Biomarker of Oxidative Stress in Premature Hair Graying at Young Age




Premature hair graying, Young age, Oxidative stress


BACKGROUND: Gray hair is a physiological process of aging that occurs in everyone. Premature hair graying (PHG) is the term when early hair gray at an unusual age. The causes of PHG are multifactorial, genetic, nutritional, and environmental, including oxidative stress. Free radicals caused interference with cellular responses that cause direct damage to various proteins and DNA in the long term. The body’s defense mechanisms likely antioxidant enzymes, including catalase, glutathione peroxidase (GPx), and superoxide dismutase (SOD) was activated against free radicals.

AIM: We aimed to evaluate markers of oxidative stress and associated with severe graying at young age.

MATERIALS AND METHODS: We recruited consecutive 40 respondents with PHG and healthy controls, male sex and aged <25 years. The severe graying was graded with total of gray hair. The serum samples were collected to detect oxidative stress markers through malondialdehyde (MDA), SOD, catalase, and GPx measurement with enzyme-linked immunosorbent assay.

RESULTS: Serum MDA concentration was higher but not significantly (p > 0.05), while serum SOD, catalase, and GPx level, indicators of antioxidant were significantly lower (p < 0.001, p = 0.017 and p < 0.001, respectively) in PHG compared to controls. The correlation between oxidative stress and graying severity was not significant (p > 0.05), but the association between onset and severity of graying was significant (p < 0.001).

CONCLUSIONS: Respondents with PHG had increased of pro-oxidants and decreased of antioxidants compared than controls. The severity of graying is equivalent to the level of oxidative stress. The supplement of antioxidants is likely recommended in PHG.


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Trüeb RM. Pharmacological interventions in aging hair. Clin Interv Aging. 2006;1(2):121-9. PMid:18044109 DOI:

Anggraini DR, Feriyawati L, Hidayat H, Wahyuni AS. Risk factors associated with premature hair greying of young adult. Open Access Maced J Med Sci. 2019;7(22):3762-64. PMid:32127970 DOI:

Tobin DJ. Human hair pigmentation biological aspects. Int J Cosmet Sci. 2008;30(4):233-57. PMid:18713071 DOI:

Panhard S, Lozano I, Loussouarn G. Greying of human hair: A worldwide survey, revisiting the ‘50’ rule of thumb. Br J Dermatol. 2012;167(4):865-73. PMid:22716034 DOI:

Sehrawat M, Sinha S, Meena N, Sharma PK. Biology of hair pigmentation and its role in premature canities. Pigment Int. 2017;4(1):7-12. DOI:

Del Rio D, Stewart AJ, Pellegrini N. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis. 2005;15(4):316- 28. PMid:16054557 DOI:

Sitorus MS, Anggraini DR, Hidayat H. Decreasing free radicals level on high person after Vitamin C and E supplement treatment. IOP Conf Mater Sci Eng. 2017;180:1-8. DOI:

Noori S. An overview of oxidative stress and antioxidant defensive system. Open Access Sci Rep. 2012;1(8):1-9. DOI:

Young WK, Tatiana VB. Oxidative stress in angiogenesis and vacular disease. Blood. 2014;123(5):625-31. PMid:24300855 DOI:

Arck PC, Overall R, Spatz K, Liezman C, Handjiski B, Klapp BF, et al. Towards a “free radical theory of graying”: Melanocyte apoptosis in the aging human hair follicle is an indicator of oxidative stress induced tissue damage. FASEB J. 2018;20(9):1567-9. PMid:16723385 DOI:

Jo SJ, Paik SH, Choi JW, Lee JH, Cho S, Kim KH, et al. Hair graying pattern depends on gender, onset age and smoking habits. Acta Derm Venereol. 2012;92(2):160-1. PMid:22113716 DOI:

Daulatabad D, Singal A, Grover C, Chhillar N. Profile of Indian patients with premature canities Indian J Dermatol Venereol Leprol. 2016;82(2):169-72. PMid:26585843 DOI:

Daulatabad D, Singal A, Grover C, Sharma SB, Chhillar N. Assessment of oxidative stress in patients with premature canities. Int J Trichol. 2015;7(3):91-4. PMid:26622150 DOI:

Kauser S, Westgate GE, Green MR, Tobin DJ. Human hair follicle and epidermal melanocytes exhibit striking differences in their aging profile which involves catalase. J Invest Dermatol. 2011;131(4):979-82. PMid:21191398 DOI:

Saxena S, Gautam RK, Grupta A, Chitkara A. Evaluation of systemic oxidative stress in patients with premature canities and correlation of severity of hair graying with the degree of redox imbalance. Int J Trichol. 2020;12(1):16-23. PMid:32549695 DOI:

Singal A Daulatabad D, Grover C. Graying severity score: A useful tool for evaluation of a premature canities. Indian Dermatol Online J. 2016;7(3):164-7. PMid:27294049 DOI:

Shin H, Ryu HH, Yoon J, Jo S, Jang S, Choi M, et al. Association of premature hair graying with family history, smoking, and obesity: A cross-sectional study. J Am Acad Dermatol. 2015;72(2):321-7. PMid:25484268 DOI:

Margaritelis NV, Veskoukis AS, Paschalis V, Vrabas IS, Dipla K, Zafeiridis A, et al. Blood reflects tissue oxidative stress: A systematic review. Biomarkers. 2015;20(2):97-108. PMid:25582635 DOI:

Akin Belli A, Etgu F, Ozbas Gok S, Kara B, Dogan G. Risk factors for premature hair graying in young Turkish adults. Pediatr Dermatol. 2016;33(4):438-42. PMid:27292443 DOI:

Krugluger W, Stiefsohn K, Laciak K. Vit B 12 activates wnt pathway in human hair follicle by induction of b catenin and inhibition of glycogen synthase kinase 3 transcription. J Cosmet Dermatol Sci Appl. 2011;1:25-9. DOI:

Bhat RM, Sharma R, Pinto AC, Dandekeri S, Martis J. Epidemiological and investigative a study of premature graying of hair in higher secondary and pre-university school children. Int J Trichol. 2013;5(1):17-21. PMid:23960391 DOI:

Anggraini DR, Feriyawati L, Hidayat H. Serum ferritin and Vitamin D levels in premature hair graying of college student at the Universitas Sumatera Utara area. IOP Conf Earth Environ Sci. 2019;305:1-5. DOI:




How to Cite

Anggraini DR, Feriyawati L, Sitorus MS, Daulay M. Biomarker of Oxidative Stress in Premature Hair Graying at Young Age. Open Access Maced J Med Sci [Internet]. 2021 May 26 [cited 2024 Jun. 17];9(T3):44-7. Available from:

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