Glutathione Peroxidase-1 Pro198Leu Variant in Tuberculosis-infected Type2 Diabetes Mellitus Patients at Pulmonary Polyclinic Medan

Nurfida Khairina  Arrasyid; Milahayati Daulay; Mutiara Indah Sari

doi:10.3889/oamjms.2021.6169

Authors

Nurfida Khairina Arrasyid Department of Parasitology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Milahayati Daulay Department of Physiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Mutiara Indah Sari Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.6169

Keywords:

Glutathione peroxidase-1, Pro198Leu, Type 2 diabetes mellitus, Tuberculosis

Abstract

BACKGROUND: Glutathione peroxidase-1 (GPx-1) is an antioxidant that plays an important to the body protection system against oxidative stress. The GPx-1 polymorphism that has been identified in individual with several diseases.

AIM: This study aimed to observe the distribution of GPx-1 Pro198Leu variant in tuberculosis (TB)-infected Type 2 diabetes mellitus (T2DM) patients at pulmonary polyclinic Medan. GPx-1 Pro198Leu polymorphism was genotyped in 40 T2DM patients that also infected by TB.

MATERIALS AND METHODS: Analysis of GPx-1 Pro198Leu polymorphism was done using polymerase chain reaction (PCR) and restriction fragment length polymorphism. The PCR products were digested 4 h at 37°C with Apa1 restriction enzyme. The result of Apa1 enzyme digestion was visualized with 4% agarose.

RESULTS: From 40 TB-infected T2DM patients, the frequency of genotypes CC,CT, and TT were, respectively, 82.5%, 17.5%, and 0%. The frequency of C allele was higher than T allele, i.e. 91.3% and 8.7%.

CONCLUSION: It was concluded that in TB-infected T2DM patients at pulmonary polyclinic Medan, the GPx-1 Pro198Leu polymorphism has CC variant higher than CT, whereas the TT genotype was not found. The frequency of the C allele is higher than the T allele.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organ J. 2012;5(1):9-19. https://doi.org/10.1097/wox.0b013e3182439613 PMid:23268465 DOI: https://doi.org/10.1097/WOX.0b013e3182439613

Khan AA, Rahmani AH, Aldebasi YH, Aly SM. Biochemical and pathological studies on peroxidases-an updated review. Glob J Health Sci. 2014;6(5):87-98. https://doi.org/10.5539/gjhs.v6n5p87 PMid:25168993 DOI: https://doi.org/10.5539/gjhs.v6n5p87

Ismail S, Essawi M. Genetic polymorphism studies in humans. Middle East J Med Genet. 2012;1(2):57-63. https://doi.org/10.1097/01.mxe.0000415225.85003.47 DOI: https://doi.org/10.1097/01.MXE.0000415225.85003.47

Crawford A, Fassett R, Geraghty D, Kunde D, Ball M, Robertson I, et al. Relationships between single nucleotide polymorphisms of antioxidant enzymes and disease. Gene. 2012;501(2):89-103. https://doi.org/10.1016/j.gene.2012.04.011 PMid:22525041 DOI: https://doi.org/10.1016/j.gene.2012.04.011

Banerjee M, Vats P. Reactive metabolites and antioxidant gene polymorphisms in Type 2 diabetes mellitus. Indian J Hum Genet. 2014;20(1):10-9. https://doi.org/10.4103/0971-6866.132747 PMid:24959009 DOI: https://doi.org/10.4103/0971-6866.132747

Sari MI, Daulay M, Wahyuni DD. Superoxide dismutase levels and polymorphism (Ala16val) in tuberculosis patients with diabetes mellitus in Medan city. Open Access Maced J Med Sci. 2019;7(5):730-5. https://doi.org/10.3889/oamjms.2019.195 PMid:30962829 DOI: https://doi.org/10.3889/oamjms.2019.195

Saravani S, Miri HR, Saravani R, Yari D, Nakhaee A, Mahjoubifard M. Association of catalase (rs7943316) and glutathione peroxidase-1 (rs1050450) polymorphisms with the risk of Type 2 diabetes (T2DM). Mol Genet Microbiol Virol. 2015;30:216-20. https://doi.org/10.3103/s0891416815040096 DOI: https://doi.org/10.3103/S0891416815040096

Guerrero CH, Chávez PH, Castro NM, Carriedo AP, Lizaur AP, Del Río SG. Glutathione peroxidase-1 Pro200Leu polymorphism (rs1050450) is associated with morbid obesity independently of the presence of prediabetes or diabetes in women from central Mexico. Nutr Hosp. 2015;32(4):1516-25. PMid:26545512

Buraczynska M, Buraczynska K, Dragan M, Ksiazek A. Pro198Leu polymorphism in the glutathione peroxidase 1 gene contributes to diabetic peripheral neuropathy in Type 2 diabetes patients. Neuromolecular Med. 2017;19(1):147-53. https://doi.org/10.1007/s12017-016-8438-2 PMid:27592002 DOI: https://doi.org/10.1007/s12017-016-8438-2

Perkumpulan Endokrinologi Indonesia (Perkeni). Konsensus Pengelolaan dan Pencegahan Diabetes Melitus Tipe 2 di Indonesia; 2015. Available from: https://www.pbperkeni.or.id/ wp-content/uploads/2019/01/4.-Konsensus-Pengelolaan-dan-Pencegahan-Diabetes-melitus-tipe-2-di-Indonesia- PERKENI-2015.pdf. [Last accessed on 2021 Mar 10]. https:// doi.org/10.23886/ejki.3.4810

Baghaei P, Marjani M, Javanmard P, Tabarsi P, Masjedi MR. Diabetes mellitus and tuberculosis facts and controversies. J Diabetes Metab Disord. 2013;12(1):58. https://doi.org/10.1186/2251-6581-12-58 PMid:24360398 DOI: https://doi.org/10.1186/2251-6581-12-58

Rao S, Rahim M, Iqbal K, Haroon F, Hasan Z. Impact of diabetes on mechanisms of immunity against Mycobacterium tuberculosis. J Pak Med Assoc. 2019;69(1):94-8. PMid:30623920

Narasimhan P, Wood J, Macintyre CR, Mathai D. Risk factors for tuberculosis. Pulm Med. 2013;2013:828939. https://doi.org/10.1155/2013/828939 PMid:23476764 DOI: https://doi.org/10.1155/2013/828939

Alisjahbana B, Van Crevel R, Sahiratmadja E, Den Heijer M, Maya A, Istriana E, et al. Diabetes mellitus is strongly associated with tuberculosis in Indonesia. Int J Tuberc Lung Dis. 2006;10(6):696-700. https://doi.org/10.5588/09640569513002 PMid:16776459 DOI: https://doi.org/10.5588/09640569513002

Yuniastuti A, Susanti R. Analisis sekuen gen glutation peroksidase (GPX1) sebagai deteksi stres oksidatif akibat infeksi Mycobacterium Tuberculosis. Sainteknol. 2013;11:103-12.

Sari MI, Syarifah S, Daulay M, Tala ZZ. The association of antioxidants gene polymorphisms (SOD2 Ala16Val, GPx1 Pro198Leu, GSTP1 Ile105Val, and Cat-21 A/T) and risk of Type 2 diabetes mellitus. Open Access Maced J Med Sci. 2020;8:781-6. https://doi.org/10.3889/oamjms.2020.5397 DOI: https://doi.org/10.3889/oamjms.2020.5397

Tang TS, Prior SL, Li KW, Ireland HA, Bain SC, Hurel SJ, et al. Association between the rs1050450 glutathione peroxidase-1 (C>T) gene variant and peripheral neuropathy in two independent samples of subjects with diabetes mellitus. Nutr Metab Cardiovasc Dis. 2012;22(5):417-25. https://doi.org/10.1016/j.numecd.2010.08.001 PMid:21185702 DOI: https://doi.org/10.1016/j.numecd.2010.08.001

Venter JC, Adams MD, Myers EW, Li PW, Mural RJ, Sutton GG. The sequence of the human genome. Science. 2001;291(5507):1304-51. https://doi.org/10.1126/ science.1058040 PMid:11181995

Sari MI, Tala ZZ, Wahyuni DD. Association between glycated hemoglobin with the levels of serum proinflammatory cytokines and antioxidants in patients with Type 2 diabetes mellitus in universitas Sumatera Utara hospital. Open Access Maced J Med Sci. 2019;7(5):715-20. https://doi.org/10.3889/oamjms.2019.168 PMid:30962826 DOI: https://doi.org/10.3889/oamjms.2019.168