The Use of 25-hydroxyvitamin D Saliva Test to Replace Vitamin D Serum Blood Test in Healthy People


  • Dina Keumala Sari Tropical Medicine Program Study, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Liza Meutia Sari Department of Oral Medicine, Faculty of Dentistry, Universitas Syiah Kuala, Banda Aceh, Indonesia
  • Lidya Imelda Laksmi Department of Anatomy Pathology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Farhat Farhat Department of Ear, Nose, Throat, Head, and Neck, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia



Vitamin D, Cross-sectional, Non-invasive, Correlation, Deficiency


BACKGROUND: Routine examination of Vitamin D levels is carried out by checking serum 25-hydroxyvitamin D (25[OH]D) levels which indicate circulating Vitamin D levels. While serum 1.25(OH)D levels are less frequently performed, although serum 1.25(OH)D levels represent the active form of Vitamin D be a substitute for checking Vitamin D levels.

AIMS: This study aims to see the correlation between Vitamin D levels, namely, 25(OH)D and 1.25(OH)D saliva, which correlate with serum 25(OH)D and 1.25(OH)D levels so that the examination of salivary Vitamin D levels can be a substitute for checking serum Vitamin D levels.

MATERIAL AND METHODS: This study is a cross-sectional study involving healthy men and women, aged 20–50 years, sampling in Lima Puluh Village, Batubara District, North Sumatra Province, Indonesia. The parameters studied were 25(OH)D and 1.25(OH)D levels of saliva and serum.

RESULTS: This study involved 56 study subjects, male and female, with a percentage of deficiencies of 78.6% by examining 25(OH)D saliva and 76.8% by examining 25(OH)D serum. As for the 1.25(OH)D examination of saliva and serum, all were within normal limits. The analysis showed that a moderate correlation was obtained for levels of 25(OH)D saliva with serum 25(OH)D (p = 0.424) and a weak correlation for levels of 1.25(OH)D saliva with serum 25(OH)D (p = 0.339).

CONCLUSIONS: Salivary 25(OH)D assay can be used to replace serum 25(OH)D assay in healthy people as a non-invasive alternative.


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Sari DK, Mega JY, Harahap J. Nutrition status related to clinical improvement in AFB-positive pulmonary tuberculosis patients in primary health centres in Medan, Indonesia. Open Access Maced J Med Sci. 2019;7(10):1621-7. DOI:

Chaiprasongsuk A, Janjetovic Z, Kim TK, Jarrett SG, D’Orazio JA, Holick MF, et al. Protective effects of novel derivatives of Vitamin D3 and lumisterol against UVB-induced damage in human keratinocytes involve activation of Nrf2 and p53 defense mechanisms. Redox Biol. 2019;24:101206. PMid:31039479 DOI:

Alshahrani FM, Almalki MH, Aljohani N, Alzahrani A, Alsaleh Y, Holick MF. Vitamin D: Light side and best time of sunshine in Riyadh, Saudi Arabia. Dermatoendocrinol. 2013;5(1):177-80. PMid:24494051 DOI:

Ganmaa D, Munkhzul B, Fawzi W, Spiegelman D, Willett WC, Bayasgalan P, et al. High-dose Vitamin D3 during tuberculosis treatment in Mongolia. A randomized controlled trial. Am J Respir Crit Care Med. 2017;196(5):628-37. PMid:28692301 DOI:

McCullough PJ, Lehrer DS. Vitamin D, cod liver oil, sunshine, and phototherapy: Safe, effective and forgotten tools for treating and curing tuberculosis infections a comprehensive review. J Steroid Biochem Mol Biol. 2018;177:21-9. PMid:28756294 DOI:

Wahyunitisari MR, Mertaniasih NM, Amin M, Artama WT, Koendhori KB. Vitamin D, cell death pathways, and tuberculosis. Int J Mycobacteriol. 2017;6(4):349-55. PMid:29171448 DOI:

Bochen F, Balensiefer B, Körner S, Bittenbring JT, Neumann F, Koch A, et al. Vitamin D deficiency in head and neck cancer patients - prevalence, prognostic value and impact on immune function. Oncoimmunology. 2018;7(9):e1476817. PMid:30228945 DOI:

Fanidi A, Muller DC, Midttun O, Ueland PM, Vollset SE, Relton C, Vineis P, et al. Circulating Vitamin D in relation to cancer incidence and survival of the head and neck and oesophagus in the EPIC cohort. Sci Rep. 2016;6:36017. PMid:27812016 DOI:

Fairney A, Saphier PW. Studies on the measurement of 25-hydroxy Vitamin D in human saliva. Br J Nutr. 1987;57(1):13-25. PMid:3801379 DOI:

Gottlieb CW, Retief FP, Herbert V. Blockade of Vitamin B12-binding sites in gastric juice, serum and saliva by analogues and derivatives of Vitamin B12 and by antibody to intrinsic factor. Biochim Biophys Acta. 1967;141(3):560-72. https://doi. org/10.1016/0304-4165(67)90185-7 PMid:5233398 DOI:

Flusser J, Chvojkova V, Kozicka V. [Relationship between saliva and Vitamin B12. (Contribution to the problem of the intrinsic factor)]. Cas Lek Cesk. 1961;100:199-205. PMid:13700414

Heaney RP, Holick MF. Why the IOM recommendations for Vitamin D are deficient. J Bone Miner Res. 2011;26(3):455-7. PMid:21337617 DOI:

Grant WB, Holick MF. Benefits and requirements of Vitamin D for optimal health: A review. Altern Med Rev. 2005;10(2):94-111. PMid:15989379

Baggerly CA, Cuomo RE, French CB, Garland CF, Gorham ED, Grant WB, et al. Sunlight and Vitamin D: Necessary for public health. J Am Coll Nutr. 2015;34(4):359-65. PMid:26098394 DOI:

Cannell JJ, Grant WB, Holick MF. Vitamin D and inflammation. Dermatoendocrinol. 2014;6(1):e983401. PMid:26413186 DOI:

Cannell JJ, Vieth R, Umhau JC, Holick MF, Grant WB, Madronich S, et al. Epidemic influenza and Vitamin D. Epidemiol Infect. 2006;134(6):129-40. PMid:16959053 DOI:

Evans LW, Omaye ST. Use of saliva biomarkers to monitor efficacy of Vitamin C in exercise-induced oxidative stress. Antioxidants (Basel). 2017;6(1):5. PMid:28085082 DOI:

Rakshasbhuvankar AA, Clarke MW, Simmer K, Patole SK, Pillow JJ. Saliva for assessing Vitamin A status in extremely preterm infants: A diagnostic study. Neonatology. 2020;117(3):365-8. PMid:32114572 DOI:

Annweiler C, Kabeshova A, Callens A, Paty ML, Duval GT, Holick MF. Self-administered Vitamin D status predictor: Older adults are able to use a self-questionnaire for evaluating their Vitamin D status. PLoS One. 2017;12(11):e0186578. PMid:29091930 DOI:

Biancuzzo RM, Clarke N, Reitz RE, Travison TG, Holick MF. Serum concentrations of 1,25-dihydroxyvitamin D2 and 1,25-dihydroxyvitamin D3 in response to Vitamin D2 and Vitamin D3 supplementation. J Clin Endocrinol Metab. 2013;98(3):973-9. PMid:23386645 DOI:

Cocate PG, Kac G, Heitmann BL, Nadanovsky P, da Veiga Soares Carvalho MC, Benaim C, et al. Calcium and Vitamin D supplementation and/or periodontal therapy in the treatment of periodontitis among Brazilian pregnant women: Protocol of a feasibility randomised controlled trial (the IMPROVE trial). Pilot Feasibility Stud. 2019;5:38. PMid:30873290 DOI:

Figueiredo AC, Trujillo J, Freitas-Vilela AA, Franco-Sena AB, Rebelo F, Cunha GM, et al. Association between plasma concentrations of Vitamin D metabolites and depressive symptoms throughout pregnancy in a prospective cohort of Brazilian women. J Psychiatr Res. 2017;95:1-8. PMid:28755554 DOI:

Isola G, Polizzi A, Muraglie S, Leonardi R, Giudice AL. Assessment of Vitamin C and antioxidant profiles in saliva and serum in patients with periodontitis and ischemic heart disease. Nutrients. 2019;11(12):2956. PMid:31817129 DOI:

Balcells ME, García P, Tiznado C, Villarroel L, Scioscia N, Carvajal C, et al. Association of Vitamin D deficiency, season of the year, and latent tuberculosis infection among household contacts. PLoS One. 2017;12(4):e0175400. PMid:28403225 DOI:




How to Cite

Sari DK, Sari LM, Laksmi LI, Farhat F. The Use of 25-hydroxyvitamin D Saliva Test to Replace Vitamin D Serum Blood Test in Healthy People. Open Access Maced J Med Sci [Internet]. 2021 May 17 [cited 2024 Apr. 21];9(T3):40-3. Available from:

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