Glutathione Peroxidase-1 Pro198Leu Variant in Tuberculosis-infected Type2 Diabetes Mellitus Patients at Pulmonary Polyclinic Medan

Authors

  • Nurfida Khairina Arrasyid Department of Parasitology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Milahayati Daulay Department of Physiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Mutiara Indah Sari Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.6169

Keywords:

Glutathione peroxidase-1, Pro198Leu, Type 2 diabetes mellitus, Tuberculosis

Abstract

BACKGROUND: Glutathione peroxidase-1 (GPx-1) is an antioxidant that plays an important to the body protection system against oxidative stress. The GPx-1 polymorphism that has been identified in individual with several diseases.

AIM: This study aimed to observe the distribution of GPx-1 Pro198Leu variant in tuberculosis (TB)-infected Type 2 diabetes mellitus (T2DM) patients at pulmonary polyclinic Medan. GPx-1 Pro198Leu polymorphism was genotyped in 40 T2DM patients that also infected by TB.

MATERIALS AND METHODS: Analysis of GPx-1 Pro198Leu polymorphism was done using polymerase chain reaction (PCR) and restriction fragment length polymorphism. The PCR products were digested 4 h at 37°C with Apa1 restriction enzyme. The result of Apa1 enzyme digestion was visualized with 4% agarose.

RESULTS: From 40 TB-infected T2DM patients, the frequency of genotypes CC,CT, and TT were, respectively, 82.5%, 17.5%, and 0%. The frequency of C allele was higher than T allele, i.e. 91.3% and 8.7%.

CONCLUSION: It was concluded that in TB-infected T2DM patients at pulmonary polyclinic Medan, the GPx-1 Pro198Leu polymorphism has CC variant higher than CT, whereas the TT genotype was not found. The frequency of the C allele is higher than the T allele.

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Published

2021-06-27

How to Cite

1.
Arrasyid NK, Daulay M, Sari MI. Glutathione Peroxidase-1 Pro198Leu Variant in Tuberculosis-infected Type2 Diabetes Mellitus Patients at Pulmonary Polyclinic Medan. Open Access Maced J Med Sci [Internet]. 2021 Jun. 27 [cited 2021 Sep. 20];9(A):403-6. Available from: https://oamjms.eu/index.php/mjms/article/view/6169

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