The Effect of Soy Milk on Mounting Latency, Mounting Frequency, and Reproductive Development in Male Wistar Rats (Rattus Norvegicus)

Nurdiana Nurdiana; Pradnyawati Chania; Rifzi Nurvitasari; Azmiatun Nisa; Styan Wahyu Diana; Evalina Izzatur Rochmah; Elly Mayangsari; Bambang Rahardjo; Wayan Indrawan; Husnul Khotimah; Karyono Mintaroem; Dicky Faizal Irnandi

doi:10.3889/oamjms.2021.6416

Authors

Nurdiana Nurdiana Laboratory of Pharmacology
Pradnyawati Chania Master Program of Midwifery, Faculty of Medicine, Brawijaya University, Malang, Indonesia; https://orcid.org/0000-0001-5745-6488
Rifzi Nurvitasari Master Program of Midwifery, Faculty of Medicine, Brawijaya University, Malang, Indonesia; https://orcid.org/0000-0001-9831-4347
Azmiatun Nisa Master Program of Midwifery, Faculty of Medicine, Brawijaya University, Malang, Indonesia; https://orcid.org/0000-0002-6333-3967
Styan Wahyu Diana Master Program of Midwifery, Faculty of Medicine, Brawijaya University, Malang, Indonesia; https://orcid.org/0000-0002-1914-9871
Evalina Izzatur Rochmah Bachelor Program of Medicine, Faculty of Medicine, Brawijaya University, Malang, Indonesia; https://orcid.org/0000-0001-8704-4898
Elly Mayangsari Laboratory of Pharmacology, Faculty of Medicine, Brawijaya University, Malang, Indonesia https://orcid.org/0000-0002-8604-5933
Bambang Rahardjo Department of Obstetrics and Gynecology
Wayan Indrawan Department of Obstetrics and Gynecology
Husnul Khotimah Laboratory of Pharmacology https://orcid.org/0000-0002-2374-4358
Karyono Mintaroem Laboratory of Anatomical Pathology https://orcid.org/0000-0002-7442-1401
Dicky Faizal Irnandi Laboratory of Biochemistry and Biomolecules, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia https://orcid.org/0000-0002-8636-8492

DOI:

https://doi.org/10.3889/oamjms.2021.6416

Keywords:

Soy milk, Endocrine-disrupting compounds, Mounting latency, Mounting frequency, Estrogen, Androgen-binding protein, Spermatozoa, Rattus norvegicus

Abstract

AIM: This research aims to examine the effects of soy milk on mounting latency (ML), mounting frequency (MF), estrogen levels, androgen-binding protein (ABP) expression, and spermatogenesis in male rats (Rattus norvegicus).

METHODS: Twenty-four male wistar rats (Rattus norvegicus) aged 4 weeks were divided into four groups. Control group (given a normal diet), P1; P2; P3 (given the normal diet and soy milk powder at doses of 7.1; 14.2; 21.3 g/KgBW/day, respectively) for 6 weeks. Observation of ML and MF were performed at 9 weeks 5 days of age, and rat surgery was performed at 10 weeks of age. Analysis of estrogen hormone levels was conducted by enzyme-linked immunosorbent assay (ELISA), ABP staining was using immunohistochemistry method, testicular spermatogenesis was observed using histopathological methods, and observation of spermatozoa was performed under the microscope.

RESULTS: The results showed no significant reduction of ML and MF, estrogen levels, and ABP expression (p ≤ 0.256; 0.865; 0.959, respectively) in male rat, but there was a significant decrease in the number, morphology, motility of spermatozoa, and testicular histophatology, (p ≤ 0.000, 0.003, 0.008, 0.000, respectively).

CONCLUSION: The administrassion of soy milk in various doses (7.1;14.2;21.3 g/KgBW/day) in male Wistar rats (Rattus norvegicus) had showed significantly difference on histopathological evaluation using Johnson’s scoring system, sperm quantity and quality, while on mounting latency and frequency, estrogen levels, and ABP expressions did not show significantly difference between groups. That describe of isoflavone in soy milk can affect several aspects related to male endocrine and reproductive development.

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Author Biographies

Nurdiana Nurdiana, Laboratory of Pharmacology

Pradnyawati Chania, Master Program of Midwifery, Faculty of Medicine, Brawijaya University, Malang, Indonesia;

Azmiatun Nisa, Master Program of Midwifery, Faculty of Medicine, Brawijaya University, Malang, Indonesia;

Evalina Izzatur Rochmah, Bachelor Program of Medicine, Faculty of Medicine, Brawijaya University, Malang, Indonesia;

Elly Mayangsari, Laboratory of Pharmacology, Faculty of Medicine, Brawijaya University, Malang, Indonesia

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