Proliferative Activity of Myoepithelial Cells in Mucoepidermoid Carcinoma

Authors

  • Faisal Mehsen Alali Department of Oral and Maxillofacial Surgery, Diagnostic Sciences, Faculty College of Dentistry, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia; Department of Oral Histology and Pathology, Faculty of Dentistry, Damascus University, Syria https://orcid.org/0000-0001-6888-0639
  • Bassel Tarakji Department of Oral and Maxillofacial Surgery, Diagnostic Sciences, Faculty College of Dentistry, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia https://orcid.org/0000-0002-6029-0439
  • Nasser Raqe Alqhtani Department of Oral and Maxillofacial Surgery, Diagnostic Sciences, Faculty College of Dentistry, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia https://orcid.org/0000-0003-0877-6340
  • Abdullah Bin Nabhan Department of Oral and Maxillofacial Surgery, Diagnostic Sciences, Faculty College of Dentistry, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia
  • Ali Alrafedah Department of Oral and Maxillofacial Surgery, Diagnostic Sciences, Faculty College of Dentistry, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia https://orcid.org/0000-0002-4021-8552
  • Adel Alenzi Department of Oral and Maxillofacial Surgery, Diagnostic Sciences, Faculty College of Dentistry, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia
  • Nabil Kochaji Department of Oral Histology and Pathology, Faculty of Dentistry, Damascus University, Syria

DOI:

https://doi.org/10.3889/oamjms.2021.6368

Keywords:

Myoepithelial cells, Mucoepidermoid Carcinoma, PCNA, α-SMA, Double immunohistochemical labeling

Abstract

AIM: The aim of the study is to investigate the role of myoepithelial cells in the pathogenesis of mucoepidermoid carcinoma (MEC) using the double immunohistochemical staining; α _smooth muscle actin (_α-SMA)as specific marker for the myoepithelial cell differentiation and proliferative cell nuclear antigen (PCNA) as a marker for proliferative activity of myoepithelial cells.

MATERIAL AND METHODS: Retrospective study of twenty salivary gland specimens (ten MEC and ten normal salivary glands) were studied using double immunohistochemical labeling for α _smooth muscle actin α-SMA) and proliferative cell nuclear antigen (PCNA). The SPSS statistical package was used for data analysis (IBM SPSS Statistics for Windows, Version 20.0, Released 2011, IBM Corp, and Armonk, NY, USA).

RESULTS: In mucoepidermoid carcinomas, no positivity of α-SMA was seen in neoplastic cells (Frequent test), and it was just observed in the stroma of tumor, in the walls of blood vessels whereas, PCNA was positive, especially in high-grade tumors. In contrast, in normal salivary glands, the proliferating myoepithelial cells are stained by both α-SMA and PCNA.

CONCLUSIONS: We believe that the myoepithelial cells have no a role in the development of mucoepidermoid carcinoma.

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References

Gill S, Mohan A, Aggarwal S, Varshney A. Mucoepidermoid carcinoma of hard palate. Indian J Pathol Microbiol. 2018;61(3):397-8. https://doi.org/10.4103/ijpm.ijpm_617_17 PMid:30004063 DOI: https://doi.org/10.4103/IJPM.IJPM_617_17

Holmberg KV, Hoffman MP. Anatomy, biogenesis and regeneration of salivary glands. Monogr Oral Sci. 2014;24:1-13. PMid:24862590 DOI: https://doi.org/10.1159/000358776

Tata A, Kobayashi Y, Chow RD, Tran J, Desai A, Massri AJ, et al. Myoepithelial cells of submucosal glands can function as reserve stem cells to regenerate airways after injury. Cell Stem Cell. 2018;22(5):668-83.e6. https://doi.org/10.1016/j.stem.2018.03.018 PMid:29656943 DOI: https://doi.org/10.1016/j.stem.2018.03.018

Lynch TJ, Anderson PJ, Rotti PG, Tyler SR, Crooke AK, Choi SH, et al. Submucosal gland myoepithelial cells are reserve stem cells that can regenerate mouse tracheal epithelium. Cell Stem Cell. 2018;22(5):653-67.e5. https://doi.org/10.1016/j.stem.2018.03.017 PMid:29656941 DOI: https://doi.org/10.1016/j.stem.2018.03.017

Fonseca I, Félix A, Soares J. Cell proliferation in salivary gland adenocarcinomas with myoepithelial participation. A study of 78 cases. Virchows Arch. 1997;430(3):227-32. https://doi.org/10.1007/bf01324806 PMid:9099980 DOI: https://doi.org/10.1007/BF01324806

Balachander N, Masthan KM, Babu NA, Anbazhagan V. Myoepithelial cells in pathology. J Pharm Bioallied Sci. 2015;7 Suppl 1:S192-3. https://doi.org/10.4103/0975-7406.155898 PMid:26015706 DOI: https://doi.org/10.4103/0975-7406.155898

Savera AT, Zarbo RJ. Defining the role of myoepithelium in salivary gland neoplasia. Adv Anat Pathol. 2004;11(2):69-85. https://doi.org/10.1097/00125480-200403000-00001 PMid:15090843 DOI: https://doi.org/10.1097/00125480-200403000-00001

Chenevert J, Barnes LE, Chiosea SI. Mucoepidermoid carcinoma: A five-decade journey. Virchows Arch. 2011;458(2):133-40. https://doi.org/10.1007/s00428-011-1040-y PMid:21243374 DOI: https://doi.org/10.1007/s00428-011-1040-y

Yang L, Hashimura K, Qin C, Shrestha P, Sumitomo S, Mori M. Immunoreactivity of proliferating cell nuclear antigen in salivary gland tumours: An assessment of growth potential. Virchows Arch A Pathol Anat Histopathol. 1993;422(6):481-6. https://doi.org/10.1007/bf01606457 PMid:8101406 DOI: https://doi.org/10.1007/BF01606457

Cho K, Lee S, Lee Y. Proliferating cell nuclear antigen and C-ERBB-2 oncoprotein expression in adenoid cystic carcinomas of the salivary gland. Head Neck. 1999;21(5):414-9. https://doi.org/10.1002/(sici)1097-0347(199908)21:5<414:aid-hed6>3.0.co;2-m PMid:10402521 DOI: https://doi.org/10.1002/(SICI)1097-0347(199908)21:5<414::AID-HED6>3.0.CO;2-M

Prasad AR, Savera AT, Gown AM, Zarbo RJ. The myoepithelial immunophenotype in 135 Benign and pleomorphic adenoma. Arch Pathol Lab Med. 1999;123(9):801-6. https://doi.org/10.5858/1999-123-0801-tmiiba PMid:10458827 DOI: https://doi.org/10.5858/1999-123-0801-TMIIBA

Furuse C, de Sousa SO, Nunes FD, de Magalhaes MH, de Arauijo VC. Myoepithelial cell markers in salivary gland neoplasms. Int J Surg Pathol. 2005;13(1):57-65. https://doi.org/10.1177/106689690501300108 PMid:15735856 DOI: https://doi.org/10.1177/106689690501300108

Ihrler S, Zietz C, Sendelhofert A, Lang S, Blasenbreu-Vogt S, Löhrs U. A morphogenetic concept of salivary duct regeneration and metaplasia. Virchows Arch. 2002;440(5):519-26. https://doi.org/10.1007/s004280100537 PMid:12021927 DOI: https://doi.org/10.1007/s004280100537

Daniele E, Tralongo V, Morello V, Nagar C, Russo A, Bazan V, et al. Pleomorphic adenoma and adenoid-cystic carcinoma of salivary glands: Immunohistochemical assessment of proliferative activity in comparison with flow-cytometric study. Cell Prolif. 1996;29(3):153-62. https://doi.org/10.1046/j.1365-2184.1996.00991.x PMid:8652744 DOI: https://doi.org/10.1046/j.1365-2184.1996.00991.x

Ihrler S, Blasenbreu-Vogt S, Sendelhofert A, Rössle M, Harrison JD, Löhrs U. Regeneration in chronic sialadenitis: An analysis of proliferation and apoptosis based on double immunohistochemical labelling. Virchows Arch. 2004;444(4):356-61. https://doi.org/10.1007/s00428-003-0964-2 PMid:14762713 DOI: https://doi.org/10.1007/s00428-003-0964-2

Dardick I, Burford-Mason AP. Current status of histogenetic and morphogenetic concepts of salivary gland tumorigenesis. Crit Rev Oral Biol Med. 1993;4(5):639-77. https://doi.org/10.1177/10454411930040050201 PMid:8292713 DOI: https://doi.org/10.1177/10454411930040050201

Dardick I, Gliniecki MR, Heathcote JG, Burford-Mason A. Comparative histogenesis and morphogenesis of mucoepidermoid carcinoma and pleomorphic adenoma. An ultrastructural study. Virchows Arch A Pathol Anat Histopathol. 1990;417(5):405-17. https://doi.org/10.1007/bf01606029 PMid:2173253 DOI: https://doi.org/10.1007/BF01606029

Ogawa Y. Immunocytochemistry of myoepithelial cells in the salivary glands. Prog Histochem Cytochem. 2003;4:343-426. PMid:14509196 DOI: https://doi.org/10.1016/S0079-6336(03)80001-3

Patel KR, Solomon IH, El-Mofty SK, Lewis JS, Chernock RD. Mammaglobin and S-100 immunoreactivity in salivary gland carcinomas other than mammary analogue secretory carcinoma. Hum Pathol. 2013;44(11):2501-8. https://doi.org/10.1016/j.humpath.2013.06.010 PMid:24029710 DOI: https://doi.org/10.1016/j.humpath.2013.06.010

Ihrler S, Zietz C, Sendelhofert A, Riederer A, Löhrs U. Lymphoepithelial duct lesions in Sjögren-type sialadenitis. Virchows Arch. 1999;434(4):315-23. https://doi.org/10.1007/s004280050347 PMid:10335942 DOI: https://doi.org/10.1007/s004280050347

Dardick I. Mounting evidence against current histogenetic concepts for salivary gland tumorigenesis. Eur J Morphol. 1998;36:257-61. PMid:9825933

Savera AT, Sloman A, Huvos AG, Klimstra DS. Myoepithelial carcinoma of the salivary glands: A clinicopathologic study of 25 patients. Am J Surg Pathol. 2000;24(6):761-74. https://doi.org/10.1097/00000478-200006000-00001 PMid:10843278 DOI: https://doi.org/10.1097/00000478-200006000-00001

Capodiferro S, Ingravallo G, Limongelli L, Mastropasqua M, Tempesta A, Favia G, et al. Intra-cystic (in situ) mucoepidermoid carcinoma: A clinico-pathological study of 14 cases. J Clin Med. 2020;9(4):1157. https://doi.org/10.3390/jcm9041157 PMid:32325647 DOI: https://doi.org/10.3390/jcm9041157

Sawant PR, Spadigam A, Dhupar A, Syed S, Carvalho K. Assessing the prognostic significance of MUC4β _in mucoepidermoid carcinoma of the salivary glands: An immunohistochemical study. Heliyon. 2019;5(11):e02753. https://doi.org/10.1016/j.heliyon.2019.e02753 PMid:31844699 DOI: https://doi.org/10.1016/j.heliyon.2019.e02753

Triantafillidou K, Dimitrakopoulos J, Iordanidis F, Koufogiannis D. Mucoepidermoid carcinoma of minor salivary glands: A clinical study of 16 cases and review of the literature. Oral Dis. 2006;12(4):364-70. https://doi.org/10.1111/j.1601-0825.2005.01166.x PMid:16792720 DOI: https://doi.org/10.1111/j.1601-0825.2005.01166.x

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Published

2021-07-02

How to Cite

1.
Alali FM, Tarakji B, Alqhtani NR, Nabhan AB, Alrafedah A, Alenzi A, Kochaji N. Proliferative Activity of Myoepithelial Cells in Mucoepidermoid Carcinoma. Open Access Maced J Med Sci [Internet]. 2021 Jul. 2 [cited 2021 Sep. 20];9(A):451-4. Available from: https://oamjms.eu/index.php/mjms/article/view/6368

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