Ramipril Increases Adma Concentration in Acute Myocardial Infarction in Rats Induced by Isoproterenol


  • Gestina Aliska Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Andalas, West Sumatra, Indonesia https://orcid.org/0000-0002-3234-898X
  • Alief Dhuha Department of Anatomy, Faculty of Medicine, Universitas Abdurrab, Pekanbaru, Riau, Indonesia https://orcid.org/0000-0002-4948-892X
  • Rahmatini Rahmatini Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Andalas, West Sumatra, Indonesia
  • Rita Hamdani Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Andalas, West Sumatra, Indonesia
  • Nita Afriani Department of Histology
  • Tofrizal Tofrizal Department of Anatomical Pathology, Faculty of Medicine, Universitas Andalas, West Sumatra, Indonesia
  • Hirowati Ali Department of Biochemistry, Faculty of Medicine, Universitas Andalas, West Sumatra, Indonesia
  • Nisa Pratiwi Medical Doctor Programme, Faculty of Medicine, Universitas Andalas, West Sumatra, Indonesia
  • Vinta Nuranisyah Medical Doctor Programme, Faculty of Medicine, Universitas Andalas, West Sumatra, Indonesia
  • Liganda Endo Mahata Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Andalas, West Sumatra, Indonesia https://orcid.org/0000-0002-3856-9003
  • Dedy Kurnia Department of Anesthesiology and Intensive Therapy, Faculty of Medicine, Universitas Andalas, West Sumatra, Indonesia




Asymmetric dimethylarginine, Dimethylarginine dimethylaminohydrolase, Nitric oxide, Myocardial infarction, Ramipril


In experimental animals, the induction of isoproterenol which is a synthetic of catecholamine, can cause acute myocardial infarction where the pathophysiology and morphology are the same as myocardial infarction in humans. Isoproterenol induction will increase oxidative stress, which will damage the enzyme dimethylarginine dimethylaminohydrolase (DDAH), thus causing asymmetric dimethylarginine (ADMA) levels to increase in circulation. Increased levels of ADMA will inhibit the activity of the enzyme nitric oxide synthase, which results in decreased nitric oxide resulting in endothelial damage. This study aims to determine the effect of Ramipril on asymmetric dimethylarginine (ADMA) levels in rats (Rattus norvegicus) Wistar strain with acute myocardial infarction.

Eighteen male Wistar rats (150-250 g) were randomly allocated into three groups: negative control group, positive control, and treatment group. The treatment group was pretreated with Ramipril at dose 3 mg/kg BW orally for seven days. Acute myocardial infarction was induced in positive control groups and treatment groups by subcutaneous injection of isoproterenol (85 mg/kg BW) for two consecutive days. Twenty-four hours after the last administration, rats from all groups were anesthetized and sacrificed for blood sample collection to evaluate the level of Asymmetric Dimethylarginine with Enzym-linked Immunosorbent Assay (ELISA) method. The result showed that ADMA levels were increased in the treatment group after pretreated with Ramipril. This study concluded that pretreatment with Ramipril increased ADMA concentration in acute myocardial infarction rats induced by isoproterenol.


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How to Cite

Aliska G, Dhuha A, Rahmatini R, Hamdani R, Afriani N, Tofrizal T, Ali H, Pratiwi N, Nuranisyah V, Mahata LE, Kurnia D. Ramipril Increases Adma Concentration in Acute Myocardial Infarction in Rats Induced by Isoproterenol. Open Access Maced J Med Sci [Internet]. 2021 Aug. 21 [cited 2023 May 31];9(A):693-7. Available from: https://oamjms.eu/index.php/mjms/article/view/6572

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