E-cadherin, Fluorescein-5-isothiocyanate staining, N-cadherin

Abstract

BACKGROUND: β-catenin has a critical role in the homeostasis processes. Wnt/β-catenin signaling mainly involved in the modulation of cancer cell development.

AIM: This research aimed to investigate the differences of β-catenin expression observed in the form of nucleus–cytoplasm ratio, the differences of E-cadherin and N-cadherin expressions, and the correlation between N-cadherin and E-cadherin and β-catenin cytoplasm in Dukes D stage colorectal cancer (CRC), which is an advanced stage and has experienced metastasis.

MATERIALS AND METHODS: This study followed an experimental research design. The processes of culture manufacturing and subculture preparation of Dukes D stage CRC cell line model were performed before the administration of β-catenin, E-cadherin, and N-cadherin antibodies. The next process was staining using fluorescein-5-isothiocyanate and rhodamine, and observations were performed using a confocal microscope. The number of cells was counted, and the intensity of antibody expression based on the arbitrary unit was measured.

RESULTS: There was a significant difference between the expression of β-catenin nucleus and cytoplasm expression (p = 0.00), as well as between E-cadherin expression and N-cadherin expression (p = 0.00). In addition, a correlation also existed between an increased N-cadherin expression and decreased E-cadherin expression and β-catenin cytoplasm in Dukes D stage CRC, but the results were not significant (p = 0.837 and p = 0.108).

CONCLUSION: In advanced-stage CRC (Dukes D), the Wnt signaling proved to be active and was characterized by a high expression of β-catenin nucleus, it activates the target gene. Similarly, at the Dukes D stage, N-cadherin expression increased whereas E-cadherin expression decreased in which causing the translocation of β-catenin into the nucleus.

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