Expression of Cytokeratin 6 and 16 in Middle Ear Cholesteatoma

Jacky Munilson; Yan  Edward; Lorensia  Fitra Dwita; Hirowati  Ali

doi:10.3889/oamjms.2021.6248

Authors

Jacky Munilson Department of Otorhinolaryngology, Faculty of Medicine, Andalas University, Padang, Indonesia https://orcid.org/0000-0003-4116-8777
Yan Edward Department of Otorhinolaryngology, Faculty of Medicine, Andalas University, Padang, Indonesia
Lorensia Fitra Dwita Department of Otorhinolaryngology, Faculty of Medicine, Andalas University, Padang, Indonesia https://orcid.org/0000-0003-1682-8673
Hirowati Ali Department of Biochemistry, Faculty of Biomedics, Andalas University, Padang, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.6248

Keywords:

Cholesteatoma, Cytokeratin 6, Cytokeratin 16, Expression, Middle ear

Abstract

BACKGROUND: Cholesteatoma is hyperproliferative because of the response of direct biomechanical trauma, and inflammation processes then lead to temporal bone destruction with some clinical manifestations of complications. The hyperproliferation mechanism occurred because of the activation of intermediate filament protein type I and type II known as cytokeratin (CK).

AIM: This study aimed to examine the expression CK 6 and CK 16 in cholesteatoma.

METHODS: This is a cross-sectional comparative study. Cholesteatoma specimens obtained from 15 patients who underwent surgery were considered as the case, and 15 normal retro-auricular skins were considered as the control. All samples were examined for expression through immunohistochemistry and scored using the immunoreactivity score. Data were analyzed using SPSS via χ²test, and the difference was significant (p < 0.05).

RESULTS: The expression of CK 6 was high in cholesteatoma (33.3%) and low in retro-auricular skin. The expression of CK 16 was high in all samples of cholesteatoma and mostly high in the retro-auricular skin; both expressions were statistically significant (p < 0.05).

CONCLUSION: The expression of CK 6 and CK 16 in cholesteatoma was higher than in normal retro-auricular skin.

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References

World Health Organization. Chronic Suppurative Otitis Media: Burden of Illness and Management Options. Geneva: World Health Organization; 2004. Available from: https://apps.who.int/iris/handle/10665/42941 [Last accessed on 2021 May 17].

Utami TF, Sudarman K, Rianto BU, Christanto A. Rinitis Alergi sebagai Faktor Risiko Otitis Media Supuratif Kronis [Allergic Rhinitis as a Risk Factor for Chronic Suppurative Otitis Media]. Cermin Dunia Kedokt. 2010;179:425-9.

Darmawan AB, Anjarwati DU. Perbedaan sensitivitas tetes telinga antibiotik terhadap Pseudomonas aeruginosa pada otitis media supuratif kronik [Differences in the sensitivity of antibiotic ear drops to Pseudomonas aeruginosa in chronic suppurative otitis media]. Oto Rhino Laryngol Indones. 2012;42(2):77-82. https://doi.org/10.32637/orli.v42i2.22 DOI: https://doi.org/10.32637/orli.v42i2.22

Helmi. Otitis Media Supuratif Kronis [Chronic Suppurative Otitis Media]. In: Otitis Media Supuratif Kronis Pengetahuan Dasar Terapi Medik Mastoidektomi Timpanoplasti [Chronic Suppurative Otitis Media Basic Knowledge of Medical Therapy Mastoidectomy Tympanoplasty]. Jakarta: Balai Penerbit FKUI; 2005.

Likus W, Siemianowicz K, Markowski J, Wiaderkiewicz J, Kostrząb-Zdebel A, Jura-Szołtys E, et al. Bacterial infections and osteoclastogenesis regulators in men and women with cholesteatoma. Arch Immunol Ther Exp (Warsz). 2016;64(3):241-7. https://doi.org/10.1007/s00005-015-0373-7 DOI: https://doi.org/10.1007/s00005-015-0373-7

Maniu A, Harabagiu O, Schrepler MP, Cătană A, Fănuţă B, Mogoantă CA. Molecular biology of cholesteatoma. Rom J Morphol Embryol. 2014;55(1):7-13.

de Aquino JE, Filho NA, de Aquino JN. Epidemiology of middle ear and mastoid cholesteatomas: Study of1146 cases. Braz J Otorhinolaryngol. 2011;77(3):341-7. https://doi.org/10.1590/S1808-86942011000300012 DOI: https://doi.org/10.1590/S1808-86942011000300012

Jackler RK, Maria PL, Varsak YK, Nguyen A, Blevin NH. A new theory on the pathogenesis of acquired cholesteatoma: Mucosal traction. Laryngoscope. 2015;125(Suppl 4):S1-14. https://doi.org/10.1002/lary.25261 DOI: https://doi.org/10.1002/lary.25261

Kuo CL, Shiao AS, Yung M, Sakagami M, Sudhoff H, Wang CH, et al. Updates and knowledge gaps in cholesteatoma research. Biomed Res Int. 2015;2015:854024. https://doi.org/10.1155/2015/854024 DOI: https://doi.org/10.1155/2015/854024

Hamed MA, Nakata S, Sayed RH, Ueda H, Badawy BS, Nishimura Y, et al. Pathogenesis and bone resorption in acquired cholesteatoma: Current knowledge and future prospectives. Clin Exp Otorhinolaryngol. 2016;9(4):298-308. https://doi.org/10.21053%2Fceo.2015.01662 DOI: https://doi.org/10.21053/ceo.2015.01662

Pivarcsi A, Müller A, Hippe A, Rieker J, van Lierop A, Steinhoff M, et al. Tumor immune escape by the loss of homeostatic chemokine expression. Proc Natl Acad Sci. 2007;104(48):19055-60. DOI: https://doi.org/10.1073/pnas.0705673104

Duan L, Chen H, Gao J. The cytoskeleton of the system. Lab Cell Biol Technic 2017;1(1):17-22.

DePianto D, Coulombe PA. Intermediate filaments and tissue repair. Exp Cell Res 2004;301(1):68-76. https://doi.org/10.1016/j.yexcr.2004.08.007 DOI: https://doi.org/10.1016/j.yexcr.2004.08.007

Lessard JC, Pina-Paz S, Rotty JD, Hickerson RP, Kaspar RL, Balmainet A, et al. Keratin 16 regulates innate immunity in response to epidermal barrier breach. Proc Natl Acad Sci. 2013;110(48):19537-42. https://doi.org/10.1073/pnas.1309576110 PMid:24218583 DOI: https://doi.org/10.1073/pnas.1309576110

Juráňová J, Franková J, Ulrichová J. The role of keratinocytes in inflammation. J Appl Biomed. 2017;15(3):169-79. https://doi.org/10.1016/j.jab.2017.05.00316. Freedberg IM, Tomic-Canic M, Komine M, Blumenberg M. Keratins and the keratinocyte activation cycle. J Invest Dermatol 2001;116(5):633-40. https://doi.org/10.1046/j.1523-1747.2001.01327.x PMid:11348449 DOI: https://doi.org/10.1046/j.1523-1747.2001.01327.x

Koç A, Emre E. An overview to cytokeratin pattern. ACU Sağlık Bil Derg 2015;6(1):1-6.

Kim HJ, Tinling SP, Chole RA. Expression patterns of cytokeratins in retraction pocket cholesteatomas. Laryngoscope. 2009;111(6):1032-6. https://doi.org/10.1097/00005537-200106000-00018 DOI: https://doi.org/10.1097/00005537-200106000-00018

Klenke C, Janowski S, Borck D, Widera D, Ebmeyer J, Kalinowski J, et al. Identification of novel cholesteatoma-related gene expression signatures using full-genome microarrays. PLoS One. 2012;7(12):e52718. https://doi.org/10.1371/journal.pone.0052718 DOI: https://doi.org/10.1371/journal.pone.0052718

Olszewska E, Wagner M, Bernal-Sprekelsen M, Ebmeyer J, Dazert S, Hildmann H, et al. Etiopathogenesis of cholesteatoma. Eur Arch Otorhinolaryngol 2004;261(1):6-24. https://doi.org/10.1007/s00405-003-0623-x DOI: https://doi.org/10.1007/s00405-003-0623-x

Swanda D, Edward Y, Munilson J. Ekspresi Tumor Necrosis Factor dan Interleukin-6 Pada Kolesteatoma Penderita Otitis Media Supuratif Kronis [Expression of Tumor Necrosis Factor and Interleukin-6 in Cholesteatoma in Chronic Suppurative Media Otitis Patients]. Padang: Master Thesis, Andalas University; 2017. p. 37-9.

Kim HJ, Tinling SP, Chole RA. Increased proliferation and migration of epithelium in advancing experimental cholesteatomas. Otol Neurotol. 2002;23(6):840-4. https://doi.org/10.1097/00129492-200211000-00005 PMid:12438843 DOI: https://doi.org/10.1097/00129492-200211000-00005

Nguyen KH, Suzuki H, Ohbuchi T, Wakasugi T, Koizumi H, Hashida K, et al. Possible participation of acidic pH in bone resorption in middle ear cholesteatoma. Laryngoscope. 2014;124(1):245-50. https://doi.org/10.1002/lary.23883 PMid:24122656 DOI: https://doi.org/10.1002/lary.23883

Fedchenko N, Reifenrath J. Different approaches for interpretation and reporting of immunohistochemistry analysis results in the bone tissue a review. Diagn Pathol. 2014;9:221. https://doi.org/10.1186/s13000-014-0221-9 PMid:25432701 DOI: https://doi.org/10.1186/s13000-014-0221-9