Prevalence of Soil-transmitted Helminths Infection in Students of Klungkung, Bali, after Mass Treatment with AlbendazolePrevalence of Soil-transmitted Helminths Infection in Students of Klungkung, Bali, after Mass Treatment with Albendazole

Putu Indah Budiapsari; I. Kadek Swastika; Sri Masyeni

doi:10.3889/oamjms.2021.6266

Authors

Putu Indah Budiapsari Department of Parasitology, Faculty of Medicine and Health Sciences Warmadewa University, Denpasar, Bali, Indonesia
I. Kadek Swastika Department of Parasitology, Faculty of Medicine, Udayana University, Denpasar, Bali, Indonesia
Sri Masyeni Department of Internal Medicine, Faculty of Medicine and Health Sciences, Warmadewa University, Denpasar, Bali, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.6266

Keywords:

Children, Helminth, Molecular, Soil, Transmitted

Abstract

The success of the anti-helminth mass treatment use Albendazole makes detection of soil transmitted helminth infections even more difficult to do microscopically. It is hoped that the molecular method was able to help increase the detectability of Soil Transmitted Helminth infection. The research aimed is to evaluate effectiveness of Albendazole administration in Bali, to identify the presence of β-tubulin gene as molecular diagnosis of STHs infection among children who treated by Albendazole. This study is a cross-sectional study that recruits elementary school children aged 6-12 years as subjects. Stool examination was carried out using the Kato-Katz technique, then followed by a molecular method using the B-tubulin gene as the target gene. The results showed that only 1 sample out of 140 examined using Kato-Katz was positive for Trichuris trichiura. 30 samples were then extracted from the faeces and performed Polymerase Chain Reaction. A total of 4 positive samples detected the B-tubulin Ascaris lumbricoides gene and 1 positive sample of the B-tubulin Trichuris trichiura gene. In conclusion, albendazole is still effective to treat STH infection, the molecular method has a higher detectability than the microscopic method.

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References

Abraham D, Kaliappan SP, Walson JL, Rao Ajjampur SS. Intervention strategies to reduce the burden of soil-transmitted

helminths in India. Indian J Med Res. 2018;147(6):533-44. https://doi.org/10.4103/ijmr.ijmr_881_18 PMid:30168484 DOI: https://doi.org/10.4103/ijmr.IJMR_881_18

Apsari PI, Arwati H, Dachlan YP. Correlation of eosinofil and basophil count with intensity of soil transmitted helminth infection among farmers in bali. Iop Conf Ser. 2018;434(1):012142. https://doi.org/10.1088/1757-899x/434/1/012142 DOI: https://doi.org/10.1088/1757-899X/434/1/012142

Apsari PI, Arwati H, Dachlan YP. Correlation of total IgE level and intensity of infection among soil transmitted helminthiasis farmers in Klungkung regency, Bali, Indonesia. Folia Med Indones. 2019;55(2): 93-9. https://doi.org/10.20473/fmi.v55i2.14333 DOI: https://doi.org/10.20473/fmi.v55i2.14333

Apsari PI, Indraningrat AA, Arwati H, Dachlan Y. Short communication: Prevalence and risk factors of soil-transmitted helminth infection among farmers in gelgel village, Klungkung district, Bali, Indonesia. Biodiversitas. 2020;21(4):1535-40. https://doi.org/10.13057/biodiv/d210434 DOI: https://doi.org/10.13057/biodiv/d210434

Barda B, Schindler C, Wampfler R, Ame S, Ali SM, Keiser J. Comparison of real-time PCR and the Kato-Katz method for the diagnosis of soil-transmitted helminthiasis and assessment of cure in a randomized controlled trial. BMC Microbiol. 2020;20:298. https://doi.org/10.1186/s12866-020-01963-9 DOI: https://doi.org/10.1186/s12866-020-01963-9

Becker SL, Liwanag HJ, Snyder JS, Akogun O, Belizario V, Freeman MC, et al. Toward the 2020 goal of soil-transmitted helminthiasis control and elimination. PLoS Negl Trop Dis. 2018;12(8):e0006606. https://doi.org/10.1371/journal.pntd.0006606 PMid:30106975 DOI: https://doi.org/10.1371/journal.pntd.0006606

Brooker SJ. Soil-transmitted helminth treatment: Multiple-drug regimens. Lancet Infect Dis. 2018;18(7):698-9. https://doi.org/10.1016/s1473-3099(18)30268-8 PMid:29673736 DOI: https://doi.org/10.1016/S1473-3099(18)30268-8

Benjamin-Chung J, Pilotte N, Ercumen A, Grant JR, Maasch JR, Gonzalez AM, et al. Comparison of multi-parallel qPCR and double-slide Kato-Katz for detection of soil-transmitted helminth infection among children in rural Bangladesh. PLoS Negl Trop Dis. 2020;14(4):e0008087. https://doi.org/10.1371/journal.pntd.0008087 PMid:32330127 DOI: https://doi.org/10.1371/journal.pntd.0008087

Betson M, Nejsum P, Bendall RP, Deb RM, Stothard JR. Molecular epidemiology of ascariasis: A global perspective on the transmission dynamics of Ascaris in people and pigs. J Infect Dis. 2014;210(6):932-41. https://doi.org/10.1093/infdis/jiu193 PMid:24688073 DOI: https://doi.org/10.1093/infdis/jiu193

Diawara A, Halpenny CM, Churcher TS, Mwandawiro C, Kihara J, Kaplan RM, et al. Association between response to albendazole treatment and β-tubulin genotype frequencies in soil-transmitted. PLoS Negl Trop Dis. 2013;7(5):e2247. https://doi.org/10.1371/journal.pntd.0002247 PMid:23738029 DOI: https://doi.org/10.1371/journal.pntd.0002247

Jia TW, Melville S, Utzinger J, King CH, Zhou XN. Soil-transmitted helminth reinfection after drug treatment: A systematic review and meta-analysis. PLoS Negl Trop Dis. 2012;6(5):e1621. https://doi.org/10.1371/journal.pntd.0001621 PMid:22590656 DOI: https://doi.org/10.1371/journal.pntd.0001621

Krücken J, Fraundorfer K, Mugisha JC, Ramünke S, Sifft KC, Geus D, et al. Reduced efficacy of albendazole against Ascaris lumbricoides in Rwandan schoolchildren. Int J Parasitol Drugs Drug Resist. 2017;7(3):262-71. https://doi.org/10.1016/j.ijpddr.2017.06.001 PMid:28697451 PMid:28697451 DOI: https://doi.org/10.1016/j.ijpddr.2017.06.001

Matamoros G, Rueda MM, Rodríguez C, Gabrie JA, Canales M, Fontecha G, et al. High endemicity of soil-transmitted helminths in a population frequently exposed to albendazole but no evidence of antiparasitic resistance. Trop Med Infect Dis. 2019;4(2):73. https://doi.org/10.3390/tropicalmed4020073 PMid:31035610 DOI: https://doi.org/10.3390/tropicalmed4020073

Moser W, Ali SM, Ame SM, Speich B, Puchkov M, Huwyler J, et al. Efficacy and safety of oxantel pamoate in school-aged children infected with Trichuris trichiura on Pemba Island, Tanzania: A parallel, randomised, controlled, dose-ranging study. Lancet Infect Dis. 2016;16(1):53-60. https://doi.org/10.1016/s1473-3099(15)00271-6 PMid:26388169 DOI: https://doi.org/10.1016/S1473-3099(15)00271-6

Moser W, Sayasone S, Xayavong S, Bounheuang B, Puchkov M, Huwyler J, et al. Efficacy and tolerability of triple drug therapy with albendazole, pyrantel pamoate, and oxantel pamoate compared with albendazole plus oxantel pamoate, pyrantel pamoate plus oxantel pamoate, and mebendazole plus pyrantel pamoate and oxantel pamoate against hookworm infections in school-aged children in Laos: A randomised, single-blind trial. Lancet Infect Dis. 2018;18(7):729-37. https://doi.org/10.1016/s1473-3099(18)30220-2 PMid:29673735 DOI: https://doi.org/10.1016/S1473-3099(18)30220-2

Mutombo PN, Man NWY, Nejsum P, Ricketson R, Gordon CA, Robertson G, et al. Diagnosis and drug resistance of human soil-transmitted helminth infections: A public health perspective. Adv Parasitol. 2019;104:247-326. https://doi.org/10.1016/bs.apar.2019.02.004 PMid:31030770 DOI: https://doi.org/10.1016/bs.apar.2019.02.004

Ngwese MM, Manouana GP, Moure PA, Ramharter M, Esen M, Adégnika AA. Diagnostic techniques of soil-transmitted helminths: Impact on control measures. Trop Med Infect Dis. 2020;5(2):93. https://doi.org/10.3390/tropicalmed5020093 PMid:32516900 DOI: https://doi.org/10.3390/tropicalmed5020093

O’Connell EM, Nutman TB. Molecular diagnostics for soil-transmitted helminths. Am J Trop Med Hyg. 2016;95(3):508-13. PMid:27481053 DOI: https://doi.org/10.4269/ajtmh.16-0266

Rashwan N, Scott M, Prichard R. Rapid genotyping of β-tubulin polymorphisms in Trichuris trichiura and Ascaris lumbricoides. PLoS Negl Trop Dis. 2017;11(1):e0005205. https://doi.org/10.1371/journal.pntd.0005205 PMid:28081124 DOI: https://doi.org/10.1371/journal.pntd.0005205

Sinniah B, Hassan AK, Sabariah I, Soe MM, Ibrahim Z, Ali O. prevalence of Intestinal parasitic Infections among communities living in different habitats and its comparison with one hundred and one studies conducted over the past 42 years (1970-2013) in Malaysia. Trop Biomed. 2014;31(2):190-206. PMid:25134888

Speich B, Ame SM, Ali SM, Alles R, Hattendorf J, Utzinger J, et al. Efficacy and safety of nitazoxanide, albendazole, and nitazoxanide albendazole against Trichuris trichiura infection: A randomized controlled trial. PLoS Negl Trop Dis. AQ6 2012;6(6):e1685. https://doi.org/10.1371/journal.pntd.0001685 PMid:22679525 DOI: https://doi.org/10.1371/journal.pntd.0001685

Sudarmaja I. Epidemologi Infeksi Kecacingan di Bali, Bali: Laboratorium Parasitologi Universitas Udayana; 2011.

Vercruysse J, Albonico M, Behnke JM, Kotze AC, Prichard RK, McCarthy JS, et al. Is anthelmintic resistance a concern for the control of human soil-transmitted helminths? Int J Parasitol. 2011;1:14-27. https://doi.org/10.1016/j.ijpddr.2011.09.002 DOI: https://doi.org/10.1016/j.ijpddr.2011.09.002

Vercruysse J, Behnke JM, Albonico M, Ame SM, Angebault C, Bethony JM, et al. Assessment of the anthelmintic efficacy of albendazole in school children in seven countries where soil-transmitted helminths are endemic. PLoS Negl Trop Dis. 2011;5(3):e948. https://doi.org/10.1371/journal.pntd.0000948 PMid:21468309 DOI: https://doi.org/10.1371/journal.pntd.0000948

Welch VA, Ghogomu E, Hossain A, Awasthi S, Bhutta ZA, Cumberbatch C, et al. Mass deworming to improve developmental health and wellbeing of children in low-income and middle-income countries: A systematic review and network meta-analysis. Lancet Glob Health. 2017;5(1):e40-50. https://doi.org/10.1016/s2214-109x(16)30242-x PMid:27955788 DOI: https://doi.org/10.1016/S2214-109X(16)30242-X

World Health Organization; 2017. Available from: https://www. who.int/intestinal_worms. [Last accessed on 2017 Aug 16].

Yuliana Y, Djuardi Y, Supali T. The profile of codon 200 β-tubulin gene of Ascaris lumbricoides L. and Trichuris trichiura L. from infected people in Nangapanda Sub-District, East Nusa Tenggara. Med J Indones. 2018;27(4):304-9. https://doi.org/10.13181/mji.v27i4.1935 DOI: https://doi.org/10.13181/mji.v27i4.1935