Bifidobacteria and Escherichia coli Microbiota of Healthy Indonesian Infants in Andalas Village: Profile of Infant Diet Given Exclusive Breastfed and Formula-fed

Imelda  Fitri; Eryati  Darwin; Eva Chundrayetti; Hotmauli Hotmauli; Eliya Mursyida; Titi Lasmini; Nurmi  Hasbi

doi:10.3889/oamjms.2021.6495

Authors

Imelda Fitri Department of Midwifery, Faculty of Pharmacy and Health Science, Universitas Abdurrab, Pekanbaru, Indonesia
Eryati Darwin Department of Histology, Faculty of Medicine, Universitas Andalas, Padang, Indonesia
Eva Chundrayetti Department of Pediatrics, Faculty of Medicine, Universitas Andalas, Padang, Indonesia
Hotmauli Hotmauli Department of Midwifery, Faculty of Pharmacy and Health Science, Universitas Abdurrab, Pekanbaru, Indonesia
Eliya Mursyida Department of Medicine, Faculty of Medicine, Universitas Abdurrab, Pekanbaru, Indonesia
Titi Lasmini Department of Medical Laboratory Technology, Health Academic John Paul, Pekanbaru, Indonesia
Nurmi Hasbi Department of Medical Laboratory Technology, Health Academic John Paul, Pekanbaru, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.6495

Keywords:

Bifidobacteria, Escherichia coli, Exclusive breastfed, Formula fed

Abstract

INTRODUCTION : Inbalance of microbiota in gastrointestinal tract have the risk of getting gastrointestinal infections, one of them is diarrhea.

AIM : The aim of this study was to determine bifidobacterium and eschericia coli microbiota and compare the microbiota obtained between exclusive breast fed and formula fed Indonesian infants village andalas.

METHODS : This study was an observational study with cross sectional comparative design. Samples faeses of infant was taken by Multistage Simple Random Sampling with total sample of 28 infants in range 0-6 months. This study was conducted in Pauh community health center areas, Village Andalas . The infant’s feces was taken then send to Microbiology Laboratorium of Dr. M. Djamil Hospital Padang city, Indonesia. The instruments used were a questionnaire and a colony counter. Data were processed and analyzed by using independent t test and Mann Whitney test.

RESULTS : The fecal microbiota of the 0-6 months Indonesian infants village andalas two species including bifidobacterium and eschericia coli were common commensal intestinal microbiota in all infants. The predominant intestinal microbiota in the breast fed infants bifidobacterium average 3,59 x 10⁹ CFU/g (p < 0,05). the mean eschericia coli microbiota from the formula fed infants 66,8 x 10⁹ CFU/g (p < 0,05)

CONCLUSION : Exclusive breastfeeding infant’s faeses contain more bifidobacteria and formula breastfed infant’s faeces contain more escherichia coli bacteria. These findings advance our understanding of the gut microbiota in healthy infants. They also provide new evidence infant diet as determinants of this essential microbial community in early life

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Kementrian Kesehatan Republik Indonesia. Profil Kesehatan Indonesia 2018. Jakarta: Kementerian Kesehatan Republik Indonesia; 2010. https://doi.org/10.6066/jtip.2013.24.2.121 DOI: https://doi.org/10.6066/jtip.2013.24.2.121

Brooks Geo F, Butel JS, Morse SA. Mikrobiologi Kedokteran. Jakarta: EGC; 2010.

Mitsuoka T. Intestinal flora and human health. Asia Pacific J Clin Nutr. 1996;5(1):2-9. PMid:24394457

Dinas Kesehatan Provinsi Sumatera Barat. Laporan Kinerja Dinas Kesehatan Prov Sumbar. Padang: Dinas Kesehatan Provinsi Sumatera Barat; 2017.

Pawlowski SW, Warren CA, Guerrant R. Diagnosis and treatment of acute or persistent diarrhea. Gastroenterology. 2009;136(6):1874-86. https://doi.org/10.1053/j.gastro.2009.02.072 PMid:19457416 DOI: https://doi.org/10.1053/j.gastro.2009.02.072

Morais TB, Morais MB, Sigulem DM. Bacterial contamination of the lacteal contents of feeding bottles in metropolitan Sao Paulo, Brazil. Bull World Health Organ. 1998;76(2):173-81. PMid:9648358

Wellstart International. Lactation Management Self-study Modules Level I. Shelburne, Vermont; 2013. Available from: https://www.wellstart.org/Self-Study-Module.pdf. [Last accessed on 2021 May 24 ].

Azard MB, Konya T, Maughan H, Guttman DS, Field CJ, Chari RS. Gut microbiota of healthy Canadian infant : Profiles by mode of delivery and infant diet at 4 months. CMAJ. 2013;185(5):385-94. https://doi.org/10.1503/cmaj.121189 PMid:23401405 DOI: https://doi.org/10.1503/cmaj.121189

Wasilewska E, Bielecka M. Isolation and identification of bifidobacteria from infant gut. Pol J Food Nutr Sci 2003;12(53):90-4.

Lievin V, Peiffer I, Hudault S, Rochat F, Brassart D, Neeser JR, et al. Bifidobacterium strains from resident infant human gastrointestinal microflora exert antimicrobial activity. Gut. 2000;47(5):646-52. https://doi.org/10.1136/gut.47.5.646 PMid:11034580 DOI: https://doi.org/10.1136/gut.47.5.646

Hadadji M, Benama R, Saidi N, Henni DE, Kihal M. Identification of cultivable Bifidobacterium species isolated from breast-fed infants feces in West-Algeria Miloud. African J Biotechnol. 2005;4(5):422-30. https://doi.org/10.17265/2159-5828/2012.10.005 DOI: https://doi.org/10.17265/2159-5828/2012.10.005

Hendraati PM, Kusharyati DF, Ryandini D, Oedjijono O. Characterization of bifidobacteria from infant feces with different mode of birth at Purwokerto, Indonesia. Biodiversitas. 2017;18(3):1265-9. https://doi.org/10.13057/biodiv/d180352 DOI: https://doi.org/10.13057/biodiv/d180352

Zinnah MA, Bari MR, Islam MT, Hossain MT., Rahman MT, Haque MH, et al. Characterization of Escherichia coli isolated from samples of different biological and environmental sources. Bangladesh J Vet Med. 2007;5(1-2):25-32. https://doi.org/10.3329/bjvm.v5i1.1305 DOI: https://doi.org/10.3329/bjvm.v5i1.1305

Fujimura KE, Slusher NA, Cabana MD, Lynch SV. Role of the gut microbiota in defining human health. Expert Rev Anti Infect Ther. 2010;8(4):435-54. https://doi.org/10.1586/eri.10.14 PMid:20377338 DOI: https://doi.org/10.1586/eri.10.14

Gaetano C. Immune development in late preterm neonates. Ital J Pediatr. 2014;40(2):A40. https://doi.org/10.1186/1824-7288-40-s2-a40 PMid:23734091 DOI: https://doi.org/10.1186/1824-7288-40-S2-A40

Wall R, Ross RP, Ryan CA, Hussey S, Murphy B, Fitzgerald GF, et al. Role of gut microbiota in early infant development. Clin Med Pediatr. 2009;3:45-54. https://doi.org/10.4137/cmped.s2008 PMid:23818794 DOI: https://doi.org/10.4137/CMPed.S2008

Irwanto EL, Yusrawati, Jurnalis YD, Roslaily R, Rizanda M. Effect of Spontaneous Delivery and Elective Caesarean Section on Number of Bifidobacterium Colony in Newborns. 2011;35(2):49–52. Available from: https://garuda.ristekbrin.go.id/documents/detail/768636

Erick P, Zimmermann K, Rusch K, Schwiertz A, Klosterhalfen S, Frick JS. The effects of maturation on the colonic microflora in infancy and childhood. Gastroenterol Res Pract. 2009;2009:752401. https://doi.org/10.1055/s-0028-1109055 PMid:19763278 DOI: https://doi.org/10.1055/s-0028-1109055

Prameela KK, Mohamed AE. Breast milk Immunoprotection and the common mucosal immune system: A review. Malays J Nutr. 2010;16(1):1-11. PMid:22691850

Rawal P, Gupta V, Thapa BR. Role of colostrum in gastrointestinal infections. Indian J Pediatr. 2008;75(9):917-21. PMid:19011803 DOI: https://doi.org/10.1007/s12098-008-0192-5

Penders J, Thijs C, Vink C, Stelma FF, Snijders B, Kummeling I, et al. Factors influencing the composition of the intestinal microbiota in early infancy. Pediatrics. 2006;118(2):511-21. https://doi.org/10.1542/peds.2005-2824 PMid:16882802 DOI: https://doi.org/10.1542/peds.2005-2824

Barcella L, Barbaro AP, Rogolino SB. Colonial morphology of Escherichia coli: Impact of detection in clinical specimens. Microbiol Med. 2016;31(2):51-5. https://doi.org/10.4081/mm.2016.5636 DOI: https://doi.org/10.4081/mm.2016.5636