Exosomes of Adipose-derived Stem Cells Conditioned Media Promotes Retinoblastoma and Forkhead-Box M1 Protein Expression

Sinta Murlistyarini; Lulus Putri Aninda; Sri Widyarti; Agustina Tri Endharti; Teguh Wahju Sardjono

doi:10.3889/oamjms.2021.6195

Authors

Sinta Murlistyarini Doctoral Program of Medical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia; Department of Dermatology and Venereology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia https://orcid.org/0000-0001-5706-8610
Lulus Putri Aninda Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
Sri Widyarti Doctoral Program of Medical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia; Department of Biology, Faculty of Mathematics and Natural Science, Universitas Brawijaya, Malang, Indonesia; https://orcid.org/0000-0002-9100-7544
Agustina Tri Endharti Doctoral Program of Medical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia; Department of Parasitology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia; Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia https://orcid.org/0000-0002-2062-5740
Teguh Wahju Sardjono Doctoral Program of Medical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia; Department of Parasitology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.6195

Keywords:

Cellular senescence, Exosome, Adipose-derived stem cell-conditioned medium, Human dermal fibroblast, Retinoblastoma protein, Forkhead Box M1 proteinForkhead Box M1 protein

Abstract

BACKGROUND: In the senescence process, the retinoblastoma (Rb) protein binds to E2F in hypophosphorylated conditions, preventing the cell to enter the S-phase in the cell cycle. Human Forkhead Box M1 (FOXM1) protein, key regulator G1/S and G2/M phases, decreases in the senescence process. Many studies have been carried out to reverse this system, one of which used exosomes of adipose-derived stem c ells conditioned media (ADSC-CM). These exosomes contain a variety of specific proteins which have pro-proliferation properties, however, little is known on the role of these exosomes toward the change of phosphorylated Rb and FOXM1.

AIM: This study aims to find out the involvement of exosomes of ADSC-CM on these two proteins on senescence human dermal fibroblasts (HDFs).

METHODS: In vitro experiment was undergone randomization sample and non-blinded pre-/post-test control group. The primary culture of senescent HDFs was transfected with exosomes of ADSC-CM; then, its effect on migration and senescence reversal was observed through analyzing Sa-β-gal, Rb, and FOXM1 protein expression.

RESULTS: The expression of Sa-β-gal was higher in the control group. Our result demonstrated the exosome of ADSC-CM significantly induced the expression of Rb and FOXM1 protein in senescent HDFs (p < 0.05).

CONCLUSION: It proved that exosomes of ADSC-CM could shift the senescent fibroblast into metabolically active cells.

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