The Effect of Ophiocephalus striatus sp. Extract on Nitric Oxide in Ischemic Stroke Model

Iskandar Nasution; Hasan Sjahrir; Syafruddin Ilyas; Muhammad Ichwan

doi:10.3889/oamjms.2021.6299

Authors

Iskandar Nasution Department of Neurology, Faculty of Medicine, University of Sumatera Utara, Haji Adam Malik General Hospital, Medan, Indonesia https://orcid.org/0000-0003-1342-0613
Hasan Sjahrir Department of Neurology, Faculty of Medicine, University of Sumatera Utara, Haji Adam Malik General Hospital, Medan, Indonesia
Syafruddin Ilyas Department of Biology, Faculty of Mathematics and Natural Science, University of Sumatera Utara, Medan, Indonesia
Muhammad Ichwan Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Sumatera Utara, Haji Adam Malik General Hospital, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.6299

Keywords:

Cerebral angiogenesis, Ophiocephalus striatus sp. extract, Nitric oxide, Ischemic stroke

Abstract

BACKGROUND: One of alternative medicine in stroke therapy is Ophiocephalus striatus sp. extract. The nutrients contained in the O. striatus sp. extract, namely amino acids, fatty acids, cuprum, and zinc, are useful for the process of angiogenesis in poststroke patients through increased endothelial nitric oxide synthase.

AIM: We hypothesized that there was an effect of giving O. striatus sp. extract to cerebral angiogenesis process of Sprague Dawley rats ischemic stroke models through the level of NO.

METHODS: This was evidenced by conducting experimental studies on rats ischemic stroke models which were divided into five groups, (a) K (−) group (no ligation, no treatment), (b) K (+) group (ligation, no treatment), (c) P1 group (ligation, 200 mg extract), (d) P2 group (ligation, 400 mg extract), and (e) P3 group (ligation, 800 mg extract). Then blood sample was taken on day 3 to assess levels of NO.

RESULTS: There was increased level of NO in P1 (p = 0.001), P2 (p < 0.001), and P3 (p < 0.001) groups compared to K (+) group. The level of NO increases along with the increasing dose of O. striatus sp. extract. Histological examination revealed that there was formation of new blood vessel in the P1, P2, and P3 groups compared to K (+) group.

CONCLUSION: Our study showed that O. striatus sp. extract improves cerebral angiogenesis in rat models of ischemic stroke.

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