The Effect of Coffee Arabica Gayo Leaf Extract (Coffea arabica L.) on Increased PI3K Serum Levels in Type 2 Diabetic Rat

Sake Juli Martina; Aznan Lelo; Dharma Lindarto; Ratna Akbari Ganie; Muhammad Ichwan; Hanifah Yusuf; Syafruddin Ilyas; Iqbal Pahlevi Nasution

doi:10.3889/oamjms.2021.6344

Authors

Sake Juli Martina Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Universitas Sumatera Utara Hospital, Medan, Indonesia
Aznan Lelo Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Dharma Lindarto Department of Internal Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Ratna Akbari Ganie Department of Clinical Pathology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Muhammad Ichwan Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Hanifah Yusuf Department of Pharmacology, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia
Syafruddin Ilyas Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia
Iqbal Pahlevi Nasution Department of Pediatric Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.6344

Keywords:

PI3K, Coffee arabica gayo leaf extract, Type 2 diabetic

Abstract

BACKGROUND: Coffee arabica gayo leaf extract (Coffea arabica L.) contains polyphenols (chlorogenic acid), flavonoids, saponins, tannins, and steroids which improved PI3K serum levels in rats type 2 diabetic rat.

AIM: The purpose of the study was to determine the effect of Coffee Arabica Gayo Leaf Extract (C. arabica L.) on increased PI3K serum levels in type-2 diabetic rat (Rattus norvegicus).

METHODS: Thirty-five male Wistar rats with type 2 diabetic induced a combination of feeding a high-fat diet for 5 weeks and intraperitoneal injection of low dose streptozotocin (30 mg/kg). The diabetic rats were randomly divided into seven groups which consist of group without type 2 diabetic, group without type 2 diabetic with dose 250 mg/kg/day Coffee Arabica Gayo Leaf Extract, group with type 2 diabetic without intervention, group with type 2 diabetic with metformin, and group with type 2 diabetic with Coffee Arabica Gayo Leaf Extract doses 150, 200, and 250 mg/kg/day. The extract was administered orally for 30 days. Subsequently, PI3K serum levels were measure by Sandwich Elisa.

RESULTS: There were significantly higher PI3K serum levels in the treatment groups than in the control groups (p = 0.037) after giving Coffee Arabica Gayo Leaf Extract 200 mg/kg/day and 250 mg/kg/day to rats for 30 days.

CONCLUSION: These data suggest that Coffee Arabica Gayo Leaf Extract can improve PI3K serum levels in a rat model type 2 diabetic.

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References

International Diabetes Federation. IDF Diabetes Atlas. 7th ed. Belgium: International Diabetes Federation; 2019.

Perkumpulan Endokrinologi Indonesia. Konsensus Pengelolaan dan Pencegahan Diabetes Mellitus Tipe 2 di Indonesia, PB PERKENI; 2015.

Martina SJ, Govindan PA, Wahyuni AS. The difference in effect of Arabica coffee Gayo beans and leaf (Coffea arabica Gayo) extract on decreasing blood sugar levels in healthy mice. Open Access Maced J Med Sci. 2019;7(20):3363-5. https://doi.org/10.3889/oamjms.2019.423 DOI: https://doi.org/10.3889/oamjms.2019.423

Ristiana, D. Antioxidant activity and phenol content of various coffee leaf extracts (Coffea Sp.): Potential application of natural ingredients for food fortification. J Aplikasi Teknol Pangan. 2016;6(2):89-92. https://doi.org/10.17728/jatp.205 DOI: https://doi.org/10.17728/jatp.205

Herling AW, Schwab D, Burger HJ, Maas J, Hammerl R, Schmidt D, et al. Prolonged blood glucose reduction in mrp-2 deficient rats (GY/TR-) by the glucose-6-phosphate translocase inhibitor S 3025. Biochim Biophys Acta Gen Subj. 2002;1569(1-3):105-10. https://doi.org/10.1016/s0304-4165(01)00239-2 DOI: https://doi.org/10.1016/S0304-4165(01)00239-2

Marques V, Farah A. Chlorogenic acids and related compounds in medicinal plants and infusions. Food Chem. 2009;113(4):1370-6. https://doi.org/10.1016/j.foodchem.2008.08.086 DOI: https://doi.org/10.1016/j.foodchem.2008.08.086

Rodríguez-Gómez R, Vanheuverzwjin J, Souard F, Delporte C, Stevigny C, Stoffelen P, et al. Determination of three main chlorogenic acids in water extracts of coffee leaves by liquid chromatography coupled to an electrochemical detector. Antioxidants. 2018;7(10):143. https://doi.org/10.3390/antiox7100143 DOI: https://doi.org/10.3390/antiox7100143

Farhaty N, Muchtaridi M. Farmaka. J Unpad. 2016;14:214-27. Available from: http://www.jurnal.unpad.ac.id/farmaka. [Last accessed on 2020 Nov 12].

Federer WT. Experimental Design: Theory and Application. Pada: Hanafiah KA; 2010. Hanafiah KA. Rancangan Percobaan- Teori dan Aplikasi. 3rd ed. Jakarta: Raja Grafindo Persada. 1995. p. 9-10.

Vinayagam, R, Xu B. Antidiabetic properties of dietary flavonoids: A cellular mechanism review. BMC. 2015;12:60. DOI: https://doi.org/10.1186/s12986-015-0057-7

Jasmine R, Ganesh K, Rajaram R. Probing the mechanism of the antidiabetic potential of a terpenoid from Elephantopus scaber L., an Indian ethnomedicinal plant in STZ diabetic rats-in vivo and in silico analysis. Indian J Biochem Biophys. 2018;55:384-8.

Huang X, Liu G, Guo J, Su Z. The PI3K/AKT pathway in obesity and Type 2 diabetes. Int J Biol Sci. 2018;14(11):1483-96. https://doi.org/10.7150/ijbs.27173 PMid:30263000 DOI: https://doi.org/10.7150/ijbs.27173

Choi J, Kim KJ, Koh EJ, Lee BY. Gelidium elegans extract ameliorates Type 2 diabetes via regulation of MAPK and PI3K/ Akt signaling. Nutrients. 2018;10(1):51. https://doi.org/10.3390/nu10010051 PMid:29316644 DOI: https://doi.org/10.3390/nu10010051

Vergotine Z. Molecular Investigation of Genetic Factors Associated with Insulin Resistance and Obesity in a South African Population. South Africa: Stellenbosch University; 2015. Available from: https://www.scholar.sun.ac.za. [Last accessed on 2020 Nov 12].