Proliferative Activity of Myoepithelial Cells in Mucoepidermoid Carcinoma

Faisal Mehsen Alali; Bassel Tarakji; Nasser Raqe  Alqhtani; Abdullah Bin Nabhan; Ali Alrafedah; Adel  Alenzi; Nabil Kochaji

doi:10.3889/oamjms.2021.6368

Authors

Faisal Mehsen Alali Department of Oral and Maxillofacial Surgery, Diagnostic Sciences, Faculty College of Dentistry, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia; Department of Oral Histology and Pathology, Faculty of Dentistry, Damascus University, Syria https://orcid.org/0000-0001-6888-0639
Bassel Tarakji Department of Oral and Maxillofacial Surgery, Diagnostic Sciences, Faculty College of Dentistry, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia https://orcid.org/0000-0002-6029-0439
Nasser Raqe Alqhtani Department of Oral and Maxillofacial Surgery, Diagnostic Sciences, Faculty College of Dentistry, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia https://orcid.org/0000-0003-0877-6340
Abdullah Bin Nabhan Department of Oral and Maxillofacial Surgery, Diagnostic Sciences, Faculty College of Dentistry, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia
Ali Alrafedah Department of Oral and Maxillofacial Surgery, Diagnostic Sciences, Faculty College of Dentistry, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia https://orcid.org/0000-0002-4021-8552
Adel Alenzi Department of Oral and Maxillofacial Surgery, Diagnostic Sciences, Faculty College of Dentistry, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia
Nabil Kochaji Department of Oral Histology and Pathology, Faculty of Dentistry, Damascus University, Syria

DOI:

https://doi.org/10.3889/oamjms.2021.6368

Keywords:

Myoepithelial cells, Mucoepidermoid Carcinoma, PCNA, α-SMA, Double immunohistochemical labeling

Abstract

AIM: The aim of the study is to investigate the role of myoepithelial cells in the pathogenesis of mucoepidermoid carcinoma (MEC) using the double immunohistochemical staining; α _smooth muscle actin (_α-SMA)as specific marker for the myoepithelial cell differentiation and proliferative cell nuclear antigen (PCNA) as a marker for proliferative activity of myoepithelial cells.

MATERIAL AND METHODS: Retrospective study of twenty salivary gland specimens (ten MEC and ten normal salivary glands) were studied using double immunohistochemical labeling for α _smooth muscle actin α-SMA) and proliferative cell nuclear antigen (PCNA). The SPSS statistical package was used for data analysis (IBM SPSS Statistics for Windows, Version 20.0, Released 2011, IBM Corp, and Armonk, NY, USA).

RESULTS: In mucoepidermoid carcinomas, no positivity of α-SMA was seen in neoplastic cells (Frequent test), and it was just observed in the stroma of tumor, in the walls of blood vessels whereas, PCNA was positive, especially in high-grade tumors. In contrast, in normal salivary glands, the proliferating myoepithelial cells are stained by both α-SMA and PCNA.

CONCLUSIONS: We believe that the myoepithelial cells have no a role in the development of mucoepidermoid carcinoma.

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