Expression of Cytokeratin 6 and 16 in Middle Ear Cholesteatoma


  • Jacky Munilson Department of Otorhinolaryngology, Faculty of Medicine, Andalas University, Padang, Indonesia
  • Yan Edward Department of Otorhinolaryngology, Faculty of Medicine, Andalas University, Padang, Indonesia
  • Lorensia Fitra Dwita Department of Otorhinolaryngology, Faculty of Medicine, Andalas University, Padang, Indonesia
  • Hirowati Ali Department of Biochemistry, Faculty of Biomedics, Andalas University, Padang, Indonesia



Cholesteatoma, Cytokeratin 6, Cytokeratin 16, Expression, Middle ear


BACKGROUND: Cholesteatoma is hyperproliferative because of the response of direct biomechanical trauma, and inflammation processes then lead to temporal bone destruction with some clinical manifestations of complications. The hyperproliferation mechanism occurred because of the activation of intermediate filament protein type I and type II known as cytokeratin (CK).

AIM: This study aimed to examine the expression CK 6 and CK 16 in cholesteatoma.

METHODS: This is a cross-sectional comparative study. Cholesteatoma specimens obtained from 15 patients who underwent surgery were considered as the case, and 15 normal retro-auricular skins were considered as the control. All samples were examined for expression through immunohistochemistry and scored using the immunoreactivity score. Data were analyzed using SPSS via χ2 test, and the difference was significant (p < 0.05).

RESULTS: The expression of CK 6 was high in cholesteatoma (33.3%) and low in retro-auricular skin. The expression of CK 16 was high in all samples of cholesteatoma and mostly high in the retro-auricular skin; both expressions were statistically significant (p < 0.05).

CONCLUSION: The expression of CK 6 and CK 16 in cholesteatoma was higher than in normal retro-auricular skin.


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How to Cite

Munilson J, Edward Y, Fitra Dwita L, Ali H. Expression of Cytokeratin 6 and 16 in Middle Ear Cholesteatoma. Open Access Maced J Med Sci [Internet]. 2021 Oct. 14 [cited 2024 May 24];9(B):1323-8. Available from:



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